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Anti-Cleaved Caspase-3抗体(ab2302)

  • Datasheet
  • SDS
Reviews (58)Q&A (37)References (986)

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Western blot - Anti-Cleaved Caspase-3 antibody (ab2302)
  • Western blot - Anti-Cleaved Caspase-3 antibody (ab2302)
  • Western blot - Anti-Cleaved Caspase-3 antibody (ab2302)

Key features and details

  • Rabbit polyclonal to Cleaved Caspase-3
  • Suitable for: WB
  • Reacts with: Human, Recombinant fragment
  • Isotype: IgG

选择批间可重复性更高的重组抗体

Product image
Anti-Cleaved Caspase-3 antibody [E83-77] (ab32042)
  • 研究可靠 —— 各批次间结果一致且可重复
  • 长期批量供应 —— 采用重组技术,可实现快速生产
  • 首次实验即可成功 —— 经过大量验证确认了特异性
  • 符合伦理标准 —— 产品不含动物成分

概述

  • 产品名称

    Anti-Cleaved Caspase-3抗体
    参阅全部 Cleaved Caspase-3 一抗
  • 描述

    兔多克隆抗体to Cleaved Caspase-3
  • 宿主

    Rabbit
  • 经测试应用

    适用于: WBmore details
  • 种属反应性

    与反应: Human, Recombinant fragment
  • 免疫原

    Synthetic peptide corresponding to Human Cleaved Caspase-3. Synthetic peptide mapping to the N-terminus adjacent to the cleavage site of human caspase-3
    Database link: P42574
    (Peptide available as ab38283)

  • 阳性对照

    • WB: HeLa (treated with staurosporine) whole cell lysate. Camptothecin treated Jurkat whole cell lysate. Cleaved Caspase 3 recombinant protein.
  • 常规说明

    This product is manufactured by BioVision, an Abcam company and was previously called 3015 Anti-Caspase-3 (Active) Polyclonal Antibody.

    Caspases are synthesized as inactive pro-enzymes that are processed to active form in cells undergoing apoptosis. Caspase 3 has been extensively studied and implicated to play an important role in apoptosis. Active caspase 3 proteolytically cleaves and activates other caspases, as well as relevant targets in the cells (e.g., PARP). This affinity purified antibody recognizing the active forms of caspase-3 provides a new tool for identifying apoptotic cell populations in both tissue sections and cultured cells.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • 存储溶液

    Preservative: 0.03% Proclin 300
    Constituents: PBS, 30% Glycerol (glycerin, glycerine), 0.5% BSA, 0.146% EDTA
  • Concentration information loading...
  • 纯度

    Immunogen affinity purified
  • Primary antibody说明

    Caspases are synthesized as inactive pro-enzymes that are processed to active form in cells undergoing apoptosis. Caspase 3 has been extensively studied and implicated to play an important role in apoptosis. Active caspase 3 proteolytically cleaves and activates other caspases, as well as relevant targets in the cells (e.g., PARP). This affinity purified antibody recognizing the active forms of caspase-3 provides a new tool for identifying apoptotic cell populations in both tissue sections and cultured cells.
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Alzheimer's disease
    • Other
    • Cell Biology
    • Apoptosis
    • Intracellular
    • Caspases etc
    • Caspases
    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Other
    • Cancer
    • Invasion/microenvironment
    • Apoptosis
    • Caspases
    • Cancer
    • Cell Death
    • Apoptosis
    • Apoptosis Markers
    • Caspases

相关产品

  • Assay kits

    • Caspase-3 Assay Kit (Colorimetric) (ab39401)
    • Cleaved Caspase-3 Staining Kit (FITC) (ab65613)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant human Cleaved Caspase-3 protein (Active) (ab52101)
  • Related Products

    • Prestained Protein Ladder – Broad molecular weight (10-245 kDa) (ab116028)
    • Anti-Cytochrome C antibody [7H8.2C12] (ab13575)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab2302于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB (16)
1/50 - 1/500. Detects a band of approximately 17 kDa.
说明
WB
1/50 - 1/500. Detects a band of approximately 17 kDa.

靶标

  • 功能

    Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-
    -Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin. Triggers cell adhesion in sympathetic neurons through RET cleavage.
  • 组织特异性

    Highly expressed in lung, spleen, heart, liver and kidney. Moderate levels in brain and skeletal muscle, and low in testis. Also found in many cell lines, highest expression in cells of the immune system.
  • 序列相似性

    Belongs to the peptidase C14A family.
  • 翻译后修饰

    Cleavage by granzyme B, caspase-6, caspase-8 and caspase-10 generates the two active subunits. Additional processing of the propeptides is likely due to the autocatalytic activity of the activated protease. Active heterodimers between the small subunit of caspase-7 protease and the large subunit of caspase-3 also occur and vice versa.
    S-nitrosylated on its catalytic site cysteine in unstimulated human cell lines and denitrosylated upon activation of the Fas apoptotic pathway, associated with an increase in intracellular caspase activity. Fas therefore activates caspase-3 not only by inducing the cleavage of the caspase zymogen to its active subunits, but also by stimulating the denitrosylation of its active site thiol.
  • 细胞定位

    Cytoplasm.
  • Target information above from: UniProt accession P42574 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 836 Human
    • Omim: 600636 Human
    • SwissProt: P42574 Human
    • Unigene: 141125 Human
    • 别名

