Anti-ClC-4抗体(ab75008)
Key features and details
- Rabbit polyclonal to ClC-4
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-ClC-4抗体 -
描述
兔多克隆抗体to ClC-4 -
宿主
Rabbit -
经测试应用
适用于: WB, ICC/IFmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat
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免疫原
Synthetic peptide corresponding to Human ClC-4 (internal sequence).
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阳性对照
- WB: Extracts from MCF-7 cells ICC/IF: HUVEC cells
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+ -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
The antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab75008于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/500 - 1/1000. Predicted molecular weight: 85 kDa.
|
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| ICC/IF |
1/500 - 1/1000.
|
| 说明 |
|---|
|
WB
1/500 - 1/1000. Predicted molecular weight: 85 kDa. |
|
ICC/IF
1/500 - 1/1000. |
靶标
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功能
Proton-coupled chloride transporter. Functions as antiport system and exchanges chloride ions against protons. -
组织特异性
Abundant in skeletal muscle and also detectable in brain and heart. -
序列相似性
Belongs to the chloride channel (TC 2.A.49) family. ClC-4/CLCN4 subfamily.
Contains 2 CBS domains. -
细胞定位
Early endosome membrane. Late endosome membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 1183 Human
- Entrez Gene: 12727 Mouse
- Entrez Gene: 60586 Rat
- Omim: 302910 Human
- SwissProt: P51793 Human
- SwissProt: Q61418 Mouse
- SwissProt: P51794 Rat
- Unigene: 495674 Human
see all -
别名
- Chloride channel 4 antibody
- Chloride channel protein 4 antibody
- Chloride channel, voltage sensitive 4 antibody
see all
图片
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Western blot - Anti-ClC-4 antibody (ab75008)All lanes : Anti-ClC-4 antibody (ab75008) at 1/500 dilution
Lane 1 : extracts from MCF-7 cells
Lane 2 : extracts from MCF-7 cells with immunising peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size: 85 kDa
Observed band size: 85 kDa
Additional bands at: 100 kDa, 45 kDa. We are unsure as to the identity of these extra bands. -
Immunocytochemistry/ Immunofluorescence - Anti-ClC-4 antibody (ab75008)Immunofluorescence analysis of ClC-4 in HUVEC cells, using ab75008 at a 1/500 dilution with (right image) or without (left image) the immunising peptide.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab75008 被引用在 1 文献中.
- Wang C et al. Comprehensive analysis and experimental validation reveal elevated CLCN4 is a promising biomarker in endometrial cancer. Aging (Albany NY) 15:8744-8769 (2023). PubMed: 37671947