Anti-Claudin 7/CLDN-7抗体(ab27487)
Key features and details
- Rabbit polyclonal to Claudin 7/CLDN-7
- Suitable for: ICC, IHC-Fr, WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-Claudin 7/CLDN-7抗体
参阅全部 Claudin 7/CLDN-7 一抗 -
描述
兔多克隆抗体to Claudin 7/CLDN-7 -
宿主
Rabbit -
经测试应用
适用于: ICC, IHC-Fr, WB, IHC-P, ICC/IFmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide within Human Claudin 7/CLDN-7 aa 150 to the C-terminus (C terminal). The exact sequence is proprietary.
Database link: O95471 -
常规说明
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
Ab27487 is affinity purified. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab27487于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC | (1) |
Use at an assay dependent concentration.
|
| IHC-Fr | (3) |
Use at an assay dependent concentration.
|
| WB | (5) |
Use at an assay dependent concentration. Predicted molecular weight: 22 kDa.
|
| IHC-P | (3) |
1/200. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. In particular, boil tissue sections in 10mM citrate buffer pH 6.0 for 10 min then cool at room temperature for 20 min.
|
| ICC/IF |
Use at an assay dependent concentration. PubMed: 21525338
|
| 说明 |
|---|
|
ICC
Use at an assay dependent concentration. |
|
IHC-Fr
Use at an assay dependent concentration. |
|
WB
Use at an assay dependent concentration. Predicted molecular weight: 22 kDa. |
|
IHC-P
1/200. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. In particular, boil tissue sections in 10mM citrate buffer pH 6.0 for 10 min then cool at room temperature for 20 min. |
|
ICC/IF
Use at an assay dependent concentration. PubMed: 21525338 |
靶标
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功能
Plays a major role in tight junction-specific obliteration of the intercellular space. -
组织特异性
Expressed in kidney, lung and prostate. Isoform 1 seems to be predominant, except in some normal prostate samples, where isoform 2 is the major form. Down-regulated in breast cancers, including ductal carcinoma in situ (DCIS), lobular carcinoma in situ (LCIS) and invasive ductal carcinoma (IDC) (at protein level), as well as in several cancer cell lines. Loss of expression correlates with histological grade, occurring predominantly in high-grade lesions. -
序列相似性
Belongs to the claudin family. -
翻译后修饰
Phosphorylated. -
细胞定位
Cell membrane. Lateral cell membrane. Cell junction > tight junction. Co-localizes with EPCAM at the lateral cell membrane and tight junction. - Information by UniProt
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数据库链接
- Entrez Gene: 1366 Human
- Entrez Gene: 53624 Mouse
- Entrez Gene: 65132 Rat
- Omim: 609131 Human
- SwissProt: O95471 Human
- SwissProt: Q9Z261 Mouse
- SwissProt: Q9Z1L1 Rat
- Unigene: 513915 Human
see all -
别名
- CEPTR L2 antibody
- CEPTRL 2 antibody
- CEPTRL2 antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin 7/CLDN-7 antibody (ab27487)
Ab27487, at a dilution of 1/200, staining Claudin 7/CLDN-7 in formalin fixed paraffin embedded human colon carcinoma tissue section by Immunohistochemistry.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (40)
ab27487 被引用在 40 文献中.
- Xu L et al. Digital Image Analysis-Based Evaluation of Claudin-1 and Claudin-7 Delocalization in Cutaneous Squamous Cell Carcinoma and in Its Precancerous State. J Oncol 2022:2750193 (2022). PubMed: 35432533
- Wu J et al. Limosilactobacillus reuteri SLZX19-12 Protects the Colon from Infection by Enhancing Stability of the Gut Microbiota and Barrier Integrity and Reducing Inflammation. Microbiol Spectr 10:e0212421 (2022). PubMed: 35658572
- Ueda M et al. Aeromonas sobria Serine Protease Degrades Several Protein Components of Tight Junctions and Assists Bacterial Translocation Across the T84 Monolayer. Front Cell Infect Microbiol 12:824547 (2022). PubMed: 35273923
- Wang K et al. Cldn-7 deficiency promotes experimental colitis and associated carcinogenesis by regulating intestinal epithelial integrity. Oncoimmunology 10:1923910 (2021). PubMed: 34026335
- Schaefer J et al. Uterine kisspeptin receptor critically regulates epithelial estrogen receptor α transcriptional activity at the time of embryo implantation in a mouse model. Mol Hum Reprod 27:N/A (2021). PubMed: 34524460