      • active caspase 3 antibody
      • Apopain antibody
      • CASP 3 antibody
      • CASP-3 antibody
      • CASP3 antibody
      • CASP3_HUMAN antibody
      • Caspase 3 antibody
      • Caspase-3 subunit p12 antibody
      • CPP 32 antibody
      • CPP-32 antibody
      • CPP32B antibody
      • Cysteine protease CPP32 antibody
      • PARP cleavage protease antibody
      • Protein Yama antibody
      • SCA-1 antibody
      • SCA1 antibody
      • SREBP cleavage activity 1 antibody
      • Yama antibody
      see all

    图片

    • Western blot - Anti-Cleaved Caspase-3 antibody (ab2302)
      Western blot - Anti-Cleaved Caspase-3 antibody (ab2302)
      All lanes : Anti-Cleaved Caspase-3 antibody (ab2302)

      Lane 1 : Untreated Jurkat cell line (human T cell leukemia cell line from peripheral blood)
      Lane 2 : Camptothecin treated Jurkat cell line (human T cell leukemia cell line from peripheral blood)

      Lysates/proteins at 60 µg per lane.
    • Western blot - Anti-Cleaved Caspase-3 antibody (ab2302)
      Western blot - Anti-Cleaved Caspase-3 antibody (ab2302)
      All lanes : Anti-Cleaved Caspase-3 antibody (ab2302) at 1 µg/ml

      Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate (2 uM Staurosporine, 4Hr) at 20 µg
      Lane 2 : HeLa whole cell lysate (untreated) at 20 µg
      Lane 3 : Cleaved Caspase 3 (recombinant protein) at 0.1 µg

      Secondary
      All lanes : 800CW Goat Anti-Rabbit IgG at 1/10000 dilution

      Performed under reducing conditions.

      Additional bands at: 17 kDa (possible mature (processed) protein), 24 kDa, 35 kDa. We are unsure as to the identity of these extra bands.

    • Western blot - Anti-Cleaved Caspase-3 antibody (ab2302)
      Western blot - Anti-Cleaved Caspase-3 antibody (ab2302)
      Anti-Cleaved Caspase-3 antibody (ab2302) at 1 µg/ml + Camptothecin
      (2 µM) treated Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate

    实验方案

    • Recommended protocol for WB with ab2302

    Click here to view the general protocols

    数据表及文件

    • SDS download

    • Datasheet download

      Download

    文献 (986)

    发表研究结果有使用 ab2302?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab2302 被引用在 986 文献中.

    • Liu F  et al. LncRNA LIFR-AS1 overexpression suppressed the progression of serous ovarian carcinoma. J Clin Lab Anal N/A:e25470 (2022). WB ; Human . PubMed: 35778954
    • Wang J  et al. MiR-122 Participates in Oxidative Stress and Apoptosis in STZ-Induced Pancreatic ß Cells by Regulating PI3K/AKT Signaling Pathway. Int J Endocrinol 2021:5525112 (2021). PubMed: 34054947
    • Shui S  et al. Non-enzymatic lipid peroxidation initiated by photodynamic therapy drives a distinct ferroptosis-like cell death pathway. Redox Biol 45:102056 (2021). PubMed: 34229160
    • Wu Q  et al. Histone deacetylase 1 facilitates aerobic glycolysis and growth of endometrial cancer. Oncol Lett 22:721 (2021). PubMed: 34429761
    • Zhou XH  et al. Osthole induces apoptosis of the HT-29 cells via endoplasmic reticulum stress and autophagy. Oncol Lett 22:726 (2021). PubMed: 34429766
    View all Publications for this product

    客户评价及客户问答

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    Sample
    Human Cell lysate - whole cell (Melanoma cells)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    20 µg
    Treatment
    Poly I:C
    Specification
    Melanoma cells
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
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    Immunocytochemistry/ Immunofluorescence abreview for Anti-Cleaved Caspase-3 antibody

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    Application
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    Sample
    Human Cell (Prostate cancer cells)
    Permeabilization
    Yes - Triton
    Specification
    Prostate cancer cells
    Blocking step
    Serum as blocking agent for 10 minute(s) · Concentration: 5% · Temperature: 22°C
    Fixative
    Paraformaldehyde
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    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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    提交于 Jun 22 2021

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    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (Lymph node)
    Permeabilization
    Yes - PBS/0.2 % Triton-X 100, 5 min RT
    Specification
    Lymph node
    Blocking step
    5 % goat-serum + 10 % BSA in PBS/0.1 % Tween20 as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Fixative
    Paraformaldehyde
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    Human Cell lysate - whole cell (HEK293T)
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    Reduced Denaturing
    Loading amount
    30 µg
    Specification
    HEK293T
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
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    Loading amount
    30 µg
    Specification
    heart
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
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    Immunocytochemistry/ Immunofluorescence abreview for Anti-Cleaved Caspase-3 antibody

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    Permeabilization
    No
    Specification
    HeLa cells
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 21°C
    Fixative
    Acetone
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    Immunohistochemistry (Frozen sections) abreview for Anti-Cleaved Caspase-3 antibody

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    Human Tissue sections (Melanoma metastasis)
    Specification
    Melanoma metastasis
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    Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
    Fixative
    Acetone
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    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Cleaved Caspase-3 antibody

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    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Brain)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate
    Permeabilization
    No
    Specification
    Brain
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
    Fixative
    Paraformaldehyde
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    Specification
    Dorsal root ganglia neurons
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 23°C
    Fixative
    Paraformaldehyde
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    Specification
    BRAIN
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Fixative
    FORMALIN
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