Anti-Claudin 3抗体(ab52231)
Key features and details
- Rabbit polyclonal to Claudin 3
- Suitable for: ELISA, IP, WB, ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-Claudin 3抗体
参阅全部 Claudin 3 一抗 -
描述
兔多克隆抗体to Claudin 3 -
宿主
Rabbit -
经测试应用
适用于: ELISA, IP, WB, ICC/IF, IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide derived from within amino acids 181 and 220 of Human Claudin-3
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 0.87% Sodium chloride, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab52231于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ELISA |
Use at an assay dependent concentration.
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| IP |
Use at an assay dependent concentration.
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| WB | (5) |
1/500 - 1/1000. Detects a band of approximately 23 kDa (predicted molecular weight: 23 kDa).
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| ICC/IF |
Use a concentration of 5 µg/ml.
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| IHC-P |
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| 说明 |
|---|
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ELISA
Use at an assay dependent concentration. |
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IP
Use at an assay dependent concentration. |
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WB
1/500 - 1/1000. Detects a band of approximately 23 kDa (predicted molecular weight: 23 kDa). |
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ICC/IF
Use a concentration of 5 µg/ml. |
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IHC-P
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
Plays a major role in tight junction-specific obliteration of the intercellular space, through calcium-independent cell-adhesion activity. -
疾病相关
Note=CLDN3 is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region. -
序列相似性
Belongs to the claudin family. -
细胞定位
Cell junction > tight junction. Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 1365 Human
- Omim: 602910 Human
- SwissProt: O15551 Human
- Unigene: 647023 Human
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别名
- C7orf1 antibody
- Claudin-3 antibody
- Claudin3 antibody
see all
图片
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Western blot - Anti-Claudin 3 antibody (ab52231)All lanes : Anti-Claudin 3 antibody (ab52231) at 1/500 dilution
Lane 1 : Extracts from HuvEc cells, untreated.
Lane 2 : Extracts from HuvEc cells, treated with the immunizing peptide.
Predicted band size: 23 kDa
Observed band size: 23 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin 3 antibody (ab52231)IHC image of ab52231 staining in normal human colon formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab52231, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times. -
Immunocytochemistry/ Immunofluorescence - Anti-Claudin 3 antibody (ab52231)ICC/IF image of ab52231 stained MCF7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab52231, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h.Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. -
Western blot - Anti-Claudin 3 antibody (ab52231)This image is courtesy of an anonymous AbreviewAll lanes : Anti-Claudin 3 antibody (ab52231)
Lane 1 : Human urothelial cells (differentiated)
Lane 2 : Human urothelial cells (non-differentiated)
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : IRDye® 800-conjugated Goat anti-rabbit IgG polyclonal
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 23 kDa
Exposure time: 2 minutes
Blocked with 0.1% Milk for 1 hour at 22°C
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (7)
ab52231 被引用在 7 文献中.
- Xia X et al. Adverse effect of assisted reproductive technology-related hyperoestrogensim on the secretion and absorption of uterine fluid in superovulating mice during the peri-implantation period. Front Endocrinol (Lausanne) 14:859204 (2023). PubMed: 36950692
- Li Y et al. Role of Autophagy Inducers and Inhibitors in Intestinal Barrier Injury Induced by Intestinal Ischemia-Reperfusion (I/R). J Immunol Res 2022:9822157 (2022). PubMed: 35942211
- Yuan M et al. ZDHHC12-mediated claudin-3 S-palmitoylation determines ovarian cancer progression. Acta Pharm Sin B 10:1426-1439 (2020). PubMed: 32963941
- Vancamelbeke M et al. Butyrate does not protect against inflammation-induced loss of epithelial barrier function and cytokine production in primary cell monolayers from patients with ulcerative colitis. J Crohns Colitis N/A:N/A (2019). PubMed: 30919886
- Miranda J et al. Syncytiotrophoblast of Placentae from Women with Zika Virus Infection Has Altered Tight Junction Protein Expression and Increased Paracellular Permeability. Cells 8:N/A (2019). PubMed: 31569528
- Han CL et al. An Informatics-assisted Label-free Approach for Personalized Tissue Membrane Proteomics: Case Study on Colorectal Cancer. Mol Cell Proteomics 10:M110.003087 (2011). WB ; Human . PubMed: 21209152
- Ye P et al. CD24 regulated gene expression and distribution of tight junction proteins is associated with altered barrier function in oral epithelial monolayers. BMC Cell Biol 10:2 (2009). PubMed: 19138432