重组Anti-Cleaved Caspase-7抗体[EPR22840-25] (ab256469)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22840-25] to Cleaved Caspase-7
- Suitable for: WB, IP
- Knockout validated
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-Cleaved Caspase-7抗体[EPR22840-25]
参阅全部 Cleaved Caspase-7 一抗 -
描述
兔单克隆抗体[EPR22840-25] to Cleaved Caspase-7 -
宿主
Rabbit -
经测试应用
适用于: WB, IPmore details
不适用于: Flow Cyt,ICC/IF or IHC-P -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: HeLa treated with 1 µM staurosporine for 3 hours whole cell lysate, Wild-type HAP1 treated with 1 µM staurosporine for 3 hours whole cell lysate, mouse and rat spleen whole cell lysate. IP: HeLa treated with 1 µM Staurosporine for 3 hours whole cell lysate .
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR22840-25 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab256469于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/1000. Detects a band of approximately 22, 18 kDa (predicted molecular weight: 34 kDa).
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IP |
1/30.
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说明 |
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WB
1/1000. Detects a band of approximately 22, 18 kDa (predicted molecular weight: 34 kDa). |
IP
1/30. |
靶标
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相关性
Caspases are cysteine proteases, expressed as inactive precursors, that mediate apoptosis by proteolysis of specific substrates. Caspases have the ability to cleave after aspartic acid residues. There are two classes of caspases involved in apoptosis; initiators (activation by receptor cluster) and effectors (activation by mitochondrial permeability transition). Proapoptotic signals autocatalytically activate initiator caspases, such as Caspase 8 and Caspase 9. Activated initiator caspases then process effector caspases, such as Caspase 3 and Caspase 7, which in turn cause cell collapse. -
细胞定位
Cytoplasmic -
数据库链接
- Entrez Gene: 840 Human
- Omim: 601761 Human
- SwissProt: P55210 Human
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别名
- active caspase 7 antibody
- Apoptotic protease Mch3 antibody
- CASP 7 antibody
see all
图片
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All lanes : Anti-Cleaved Caspase-7 antibody [EPR22840-25] (ab256469) at 1/1000 dilution
Lane 1 : Untreated HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : HeLa treated with 1 µM staurosporine for 3 hours whole cell lysate
Lane 3 : Untreated wild-type HAP1 whole cell lysate
Lane 4 : Wild-type HAP1 treated with 1 µM staurosporine for 3 hours whole cell lysate
Lane 5 : Untreated caspase-7 knockout HAP1 whole cell lysate
Lane 6 : Caspase-7 knockout HAP1 treated with 1 µM staurosporine for 3 hours whole cell lysate
Lane 7 : Mouse spleen lysate
Lane 8 : Rat spleen lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 34 kDa
Observed band size: 18,22 kDa why is the actual band size different from the predicted?
Exposure time: 26 secondsCaspase-7 is activated upon apoptotic induction. Caspase-7 p22 subunit (22 kDa) and p20 subunit (18 kDa) are observed. The molecular weight observed is consistent with what has been described in the literature (PMID: 16352606). This antibody reacts with an unidentifiable protein around 48 kDa. ab256469 was shown to specifically react with Caspase-7 in wild-type HAP1 cells as signal was lost in Caspase-7 knockout cells. Wild-type and Caspase-7 knockout samples were subjected to SDS-PAGE. ab256469 and ab181602 (Rabbit anti-GAPDH loading control) were incubated 1 hour at room temperature at 1/1000 dilution and 1/200,000 dilution respectively. Blots were developed with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) secondary antibody at 1/100,000 dilution for 1 hour at room temperature before imaging. The blot was developed on a BIO-RAD® ChemiDoc™ instrument using the ECL technique. Blocking and Diluting Buffer and concentration: 5% NFDM/TBST.
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Cleaved Caspase-7 was immunoprecipitated from 0.35 mg HeLa (Human cervix adenocarcinoma epithelial cell) treated with 1µM Staurosporine for 3h whole cell lysate 10µg with ab256469 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab256469. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/1000 dilution.
Lane 1: HeLa (Human cervix adenocarcinoma epithelial cell) treated with 1µM Staurosporine for 3h whole cell lysate 10µg
Lane 2: ab256469 IP in HeLa treated with 1µM Staurosporine for 3h whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab256469 in HeLa treated with 1µM Staurosporine for 3h whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 15 seconds.
This antibody reacts with an unidentifiable protein around 48 kDa.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (4)
ab256469 被引用在 4 文献中.
- Ma H & Ma Y Dapagliflozin Inhibits Ventricular Remodeling in Heart Failure Rats by Activating Autophagy through AMPK/mTOR Pathway. Comput Math Methods Med 2022:6260202 (2022). PubMed: 36193200
- Zhao X et al. Casein Kinase 2-Interacting Protein-1 Alleviates High Glucose-Reduced Autophagy, Oxidative Stress, and Apoptosis in Retinal Pigment Epithelial Cells via Activating the p62/KEAP1/NRF2 Signaling Pathway. J Ophthalmol 2021:6694050 (2021). PubMed: 33628480
- Shen H et al. Curcumin analog B14 has high bioavailability and enhances the effect of anti-breast cancer cells in vitro and in vivo. Cancer Sci 112:815-827 (2021). PubMed: 33316116
- Liu H et al. Effects of repetitive magnetic stimulation on motor function and GAP43 and 5-HT expression in rats with spinal cord injury. J Int Med Res 48:300060520970765 (2020). PubMed: 33356694