重组Anti-Cleaved Lamin A + C (Asp230)抗体[EPR4520-11] (ab133269)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4520-11] to Cleaved Lamin A + C (Asp230)
- Suitable for: WB
- Reacts with: Mouse, Human
Related conjugates and formulations
概述
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产品名称
Anti-Cleaved Lamin A + C (Asp230)抗体[EPR4520-11]
参阅全部 Cleaved Lamin A + C (Asp230) 一抗 -
描述
兔单克隆抗体[EPR4520-11] to Cleaved Lamin A + C (Asp230) -
宿主
Rabbit -
经测试应用
适用于: WBmore details
不适用于: Flow Cyt,ICC/IF or IHC-P -
种属反应性
与反应: Mouse, Human
预测可用于: Rat -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- Lysate of NIH/3T3 cells treated with staurosporine, NIH/3T3 cell lysate, lysate of HeLa cells treated with camptothecine, HeLa whole cell lysate (ab150035)
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR4520-11 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab133269于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/10000 - 1/50000. Detects a band of approximately 45-50 kDa (predicted molecular weight: 74 kDa).
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说明 |
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WB
1/10000 - 1/50000. Detects a band of approximately 45-50 kDa (predicted molecular weight: 74 kDa). |
图片
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All lanes : Anti-Cleaved Lamin A + C (Asp230) antibody [EPR4520-11] (ab133269) at 1/50000 dilution
Lane 1 : Lysate of NIH 3T3 cells treated with staurosporine
Lane 2 : NIH 3T3 cell lysate
Lane 3 : Lysate of HeLa cells treated with camptothecine
Lane 4 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution
Predicted band size: 74 kDa
Observed band size: 45-50 kDa why is the actual band size different from the predicted?
Note: The observed bands are possible cleavage fragments.
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab133269 被引用在 2 文献中.
- Elmallah MIY et al. Marine Actinomycetes-Derived Secondary Metabolites Overcome TRAIL-Resistance via the Intrinsic Pathway through Downregulation of Survivin and XIAP. Cells 9:N/A (2020). PubMed: 32708048
- Dufour F et al. TRAIL receptor gene editing unveils TRAIL-R1 as a master player of apoptosis induced by TRAIL and ER stress. Oncotarget 8:9974-9985 (2017). PubMed: 28039489