Anti-Claudin 2抗体(ab53032)
Key features and details
- Rabbit polyclonal to Claudin 2
- Suitable for: ICC/IF, ELISA, IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-Claudin 2抗体
参阅全部 Claudin 2 一抗 -
描述
兔多克隆抗体to Claudin 2 -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, ELISA, IHC-P, WBmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Rat
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免疫原
A synthetic peptide derived from human Claudin 2.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS
Without Mg+2 and Ca+2 -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
ab53032 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab53032于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF | (1) |
Use at an assay dependent concentration.
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| ELISA |
Use at an assay dependent concentration.
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| IHC-P | (1) |
Use at an assay dependent concentration.
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| WB |
1/500 - 1/1000. Detects a band of approximately 25 kDa (predicted molecular weight: 25 kDa).
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| 说明 |
|---|
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ICC/IF
Use at an assay dependent concentration. |
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ELISA
Use at an assay dependent concentration. |
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IHC-P
Use at an assay dependent concentration. |
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WB
1/500 - 1/1000. Detects a band of approximately 25 kDa (predicted molecular weight: 25 kDa). |
靶标
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功能
Plays a major role in tight junction-specific obliteration of the intercellular space, through calcium-independent cell-adhesion activity. -
序列相似性
Belongs to the claudin family. -
细胞定位
Cell junction > tight junction. Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 9075 Human
- Entrez Gene: 12738 Mouse
- Entrez Gene: 300920 Rat
- Omim: 300520 Human
- SwissProt: P57739 Human
- SwissProt: O88552 Mouse
- Unigene: 522746 Human
- Unigene: 117068 Mouse
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别名
- Claudin-2 antibody
- Claudin2 antibody
- CLD2_HUMAN antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin 2 antibody (ab53032)
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using ab53032. The picture on the right is blocked with the synthesized peptide.
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Western blot - Anti-Claudin 2 antibody (ab53032)All lanes : Anti-Claudin 2 antibody (ab53032)
Lane 1 : NIH/3T3 cells
Lane 2 : NIH/3T3 cells with blocking peptide
Predicted band size: 25 kDa
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (54)
ab53032 被引用在 54 文献中.
- Xiang S & Xiao J Protective effects of syringic acid on inflammation, apoptosis and intestinal barrier function in Caco-2 cells following oxygen-glucose deprivation/reoxygenation-induced injury. Exp Ther Med 23:66 (2022). PubMed: 34934437
- Brice DP et al. Interleukin-27 Regulates the Function of the Gastrointestinal Epithelial Barrier in a Human Tissue-Derived Organoid Model. Biology (Basel) 11:N/A (2022). PubMed: 35336801
- Wang R et al. Gut microbiota regulates acute myeloid leukaemia via alteration of intestinal barrier function mediated by butyrate. Nat Commun 13:2522 (2022). PubMed: 35534496
- Reiner J et al. The dual GLP-1 and GLP-2 receptor agonist dapiglutide promotes barrier function in murine short bowel. Ann N Y Acad Sci 1514:132-141 (2022). PubMed: 35580981
- He L et al. Glutamine in suppression of lipopolysaccharide-induced piglet intestinal inflammation: The crosstalk between AMPK activation and mitochondrial function. Anim Nutr 10:137-147 (2022). PubMed: 35663373