Anti-CLIPR-59抗体(ab74239)
Key features and details
- Rabbit polyclonal to CLIPR-59
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-CLIPR-59抗体
参阅全部 CLIPR-59 一抗 -
描述
兔多克隆抗体to CLIPR-59 -
宿主
Rabbit -
特异性
This antibody doesn't cross-react with human CLIP4, CLIP2 and CLIP1
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经测试应用
适用于: WB, ICC/IFmore details -
种属反应性
与反应: Human
预测可用于: Mouse
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免疫原
Synthetic peptide corresponding to Human CLIPR-59 (internal sequence).
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阳性对照
- A549 cells and extracts from A549 cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+ -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab74239于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (2) |
1/500 - 1/1000. Detects a band of approximately 60 kDa (predicted molecular weight: 60 kDa).
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| ICC/IF | (2) |
1/500 - 1/1000.
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| 说明 |
|---|
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WB
1/500 - 1/1000. Detects a band of approximately 60 kDa (predicted molecular weight: 60 kDa). |
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ICC/IF
1/500 - 1/1000. |
靶标
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功能
Functions as a cytoplasmic linker protein. Involved in TGN-endosome dynamics. -
序列相似性
Contains 3 ANK repeats.
Contains 2 CAP-Gly domains. -
结构域
Microtubule association is inhibited by the ANK repeats and the Golgi localization region (GoLD). -
细胞定位
Cytoplasm. Golgi apparatus > Golgi stack. Localized to Golgi stacks as well as on tubulovesicular elements juxtaposed to Golgi cisternae. - Information by UniProt
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数据库链接
- Entrez Gene: 25999 Human
- Entrez Gene: 76686 Mouse
- Omim: 607382 Human
- SwissProt: Q96DZ5 Human
- Unigene: 466539 Human
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别名
- 1500005P14Rik antibody
- AI844915 antibody
- CAP GLY domain containing linker protein 3 antibody
see all
图片
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Western blot - Anti-CLIPR-59 antibody (ab74239)All lanes : Anti-CLIPR-59 antibody (ab74239) at 1/500 dilution
Lane 1 : Extracts from A549 cells
Lane 2 : Extracts from A549 cells plus 5µg immunizing peptide
Lysates/proteins at 5 µg per lane.
Predicted band size: 60 kDa
Observed band size: 60 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-CLIPR-59 antibody (ab74239)Immunofluorescence analysis of A549 cells using ab74239 at a 1/500 dilution.
Left image un-treated.
Right image treated with immunizing peptide.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab74239 被引用在 2 文献中.
- Zepecki JP et al. miRNA-mediated loss of m6A increases nascent translation in glioblastoma. PLoS Genet 17:e1009086 (2021). PubMed: 33684100
- Zhou B et al. Long noncoding RNA LINC00284 facilitates cell proliferation in papillary thyroid cancer via impairing miR-3127-5p targeted E2F7 suppression. Cell Death Discov 7:156 (2021). PubMed: 34226533