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AB248435

重组Anti-Cleaved Lamin A + C (Asp230)抗体[EPR4520-11] - BSA and Azide free

Anti-Cleaved Lamin A + C (Asp230) antibody [EPR4520-11] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal Lamin-A/C antibody. Carrier free. Suitable for WB and reacts with Mouse, Human samples. Cited in 1 publication.

查看别名

LMN1, LMNA, Prelamin-A/C

1 Images
Western blot - Anti-Cleaved Lamin A + C (Asp230) antibody [EPR4520-11] - BSA and Azide free (AB248435)
  • WB

Unknown

Western blot - Anti-Cleaved Lamin A + C (Asp230) antibody [EPR4520-11] - BSA and Azide free (AB248435)

This data was developed using ab133269, the same antibody clone in a different buffer formulation.

Note : The observed bands are possible cleavage fragments.

All lanes:

Western blot - Anti-Cleaved Lamin A + C (Asp230) antibody [EPR4520-11] (<a href='/products/primary-antibodies/cleaved-lamin-a-c-asp230-antibody-epr4520-11-ab133269'>ab133269</a>) at 1/50000 dilution

Lane 1:

Lysate of NIH 3T3 cells treated with staurosporine at 10 µg

Lane 2:

NIH 3T3 cell lysate at 10 µg

Lane 3:

Lysate of HeLa cells treated with camptothecine at 10 µg

Lane 4:

HeLa cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution

Predicted band size: 74 kDa

Observed band size: 45-50 kDa

false

不同偶联物与剂型 (1)

  • Unconjugated

    Anti-Cleaved Lamin A + C (Asp230) antibody [EPR4520-11]

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR4520-11

亚型

IgG

不含载体蛋白

Yes

反应种属

Mouse, Human

应用

WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Rat": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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产品详情

ab248435 is the carrier-free version of ab133269.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Protein A
存储溶液
pH: 7.2 - 7.4 Constituents: PBS
运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
+4°C
储存信息
Do Not Freeze
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Cleaved Lamin A + C (Asp230) also known as Progerin refers to a post-translationally modified form of A-type lamins that results from caspase-mediated cleavage at Asp230. The mass of Lamin A is approximately 74 kDa while Lamin C is around 65 kDa. These proteins mainly localize to the nuclear lamina a structural layer beneath the inner nuclear membrane. They express in a variety of tissues but are particularly abundant in cells with a high demand for nuclear structural integrity such as skin heart and muscle tissues.
Biological function summary

These cleaved lamins play a pivotal role in maintaining nuclear shape and stability. They serve as integral components of the nuclear lamina which is part of a complex scaffold that provides mechanical support to the nucleus. Cleaved Lamin A + C also aids in chromatin organization influencing gene expression. Their function impacts key cellular processes like DNA replication and repair as well as regulation of the cell cycle.

Pathways

The involvement of Cleaved Lamin A + C is important within apoptosis and cellular senescence pathways. They interact with proteins such as Caspase-6 and Caspase-7 in these processes. Lamin cleavage leads to nuclear disassembly during programmed cell death while in cellular senescence it signals alterations in nuclear integrity that influence aging-related pathways.

Cleaved Lamin A + C associates with accelerated aging disorders such as Hutchinson-Gilford Progeria Syndrome (HGPS). In this condition the accumulation of mutated lamin proteins results in defective nuclear architecture. Disorders like muscular dystrophy present a link as well where abnormal proteins like Emerin and Nesprin could interact with lamins affecting muscle cell viability and function.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Lamin-A/C. Lamins are intermediate filament proteins that assemble into a filamentous meshwork, and which constitute the major components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane (PubMed : 10080180, PubMed : 10580070, PubMed : 10587585, PubMed : 10814726, PubMed : 11799477, PubMed : 12075506, PubMed : 12927431, PubMed : 15317753, PubMed : 18551513, PubMed : 18611980, PubMed : 2188730, PubMed : 22431096, PubMed : 2344612, PubMed : 23666920, PubMed : 24741066, PubMed : 31434876, PubMed : 31548606, PubMed : 37788673, PubMed : 37832547). Lamins provide a framework for the nuclear envelope, bridging the nuclear envelope and chromatin, thereby playing an important role in nuclear assembly, chromatin organization, nuclear membrane and telomere dynamics (PubMed : 10080180, PubMed : 10580070, PubMed : 10587585, PubMed : 10814726, PubMed : 11799477, PubMed : 12075506, PubMed : 12927431, PubMed : 15317753, PubMed : 18551513, PubMed : 18611980, PubMed : 22431096, PubMed : 23666920, PubMed : 24741066, PubMed : 31548606, PubMed : 37788673, PubMed : 37832547). Lamin A and C also regulate matrix stiffness by conferring nuclear mechanical properties (PubMed : 23990565, PubMed : 25127216). The structural integrity of the lamina is strictly controlled by the cell cycle, as seen by the disintegration and formation of the nuclear envelope in prophase and telophase, respectively (PubMed : 2188730, PubMed : 2344612). Lamin A and C are present in equal amounts in the lamina of mammals (PubMed : 10080180, PubMed : 10580070, PubMed : 10587585, PubMed : 10814726, PubMed : 11799477, PubMed : 12075506, PubMed : 12927431, PubMed : 15317753, PubMed : 18551513, PubMed : 18611980, PubMed : 22431096, PubMed : 23666920, PubMed : 31548606). Also invoved in DNA repair : recruited by DNA repair proteins XRCC4 and IFFO1 to the DNA double-strand breaks (DSBs) to prevent chromosome translocation by immobilizing broken DNA ends (PubMed : 31548606). Required for normal development of peripheral nervous system and skeletal muscle and for muscle satellite cell proliferation (PubMed : 10080180, PubMed : 10814726, PubMed : 11799477, PubMed : 18551513, PubMed : 22431096). Required for osteoblastogenesis and bone formation (PubMed : 12075506, PubMed : 15317753, PubMed : 18611980). Also prevents fat infiltration of muscle and bone marrow, helping to maintain the volume and strength of skeletal muscle and bone (PubMed : 10587585). Required for cardiac homeostasis (PubMed : 10580070, PubMed : 12927431, PubMed : 18611980, PubMed : 23666920).. Prelamin-A/C. Prelamin-A/C can accelerate smooth muscle cell senescence (PubMed : 20458013). It acts to disrupt mitosis and induce DNA damage in vascular smooth muscle cells (VSMCs), leading to mitotic failure, genomic instability, and premature senescence (PubMed : 20458013).
See full target information LMNA
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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Oncology letters 24:457 PubMed36380881

2022

Suppressive effect of YY1-mediated RGS22 regulation on the proliferation, migration and invasion of pancreatic ductal adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Shou-Ji Cao,Wan-Li Ge,Ling-Dong Meng,Qun Chen,Yi Miao,Kui-Rong Jiang,Jing-Jing Zhang
View all publications
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搭配使用产品

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