重组Anti-CLIC3抗体[EPR8243(B)] (ab128941)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8243(B)] to CLIC3
- Suitable for: WB, IHC-P
- Reacts with: Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-CLIC3抗体[EPR8243(B)]
参阅全部 CLIC3 一抗 -
描述
兔单克隆抗体[EPR8243(B)] to CLIC3 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details
不适用于: Flow Cyt,ICC/IF or IP -
种属反应性
与反应: Rat, Human
预测可用于: Mouse -
免疫原
Synthetic peptide within Human CLIC3 aa 50-150. The exact sequence is proprietary.
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阳性对照
- JAR cell lysate; Rat lung tissue lysate; Human placenta tissue
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR8243(B) -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab128941于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/1000 - 1/10000. Detects a band of approximately 27 kDa (predicted molecular weight: 27 kDa).
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IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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说明 |
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WB
1/1000 - 1/10000. Detects a band of approximately 27 kDa (predicted molecular weight: 27 kDa). |
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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相关性
Chloride channels are a diverse group of proteins that regulate fundamental cellular processes including stabilization of cell membrane potential, transepithelial transport, maintenance of intracellular pH, and regulation of cell volume. Chloride intracellular channel 3 is a member of the p64 family and is predominantly localized in the nucleus and stimulates chloride ion channel activity. In addition, this protein may participate in cellular growth control, based on its association with ERK7, a member of the MAP kinase family. -
细胞定位
Nuclear, cytoplasmic and Single pass membrane. Predominantly nuclear but also found in the cytoplasm. Exists both as soluble cytoplasmic protein and as membrane protein with probably a single transmembrane domain -
数据库链接
- Entrez Gene: 9022 Human
- Entrez Gene: 69454 Mouse
- Entrez Gene: 296566 Rat
- Omim: 606533 Human
- SwissProt: O95833 Human
- SwissProt: Q9D7P7 Mouse
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别名
- 2300003G24Rik antibody
- Chloride intracellular channel 3 antibody
- Chloride intracellular channel protein 3 antibody
see all
图片
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All lanes : Anti-CLIC3 antibody [EPR8243(B)] (ab128941) at 1/1000 dilution
Lane 1 : JAR cell lysate
Lane 2 : Rat lung tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 27 kDa
Observed band size: 27 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLIC3 antibody [EPR8243(B)] (ab128941)
ab128941 at 1/50 staining CLIC3 in paraffin-embedded Human placenta tissue by immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab128941 被引用在 1 文献中.
- Kawai S et al. Pathophysiological properties of CLIC3 chloride channel in human gastric cancer cells. J Physiol Sci 70:15 (2020). PubMed: 32066374