重组Anti-CLCA1抗体[EPR12254-88] (ab180851)
![Western blot - Anti-CLCA1 antibody [EPR12254-88] (ab180851)](https://www.abcam.cn/ps/products/180/ab180851/Images/ab180851-296795-anti-clca1-antibody-epr12254-88-western-blot.jpg "Western blot - Anti-CLCA1 antibody [EPR12254-88] (ab180851)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12254-88] to CLCA1
- Suitable for: Flow Cyt (Intra), IHC-P, WB, ICC/IF
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-CLCA1抗体[EPR12254-88]
参阅全部 CLCA1 一抗 -
描述
兔单克隆抗体[EPR12254-88] to CLCA1 -
宿主
Rabbit -
特异性
The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat. -
经测试应用
适用于: Flow Cyt (Intra), IHC-P, WB, ICC/IFmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Recombinant fragment within Human CLCA1. The exact sequence is proprietary.
Database link: A8K7I4 -
阳性对照
- Human colon cancer and human small intestine lysates; human colon tissue; A431 cells.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.21% BSA -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR12254-88 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab180851于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt (Intra) |
Use at an assay dependent concentration.
|
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| IHC-P | (1) |
1/4000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat. For unpurified use at 1/100 - 1/250. |
| WB | (1) |
1/1000 - 1/10000. Predicted molecular weight: 100 kDa.
|
| ICC/IF | (1) |
1/50 - 1/100.
|
| 说明 |
|---|
|
Flow Cyt (Intra)
Use at an assay dependent concentration. |
|
IHC-P
1/4000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat. For unpurified use at 1/100 - 1/250. |
|
WB
1/1000 - 1/10000. Predicted molecular weight: 100 kDa. |
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ICC/IF
1/50 - 1/100. |
靶标
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功能
May be involved in mediating calcium-activated chloride conductance. May play critical roles in goblet cell metaplasia, mucus hypersecretion, cystic fibrosis and AHR. May be involved in the regulation of mucus production and/or secretion by goblet cells. Involved in the regulation of tissue inflammation in the innate immune response. May play a role as a tumor suppressor. Induces MUC5AC. -
组织特异性
Highly expressed in small intestine and colon namely in intestinal basal crypt epithelia and goblet cells, and appendix. Weakly expressed in uterus, testis and kidney. Expressed in the airways epithelium of both asthmatic andhealthy patients. Expressed in the bronchial epithelium, especially in mucus-producing goblet cells. Expressed in normal turbinate mucosa and nasal polyp. Expressed in. -
序列相似性
Belongs to the CLCR family.
Contains 1 VWFA domain. -
翻译后修饰
Glycosylated.
The 125-kDa product is processed and yields to two cell-surface-associated subunits, a 90-kDa protein and a group of 37-to 41-kDa proteins. -
细胞定位
Secreted > extracellular space. Cell membrane. Protein that remains attached to the plasma membrane appeared to be predominantly localized to microvilli. - Information by UniProt
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数据库链接
- Entrez Gene: 1179 Human
- Entrez Gene: 23844 Mouse
- Entrez Gene: 308015 Rat
- Omim: 603906 Human
- SwissProt: A8K7I4 Human
- SwissProt: Q9D7Z6 Mouse
- Unigene: 194659 Human
- Unigene: 33483 Mouse
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别名
- CaCC antibody
- CaCC-1 antibody
- CACC1 antibody
see all
图片
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Western blot - Anti-CLCA1 antibody [EPR12254-88] (ab180851)Anti-CLCA1 antibody [EPR12254-88] (ab180851) at 1/10000 dilution (purified) + Human small intestine lysates at 15 µg
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution
Predicted band size: 100 kDaBlocking and diluting buffer: 5% NFDM/TBST
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![Flow Cytometry (Intracellular) - Anti-CLCA1 antibody [EPR12254-88] (ab180851)](https://www.abcam.cn/ps/products/180/ab180851/Images/ab180851-297993-anti-clca1-antibody-epr12254-88-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-CLCA1 antibody [EPR12254-88] (ab180851)Intracellular Flow Cytometry analysis of A431 (Human epidermoid carcinoma epithelial cell) cells labeling CLCA1 with purified ab180851 at 1/20 dilution (10µg/ml) (red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
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![Immunocytochemistry/ Immunofluorescence - Anti-CLCA1 antibody [EPR12254-88] (ab180851)](https://www.abcam.cn/ps/products/180/ab180851/Images/ab180851-297944-anti-clca1-antibody-epr12254-88-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-CLCA1 antibody [EPR12254-88] (ab180851)Immunocytochemistry/ Immunofluorescence analysis of SW480 (Human colorectal adenocarcinoma epithelial cell) cells labeling CLCA1 with Purified ab180851 at 1:100 dilution. Cells were fixed in 100% Methanol. ab150077 Goat anti rabbit IgG(Alexa Fluor® 488) was used as the secondary antibody at 1:1000 dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLCA1 antibody [EPR12254-88] (ab180851)](https://www.abcam.cn/ps/products/180/ab180851/Images/ab180851-297717-anti-clca1-antibody-epr12254-88-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLCA1 antibody [EPR12254-88] (ab180851)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human small intestine tissue sections labeling CLCA1 with Purified ab180851 at 1:4000 dilution (0.07 μg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.
