重组Anti-Claudin 2抗体[EPR28730-220] (ab317317)
![Western blot - Anti-Claudin 2 antibody [EPR28730-220] (ab317317)](https://www.abcam.cn/ps/products/317/ab317317/Images/ab317317-539764-anti-claudin-2-antibody-epr28730-220-western-blot-lovo-human-colorectal-adenocarcinoma-epithelial-cell-whole-cell-lysate-20-ug.jpg "Western blot - Anti-Claudin 2 antibody [EPR28730-220] (ab317317)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR28730-220] to Claudin 2
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-Claudin 2抗体[EPR28730-220]
参阅全部 Claudin 2 一抗 -
描述
兔单克隆抗体[EPR28730-220] to Claudin 2 -
宿主
Rabbit -
特异性
Not suitable for use with tissue lysates in WB. A non-specific band at ~25KDa is observed.
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经测试应用
适用于: WBmore details
不适用于: Flow Cyt (Intra),ICC/IF,IHC-P or IP -
种属反应性
与反应: Human
不与反应: Mouse, Rat -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: LoVo, HT-29, HCT-15 and A549 lysates.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR28730-220 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab317317于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/1000.
|
| 说明 |
|---|
|
WB
1/1000. |
靶标
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功能
Plays a major role in tight junction-specific obliteration of the intercellular space, through calcium-independent cell-adhesion activity. -
序列相似性
Belongs to the claudin family. -
细胞定位
Cell junction > tight junction. Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 9075 Human
- Omim: 300520 Human
- SwissProt: P57739 Human
- Unigene: 522746 Human
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别名
- Claudin-2 antibody
- Claudin2 antibody
- CLD2_HUMAN antibody
see all
图片
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Western blot - Anti-Claudin 2 antibody [EPR28730-220] (ab317317)All lanes : Anti-Claudin 2 antibody [EPR28730-220] (ab317317) at 1/1000 dilution
Lane 1 : LoVo (human colorectal adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : HT-29 (human colorectal adenocarcinoma epithelial cell) whole cell lysate
Lane 3 : HCT-15 (human colon epithelial cell) whole cell lysate
Lane 4 : SW620 (human colorectal adenocarcinoma epithelial cell) whole cell lysate
Lane 5 : SW480 (human colorectal adenocarcinoma epithelial cell) whole cell lysate
Lane 6 : HCT 116 (human colorectal carcinoma epithelial cell) whole cell lysate
Lane 7 : A549 (human lung carcinoma epithelial cell) whole cell lysate
Lane 8 : PANC-1 (human pancreatic epithelioid carcinoma epithelial cell) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Observed band size: 20 kDa why is the actual band size different from the predicted?
Exposure time: 81 secondsBlocking and diluting buffer and concentration: 5% NFDM/TBST.
Low expression: SW620, SW480, HCT 116 (PMID: 21383692), PANC-1.
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 21383692).
The identity of the higher MW bands at approximately 60 kDa and 110 kDa are unknown.
The identity of the lower MW band at approximately 10 kDa in lane 1 is unknown.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.
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![Anti-Claudin 2 antibody [EPR28730-220] (ab317317)](https://www.abcam.cn/ps/products/317/ab317317/Images/ab317317-1-benefitsofrecombinantantibodies.jpg)
Anti-Claudin 2 antibody [EPR28730-220] (ab317317)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (0)
ab317317 尚未被引用在任何文献中。