AB307661

重组Anti-CLEC12A抗体[EPR26967-14] - BSA and Azide free

Anti-CLEC12A antibody [EPR26967-14] - BSA and Azide free

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal CLEC12A antibody. Carrier free. Suitable for WB and reacts with Human samples.

查看别名

CD371, CLL1, DCAL2, KLRL1, MICL, CLEC12A, C-type lectin domain family 12 member A, C-type lectin-like molecule 1, Dendritic cell-associated lectin 2, Killer cell C-type lectin-like receptor L1, Myeloid inhibitory C-type lectin-like receptor, CLL-1, DCAL-2, hKLRL1

2 Images

Supplier Data

Western blot - Anti-CLEC12A antibody [EPR26967-14] - BSA and Azide free (AB307661)

This data was developed using ab307660, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST Negative control : K562, Jurkat. This antibody only recognises glycosylated CLEC12A. Deglycosylated CLEC12A is not detected by WB with ab307660. In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution. Exposure time : 70 seconds

All lanes:

Western blot - Anti-CLEC12A antibody [EPR26967-14] (<a href='/products/primary-antibodies/clec12a-antibody-epr26967-14-ab307660'>ab307660</a>) at 1/1000 dilution

Lane 1:

THP-1 (human monocytic leukemia monocyte) whole cell lysate at 20 µg

Lane 2:

K562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate at 20 µg

Lane 3:

Jurkat (human T cell leukemia T lymphocyte) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 40 kDa,80 kDa

false

Exposure time: 70s

Supplier Data

Western blot - Anti-CLEC12A antibody [EPR26967-14] - BSA and Azide free (AB307661)

This data was developed using ab307660, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

CLEC12A is a glycosylated protein (glycoprotein) and can be deglycosylated by Protein Deglycosylation MIX II.

This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.

In Western blot, anti- Vinculin antibody (ab129002) loading control staining at 1/10000 dilution.

All lanes:

Western blot - Anti-CLEC12A antibody [EPR26967-14] (<a href='/products/primary-antibodies/clec12a-antibody-epr26967-14-ab307660'>ab307660</a>) at 1/1000 dilution

Lane 1:

Untreated U937 (human histiocytic lymphoma monocyte) whole cell lysate at 20 µg

Lane 2:

U937 whole cell lysate treated with Protein Deglycosylation Mix II at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 40-80 kDa

true

Exposure time: 147s

不同偶联物与剂型 (1)

Unconjugated

Anti-CLEC12A antibody [EPR26967-14]

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR26967-14

亚型

IgG

不含载体蛋白

Yes

反应种属

Human

应用

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

特异性

This antibody only recognises glycosylated CLEC12A. Deglycosylated CLEC12A is not detected by WB with ab307660.

The antibody only detects the glycosylated version.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Mouse": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Rat": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" } } }

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产品详情

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Constituents: PBS

运输条件

Blue Ice

产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Myeloid inhibitory C-type lectin receptor that acts as a negative regulator of myeloid cell activation (PubMed : 14739280, PubMed : 15238421, PubMed : 16239426, PubMed : 34234773, PubMed : 38367667, PubMed : 38386511, PubMed : 39143217). Myeloid cell inhibition is required to limit proinflammatory pathways and protect against excessive inflammation (By similarity). Specifically recognizes and binds various structures, such as neutrophil extracellular traps (NETs) or monosodium urate crystals (PubMed : 38367667, PubMed : 38386511, PubMed : 39143217). Also acts as a pattern-recognition receptor for pathogen-associated molecules, such as plasmodium hemozoin or mycobacterial micolic acid (PubMed : 31269448, PubMed : 36542980). Ligand-binding induces phosphorylation of its ITIM motif, followed by recruitment of tyrosine-protein phosphatases PTPN6 and PTPN11, which counteract tyrosine-protein kinase SYK, thereby preventing myeloid cell activation (PubMed : 14739280, PubMed : 16239426, PubMed : 34234773). Acts as a pattern-recognition receptor for NETs in neutrophils : specifically recognizes DNA in NETs, leading to inhibit neutrophil activation and limit further NET formation (PubMed : 39143217). This regulation is essential for controlling key neutrophil responses and limit NET-mediated inflammatory conditions (By similarity). Also recognizes dead cells by acting as a receptor for monosodium urate crystals, leading to down-regulate neutrophil activation (PubMed : 38367667, PubMed : 38386511). Binding to monosodium urate crystals also promotes the type I interferon response (By similarity). Acts as an inhibitor of natural killer (NK) cell cytotoxicity (PubMed : 15238421). Also acts as an inhibitor of dendritic cell maturation in an IL10-dependent manner (PubMed : 16239426).

See full target information CLEC12A

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Abcam Product Promise

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重组Anti-CLEC12A抗体[EPR26967-14] - BSA and Azide free