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![Western blot - Anti-CLCA1 antibody [EPR12254-88] (ab180851)](https://www.abcam.cn/ps/products/180/ab180851/Images/ab180851-296796-anti-clca1-antibody-epr12254-88-western-blot.jpg)
Western blot - Anti-CLCA1 antibody [EPR12254-88] (ab180851)All lanes : Anti-CLCA1 antibody [EPR12254-88] (ab180851) at 1/50000 dilution (purified)
Lane 1 : Rat small intestine lysates
Lane 2 : Mouse small intestine lysates
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 100 kDaBlocking and diluting buffer: 5% NFDM/TBST
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![Immunocytochemistry/ Immunofluorescence - Anti-CLCA1 antibody [EPR12254-88] (ab180851)](https://www.abcam.cn/ps/products/180/ab180851/Images/ab180851-209680-anti-clca1-antibody-epr12254-88-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-CLCA1 antibody [EPR12254-88] (ab180851)Immunofluorescence analysis of A431 cells labeling CLCA1 using unpurified ab180851 at 1/50 dilution.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLCA1 antibody [EPR12254-88] (ab180851)](https://www.abcam.cn/ps/products/180/ab180851/Images/ab180851-209679-anti-clca1-antibody-epr12254-88-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLCA1 antibody [EPR12254-88] (ab180851)Immunohistochemical analysis of paraffin-embedded human colon tissue labeling CLCA1 using unpurified ab180851 at 1/100 dilution.
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![Western blot - Anti-CLCA1 antibody [EPR12254-88] (ab180851)](https://www.abcam.cn/ps/products/180/ab180851/Images/ab180851-209678-anti-clca1-antibody-epr12254-88-western-blot.jpg)
Western blot - Anti-CLCA1 antibody [EPR12254-88] (ab180851)All lanes : Anti-CLCA1 antibody [EPR12254-88] (ab180851) at 1/1000 dilution (unpurified)
Lane 1 : Human colon cancer lysates
Lane 2 : Human small intestine lysates
Lysates/proteins at 10 µg per lane.
Predicted band size: 100 kDa -
![Anti-CLCA1 antibody [EPR12254-88] (ab180851)](https://www.abcam.cn/ps/products/180/ab180851/Images/ab180851-9-benefits-of-recombinant-antibodies.png)
Anti-CLCA1 antibody [EPR12254-88] (ab180851)
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (17)
ab180851 被引用在 17 文献中.
- Hernandez JE et al. The arginine methyltransferase PRMT5 promotes mucosal defense in the intestine. Life Sci Alliance 6:N/A (2023). PubMed: 37666668
- Centeio R et al. TMEM16A/F support exocytosis but do not inhibit Notch-mediated goblet cell metaplasia of BCi-NS1.1 human airway epithelium. Front Physiol 14:1157704 (2023). PubMed: 37234411
- Zhao L et al. Mesenchymal-epithelial interaction regulates gastrointestinal tract development in mouse embryos. Cell Rep 40:111053 (2022). PubMed: 35830795
- Mahood TH et al. Integrating Proteomes for Lung Tissues and Lavage Reveals Pathways That Link Responses in Allergen-Challenged Mice. ACS Omega 6:1171-1189 (2021). PubMed: 33490776
- Centeio R et al. CLCA1 Regulates Airway Mucus Production and Ion Secretion Through TMEM16A. Int J Mol Sci 22:N/A (2021). PubMed: 34066250