Name: Anti-Claudin 5 antibody [EPR7583]
Brand: RabMAb
SKU: ab131259
Rating: 5 (7 reviews)

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Immunocytochemistry/ Immunofluorescence - Anti-Claudin 5 antibody [EPR7583] (ab131259)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR7583] to Claudin 5
Suitable for: ICC/IF, WB, IHC-P
Reacts with: Human

Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 568 Alexa Fluor® 594 Alexa Fluor® 647 Alexa Fluor® 750 Carrier Free

产品名称

Anti-Claudin 5抗体[EPR7583]
参阅全部 Claudin 5 一抗
描述

兔单克隆抗体[EPR7583] to Claudin 5
宿主

Rabbit
特异性

ab131259 failed to detect target band in mouse brain tissue. This antibody is unsuitable to be used in IHC on mouse tissues.

This antibody failed to get signal in cell lines when performing western blot, so we do not recommend to use it when testing cell lines in westen blot.
经测试应用

适用于: ICC/IF, WB, IHC-Pmore details
不适用于: IHC-Fr
种属反应性

与反应: Human
不与反应: Mouse
免疫原

Synthetic peptide within Human Claudin 5 aa 200 to the C-terminus (intracellular). The exact sequence is proprietary.
Database link: O00501
阳性对照
- WB: Human fetal brain, lung squamous cell cancer and small cell lung cancer, IHC-P: Human colon, tonsil, placenta, uterus, kidney and lung tissues. ICC/IF: HUVEC cells.
常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

形式

Liquid
存放说明

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
存储溶液

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
纯度

Protein A purified
克隆

单克隆
克隆编号

EPR7583
同种型

IgG
研究领域
- Neuroscience
- Processes

Alternative Versions
Compatible Secondaries
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
- Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)

The Abpromise guarantee

Abpromise™承诺保证使用ab131259于以下的经测试应用

“应用说明”部分下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用	Ab评论	说明
ICC/IF	(1)	Use a concentration of 0.008 µg/ml.
WB	(1)	1/1000 - 1/10000. Detects a band of approximately 23 kDa (predicted molecular weight: 23 kDa). ab131259 failed to detect target band in mouse brain tissue. This antibody failed to get signal in cell lines when performing western blot, so we do not recommend to use it when testing cell lines in westen blot.
IHC-P	(4)	1/1000 - 1/10000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

说明
ICC/IF Use a concentration of 0.008 µg/ml.
WB 1/1000 - 1/10000. Detects a band of approximately 23 kDa (predicted molecular weight: 23 kDa). ab131259 failed to detect target band in mouse brain tissue. This antibody failed to get signal in cell lines when performing western blot, so we do not recommend to use it when testing cell lines in westen blot.
IHC-P 1/1000 - 1/10000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

应用说明

Is unsuitable for IHC-Fr.

功能

Plays a major role in tight junction-specific obliteration of the intercellular space.
序列相似性

Belongs to the claudin family.
细胞定位

Cell junction > tight junction. Cell membrane.
Target information above from: UniProt accession O00501 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
数据库链接
- Entrez Gene: 7122 Human
- Omim: 602101 Human
- SwissProt: O00501 Human
- Unigene: 505337 Human
别名
- Androgen withdrawal and apoptosis induced protein RVP1 like antibody
- AWAL antibody
- BEC 1 antibody
- BEC1 antibody
- Claudin 5 (transmembrane protein deleted in velocardiofacial syndrome) antibody
- Claudin-5 antibody
- Claudin5 antibody
- CLD5_HUMAN antibody
- CLDN 5 antibody
- Cldn5 antibody
- CPETR L1 antibody
- CPETRL 1 antibody
- CPETRL1 antibody
- TMDVCF antibody
- TMVCF antibody
- Transmembrane protein deleted in VCFS antibody
- Transmembrane protein deleted in velocardiofacial syndrome antibody
see all

Immunocytochemistry/ Immunofluorescence - Anti-Claudin 5 antibody [EPR7583] (ab131259)

ab131259 staining Claudin 5 in HUV-EC cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab131259 at 0.008µg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor^® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor^® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue). Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.
Western blot - Anti-Claudin 5 antibody [EPR7583] (ab131259)

All lanes : Anti-Claudin 5 antibody [EPR7583] (ab131259) at 1/1000 dilution (unpurified)

Lane 1 : Human fetal brain lysate
Lane 2 : Human lung squamous cell cancer lysate
Lane 3 : Human small cell lung cancer lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP labelled Goat anti Rabbit IgG at 1/2000 dilution

Predicted band size: 23 kDa
Western blot - Anti-Claudin 5 antibody [EPR7583] (ab131259)

Anti-Claudin 5 antibody [EPR7583] (ab131259) at 1/5000 dilution (purified) + Human small cell lung cancer at 10 µg

Secondary
HRP goat anti-rabbit IgG (H+L) at 1/5000 dilution

Predicted band size: 23 kDa
Observed band size: 20 kDa why is the actual band size different from the predicted?

Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
Western blot - Anti-Claudin 5 antibody [EPR7583] (ab131259)

Anti-Claudin 5 antibody [EPR7583] (ab131259) at 1/20000 dilution (purified) + Human lung squamous cell cancer lysate at 10 µg

Secondary
HRP goat anti-rabbit IgG (H+L) at 1/5000 dilution

Predicted band size: 23 kDa
Observed band size: 20 kDa why is the actual band size different from the predicted?

Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin 5 antibody [EPR7583] (ab131259)

Immunohistochemical staining of paraffin embedded human colon with purified ab131259 at a working dilution of 1/1000. The secondary antibody used is HRP goat anti-rabbit IgG H&L (ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin 5 antibody [EPR7583] (ab131259)

Immunohistochemical analysis of paraffin embedded Human kidney tissue labelling Claudin 5 with unpurified ab131259 antibody at 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin 5 antibody [EPR7583] (ab131259)

Immunohistochemical analysis of paraffin embedded Human lung tissue labelling Claudin 5 with unpurified ab131259 antibody at 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin 5 antibody [EPR7583] (ab131259)

Unpurified ab131259 showing positive staining in Normal tonsil tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin 5 antibody [EPR7583] (ab131259)

Unpurified ab131259 showing positive staining in Normal placenta tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin 5 antibody [EPR7583] (ab131259)

Unpurified ab131259 showing positive staining in Normal uterus tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin 5 antibody [EPR7583] (ab131259)

Unpurified ab131259 showing positive staining in Normal colon tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Anti-Claudin 5 antibody [EPR7583] (ab131259)

Immunohistochemistry protocols
Western blot protocols

Click here to view the general protocols

SDS download

Country/Region

Language
Datasheet download

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发表研究结果有使用 ab131259？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab131259 被引用在 90 文献中.

Lin L et al. Mito-TEMPO, a Mitochondria-Targeted Antioxidant, Improves Cognitive Dysfunction due to Hypoglycemia: an Association with Reduced Pericyte Loss and Blood-Brain Barrier Leakage. Mol Neurobiol 60:672-686 (2023). PubMed: 36357613
Pesti A et al. Liver alterations and detection of SARS-CoV-2 RNA and proteins in COVID-19 autopsies. Geroscience 45:1015-1031 (2023). PubMed: 36527584
Wu Y et al. P7C3-A20 Attenuates Microglial Inflammation and Brain Injury after ICH through Activating the NAD+/Sirt3 Pathway. Oxid Med Cell Longev 2023:7857760 (2023). PubMed: 36819779
Huang P et al. YTHDF1 Attenuates TBI-Induced Brain-Gut Axis Dysfunction in Mice. Int J Mol Sci 24:N/A (2023). PubMed: 36835655
Nakayama-Kitamura K et al. Usefulness of a humanized tricellular static transwell blood-brain barrier model as a microphysiological system for drug development applications. - A case study based on the benchmark evaluations of blood-brain barrier microphysiological system. Regen Ther 22:192-202 (2023). PubMed: 36891355

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Application

Immunocytochemistry/ Immunofluorescence

Sample

Human Cell (organoid)

Permeabilization

Yes - 0.5%tritonX-100

Specification

organoid

Blocking step

BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Fixative

Paraformaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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提交于 Nov 26 2021

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Key features and details

Related conjugates and formulations

概述

产品名称

描述

宿主

特异性

经测试应用

种属反应性

免疫原

阳性对照

常规说明

性能

形式

存放说明

存储溶液

纯度

克隆

克隆编号

同种型

研究领域

相关产品

Alternative Versions

Compatible Secondaries

Isotype control

应用

The Abpromise guarantee

靶标

功能

序列相似性

细胞定位

数据库链接

别名

图片

实验方案

数据表及文件

SDS download

Datasheet download

文献 (90)

客户评价及客户问答

Immunocytochemistry/ Immunofluorescence abreview for Anti-Claudin 5 antibody [EPR7583]