Anti-CLIP170抗体[mAbcam61830] (ab61830)
![Western blot - Anti-CLIP170 antibody [mAbcam61830] (ab61830)](https://www.abcam.cn/ps/products/61/ab61830/Images/ab61830-482440-anti-clip170-antibody-western-blot-human.jpg "Western blot - Anti-CLIP170 antibody [mAbcam61830] (ab61830)")
Key features and details
- Mouse monoclonal [mAbcam61830] to CLIP170
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG1
概述
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产品名称
Anti-CLIP170抗体[mAbcam61830]
参阅全部 CLIP170 一抗 -
描述
小鼠单克隆抗体[mAbcam61830] to CLIP170 -
宿主
Mouse -
经测试应用
适用于: ICC/IF, WB, IHC-Pmore details
不适用于: Flow Cyt -
种属反应性
与反应: Human
预测可用于: Mouse, Rat
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免疫原
Recombinant fragment corresponding to Human CLIP170 aa 1100 to the C-terminus.
(Peptide available asab96632) -
阳性对照
- WB: Human skeletal muscle tissue lysate. ICC/IF: HEK293 cells. IHC-P: Human Cervical carcinoma tissue.
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常规说明
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine -
Concentration information loading... -
纯度
Protein G purified -
克隆
单克隆 -
克隆编号
mAbcam61830 -
骨髓瘤
Sp2/0-Ag14 -
同种型
IgG1 -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab61830于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF |
Use a concentration of 10 µg/ml.
|
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| WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 161 kDa (predicted molecular weight: 161 kDa).
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| IHC-P |
Use at an assay dependent concentration.
|
| 说明 |
|---|
|
ICC/IF
Use a concentration of 10 µg/ml. |
|
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 161 kDa (predicted molecular weight: 161 kDa). |
|
IHC-P
Use at an assay dependent concentration. |
靶标
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功能
Seems to be a intermediate filament associated protein that links endocytic vesicles to microtubules. -
组织特异性
Highly expressed in the Reed-Sternberg cells of Hodgkin disease. -
序列相似性
Contains 2 CAP-Gly domains. -
细胞定位
Cytoplasm. Cytoplasm > cytoskeleton. Associated with the cytoskeleton. Localizes to the tips of microtubules. - Information by UniProt
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数据库链接
- Entrez Gene: 6249 Human
- Entrez Gene: 56430 Mouse
- Entrez Gene: 65201 Rat
- Omim: 179838 Human
- SwissProt: P30622 Human
- SwissProt: Q922J3 Mouse
- Unigene: 524809 Human
- Unigene: 241109 Mouse
see all -
别名
- CAP GLY domain containing linker protein 1 antibody
- CAP-Gly domain-containing linker protein 1 antibody
- CLIP 170 antibody
see all
图片
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Western blot - Anti-CLIP170 antibody [mAbcam61830] (ab61830)Anti-CLIP170 antibody [mAbcam61830] (ab61830) at 1 µg/ml + Human Skeletal Muscle at 10 µg
Secondary
Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/5000 dilution
Predicted band size: 161 kDa
Observed band size: 150,161,190 kDa why is the actual band size different from the predicted?
Exposure time: 4 minutesBlocking buffer: 3% Milk
Gel type: TA
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![Immunocytochemistry/ Immunofluorescence - Anti-CLIP170 antibody [mAbcam61830] (ab61830)](https://www.abcam.cn/ps/products/61/ab61830/Images/ab61830-75145-anti-clip170-antibody-mabcam61830-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-CLIP170 antibody [mAbcam61830] (ab61830)ICC/IF image of ab61830 stained Hek293 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab61830 at 10µg/ml overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti- mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in HeLa, HepG2, and MCF-7 cell lines in PFA at 10ug/ml
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLIP170 antibody [mAbcam61830] (ab61830)](https://www.abcam.cn/ps/products/61/ab61830/Images/ab61830-75144-anti-clip170-antibody-mabcam61830-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLIP170 antibody [mAbcam61830] (ab61830)IHC image of Ab61830 staining in Human Cervical carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with Ab61830, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
实验方案
数据表及文件
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Datasheet download
文献 (1)
ab61830 被引用在 1 文献中.
- Li H et al. Human Placental Endothelial Cell and Trophoblast Heterogeneity and Differentiation Revealed by Single-Cell RNA Sequencing. Cells 12:N/A (2022). PubMed: 36611882
