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重组RabMAb

重组Anti-CLIC2抗体[EPR6494] (ab175230)

Submit a review Submit a question References (3)
Western blot - Anti-CLIC2 antibody [EPR6494] (ab175230)
  • Immunoprecipitation - Anti-CLIC2 antibody [EPR6494] (ab175230)
  • Immunocytochemistry/ Immunofluorescence - Anti-CLIC2 antibody [EPR6494] (ab175230)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLIC2 antibody [EPR6494] (ab175230)
  • Anti-CLIC2 antibody [EPR6494] (ab175230)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6494] to CLIC2
  • Suitable for: ICC/IF, IP, IHC-P, WB
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

概述

  • 产品名称

    Anti-CLIC2抗体[EPR6494]
    参阅全部 CLIC2 一抗

  • 描述

    兔单克隆抗体[EPR6494] to CLIC2

  • 宿主

    Rabbit

  • 特异性

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.

  • 经测试应用

    适用于: ICC/IF, IP, IHC-P, WBmore details

  • 种属反应性

    与反应: Human

  • 免疫原

    Synthetic peptide within Human CLIC2 aa 1-100 (Cysteine residue). The exact sequence is proprietary.
    Database link: O15247

  • 阳性对照

    • Human fetal liver, Human heart and Human testis lysates; Human liver tissue; HepG2 cells.

  • 常规说明

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • 存储溶液

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • 纯度

    Protein A purified

  • 克隆

    单克隆

  • 克隆编号

    EPR6494

  • 同种型

    IgG

  • 研究领域

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab175230于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
ICC/IF
1/100 - 1/250.
IP
1/10 - 1/100.
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB
1/1000 - 1/10000. Predicted molecular weight: 28 kDa.
说明
ICC/IF
1/100 - 1/250.
IP
1/10 - 1/100.
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB
1/1000 - 1/10000. Predicted molecular weight: 28 kDa.

靶标

  • 功能

    Can insert into membranes and form chloride ion channels. Channel activity depends on the pH. Membrane insertion seems to be redox-regulated and may occur only under oxydizing conditions. Modulates the activity of RYR2 and inhibits calcium influx.

  • 组织特异性

    Detected in adult brain, heart, liver, lung, spleen, stomach and testis. Expressed in fetal liver and adult skeletal muscle.

  • 序列相似性

    Belongs to the chloride channel CLIC family.
    Contains 1 GST C-terminal domain.

  • 结构域

    Members of this family may change from a globular, soluble state to a state where the N-terminal domain is inserted into the membrane and functions as chloride channel. A conformation change of the N-terminal domain is thought to expose hydrophobic surfaces that trigger membrane insertion.

  • 细胞定位

    Cytoplasm. Membrane. Exists both as soluble cytoplasmic protein and as membrane protein with probably a single transmembrane domain.
  • Information by UniProt

  • 数据库链接

    • 别名

      • Chloride intracellular channel 2 antibody
      • Chloride intracellular channel protein 2 antibody
      • CLIC 2b antibody
      • CLIC2 antibody
      • CLIC2_HUMAN antibody
      • CLIC2b antibody
      • MRXS32 antibody
      • OTTHUMP00000196471 antibody
      • XAP 121 antibody
      • XAP121 antibody
      see all

    图片

    • Western blot - Anti-CLIC2 antibody [EPR6494] (ab175230)
      Western blot - Anti-CLIC2 antibody [EPR6494] (ab175230)
      All lanes : Anti-CLIC2 antibody [EPR6494] (ab175230) at 1/1000 dilution

      Lane 1 : Human fetal liver tissue lysate
      Lane 2 : Human heart tissue lysate
      Lane 3 : Human testis tissue lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 28 kDa

    • Immunoprecipitation - Anti-CLIC2 antibody [EPR6494] (ab175230)
      Immunoprecipitation - Anti-CLIC2 antibody [EPR6494] (ab175230)

      Western blot analysis on immunoprecipitation pellet from (1) fetal liver lysate or (2) 1X PBS (negative control) using ab175230, and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

    • Immunocytochemistry/ Immunofluorescence - Anti-CLIC2 antibody [EPR6494] (ab175230)
      Immunocytochemistry/ Immunofluorescence - Anti-CLIC2 antibody [EPR6494] (ab175230)

      Immunofluorescent analysis of HepG2 cells labeling CLIC2 with ab175230 at 1/100 dilution (red). DAPI nuclear staining (blue).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLIC2 antibody [EPR6494] (ab175230)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLIC2 antibody [EPR6494] (ab175230)

      Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling CLIC2 with ab175230 at 1/50 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Anti-CLIC2 antibody [EPR6494] (ab175230)
      Anti-CLIC2 antibody [EPR6494] (ab175230)

    文献 (3)

    发表研究结果有使用 ab175230？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab175230 被引用在 3 文献中.

    • Dai X  et al. SLC26A4 Mutation Promotes Cell Apoptosis by Inducing Pendrin Transfer, Reducing Cl- Transport, and Inhibiting PI3K/Akt/mTOR Pathway. Biomed Res Int 2022:6496799 (2022). PubMed: 36072472
    • Liu G  et al. Analysis of Differentially Expressed Genes in a Chinese Cohort of Esophageal Squamous Cell Carcinoma. J Cancer 11:3783-3793 (2020). PubMed: 32328183
    • Xu T  et al. Chloride intracellular channel protein 2: prognostic marker and correlation with PD-1/PD-L1 in breast cancer. Aging (Albany NY) 12:17305-17327 (2020). PubMed: 32915772

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