Key features and details
- Rabbit polyclonal to Cleaved Caspase-3
- Suitable for: WB
- Reacts with: Human, Recombinant fragment
- Isotype: IgG
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
参阅全部 Cleaved Caspase-3 一抗
描述兔多克隆抗体to Cleaved Caspase-3
经测试应用适用于: WBmore details
种属反应性与反应: Human, Recombinant fragment
- WB: HeLa (treated with staurosporine) whole cell lysate. Camptothecin treated Jurkat whole cell lysate. Cleaved Caspase 3 recombinant protein.
This product is manufactured by BioVision, an Abcam company and was previously called 3015 Anti-Caspase-3 (Active) Polyclonal Antibody.
Caspases are synthesized as inactive pro-enzymes that are processed to active form in cells undergoing apoptosis. Caspase 3 has been extensively studied and implicated to play an important role in apoptosis. Active caspase 3 proteolytically cleaves and activates other caspases, as well as relevant targets in the cells (e.g., PARP). This affinity purified antibody recognizing the active forms of caspase-3 provides a new tool for identifying apoptotic cell populations in both tissue sections and cultured cells.
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存放说明Shipped at 4°C. Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
存储溶液Preservative: 0.03% Proclin 300
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 0.5% BSA, 0.146% EDTA
Concentration information loading...
纯度Immunogen affinity purified
Primary antibody说明Caspases are synthesized as inactive pro-enzymes that are processed to active form in cells undergoing apoptosis. Caspase 3 has been extensively studied and implicated to play an important role in apoptosis. Active caspase 3 proteolytically cleaves and activates other caspases, as well as relevant targets in the cells (e.g., PARP). This affinity purified antibody recognizing the active forms of caspase-3 provides a new tool for identifying apoptotic cell populations in both tissue sections and cultured cells.
The Abpromise guarantee
1/50 - 1/500. Detects a band of approximately 17 kDa.
1/50 - 1/500. Detects a band of approximately 17 kDa.
功能Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-
-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin. Triggers cell adhesion in sympathetic neurons through RET cleavage.
组织特异性Highly expressed in lung, spleen, heart, liver and kidney. Moderate levels in brain and skeletal muscle, and low in testis. Also found in many cell lines, highest expression in cells of the immune system.
序列相似性Belongs to the peptidase C14A family.
翻译后修饰Cleavage by granzyme B, caspase-6, caspase-8 and caspase-10 generates the two active subunits. Additional processing of the propeptides is likely due to the autocatalytic activity of the activated protease. Active heterodimers between the small subunit of caspase-7 protease and the large subunit of caspase-3 also occur and vice versa.
S-nitrosylated on its catalytic site cysteine in unstimulated human cell lines and denitrosylated upon activation of the Fas apoptotic pathway, associated with an increase in intracellular caspase activity. Fas therefore activates caspase-3 not only by inducing the cleavage of the caspase zymogen to its active subunits, but also by stimulating the denitrosylation of its active site thiol.
- Information by UniProt
- Entrez Gene: 836 Human
- Omim: 600636 Human
- SwissProt: P42574 Human
- Unigene: 141125 Human
- active caspase 3 antibody
- Apopain antibody
- CASP 3 antibody
All lanes : Anti-Cleaved Caspase-3 antibody (ab2302)
Lane 1 : Untreated Jurkat cell line (human T cell leukemia cell line from peripheral blood)
Lane 2 : Camptothecin treated Jurkat cell line (human T cell leukemia cell line from peripheral blood)
Lysates/proteins at 60 µg per lane.
All lanes : Anti-Cleaved Caspase-3 antibody (ab2302) at 1 µg/ml
Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate (2 uM Staurosporine, 4Hr) at 20 µg
Lane 2 : HeLa whole cell lysate (untreated) at 20 µg
Lane 3 : Cleaved Caspase 3 (recombinant protein) at 0.1 µg
All lanes : 800CW Goat Anti-Rabbit IgG at 1/10000 dilution
Performed under reducing conditions.
Additional bands at: 17 kDa (possible mature (processed) protein), 24 kDa, 35 kDa. We are unsure as to the identity of these extra bands.
Anti-Cleaved Caspase-3 antibody (ab2302) at 1 µg/ml + Camptothecin
(2 µM) treated Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
ab2302 被引用在 1223 文献中.
- Wang S et al. Knockdown of circ_0004585 enhances the chemosensitivity of colorectal cancer cells to 5-fluorouracil via the miR-874-3p/CCND1 axis. Histol Histopathol 38:99-112 (2023). PubMed: 35900059
- Nian Y et al. Circ_0075960 targets the miR-202-5p/CTNND1 axis to promote the growth and migration of endometrial carcinoma cells via regulating Wnt/β-catenin signaling activity. J Gynecol Oncol 34:e11 (2023). PubMed: 36424704
- Wei Z et al. Inhibition of secretory leukocyte protease inhibitor (SLPI) promotes the PUMA-mediated apoptosis and chemosensitivity to cisplatin in colorectal cancer cells. Discov Oncol 14:1 (2023). PubMed: 36595102
- Liu F et al. LncRNA LIFR-AS1 overexpression suppressed the progression of serous ovarian carcinoma. J Clin Lab Anal N/A:e25470 (2022). WB ; Human . PubMed: 35778954
- Sánchez-Temprano A et al. Concurrent Akt, ERK1/2 and AMPK Activation by Obestatin Inhibits Apoptotic Signaling Cascades on Nutrient-Deprived PC12 Cells. Cell Mol Neurobiol 42:1607-1614 (2022). PubMed: 33400083