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重组RabMAb

重组Anti-Claudin 1抗体[SP128] - BSA and Azide free (ab271884)

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Flow Cytometry - Anti-Claudin 1 antibody [SP128] - BSA and Azide free (ab271884)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin 1 antibody [SP128] - BSA and Azide free (ab271884)
  • Anti-Claudin 1 antibody [SP128] - BSA and Azide free (ab271884)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [SP128] to Claudin 1 - BSA and Azide free
  • Suitable for: WB, Flow Cyt, IHC-P
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Unconjugated

概述

  • 产品名称

    Anti-Claudin 1抗体[SP128] - BSA and Azide free
    参阅全部 Claudin 1 一抗

  • 描述

    兔单克隆抗体[SP128] to Claudin 1 - BSA and Azide free

  • 宿主

    Rabbit

  • 经测试应用

    适用于: WB, Flow Cyt, IHC-Pmore details

  • 种属反应性

    与反应: Human
    预测可用于: Cow, Pig不与反应: Mouse, Rat

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • Flow cyt: HepG2 cells. IHC-P: Human liver carcinoma tissue.

  • 常规说明

    ab271884 is the carrier-free version of ab115783.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • 存储溶液

    pH: 7.20
    Constituent: PBS

  • 无载体

    是
  • Concentration information loading...

  • 纯度

    Protein A/G purified

  • 克隆

    单克隆

  • 克隆编号

    SP128

  • 同种型

    IgG

  • 研究领域

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab271884于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB
Use at an assay dependent concentration. Predicted molecular weight: 22 kDa.
Flow Cyt
Use at an assay dependent concentration.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
IHC-P
Use at an assay dependent concentration.
说明
WB
Use at an assay dependent concentration. Predicted molecular weight: 22 kDa.
Flow Cyt
Use at an assay dependent concentration.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
IHC-P
Use at an assay dependent concentration.

靶标

  • 功能

    Claudins function as major constituents of the tight junction complexes that regulate the permeability of epithelia. While some claudin family members play essential roles in the formation of impermeable barriers, others mediate the permeability to ions and small molecules. Often, several claudin family members are coexpressed and interact with each other, and this determines the overall permeability. CLDN1 is required to prevent the paracellular diffusion of small molecules through tight junctions in the epidermis and is required for the normal barrier function of the skin. Required for normal water homeostasis and to prevent excessive water loss through the skin, probably via an indirect effect on the expression levels of other proteins, since CLDN1 itself seems to be dispensable for water barrier formation in keratinocyte tight junctions (PubMed:23407391).
    (Microbial infection) Acts as a receptor for hepatitis C virus in hepatocytes (PubMed:17325668). Acts as a receptor for dengue virus (PubMed:24074594).

  • 组织特异性

    Strongly expressed in liver and kidney. Expressed in heart, brain, spleen, lung and testis.

  • 疾病相关

    Ichthyosis-sclerosing cholangitis neonatal syndrome

  • 序列相似性

    Belongs to the claudin family.

  • 细胞定位

    Cell junction, tight junction. Cell membrane.
  • Information by UniProt

  • 数据库链接

    • 别名

      • Claudin-1 antibody
      • Claudin1 antibody
      • CLD 1 antibody
      • CLD1 antibody
      • CLD1_HUMAN antibody
      • CLDN 1 antibody
      • Cldn1 antibody
      • ILVASC antibody
      • SEMP 1 antibody
      • SEMP1 antibody
      • Senescence associated epithelial membrane protein 1 antibody
      • Senescence associated epithelial membrane protein antibody
      • Senescence-associated epithelial membrane protein antibody
      see all

    图片

    • Flow Cytometry - Anti-Claudin 1 antibody [SP128] - BSA and Azide free (ab271884)
      Flow Cytometry - Anti-Claudin 1 antibody [SP128] - BSA and Azide free (ab271884)

      Flow cytometric analysis of rabbit anti-Claudin 1 (SP128) antibody ab115783 (1/100) in HepG2 cells (green) compared to negative control of rabbit IgG (blue).

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab115783).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin 1 antibody [SP128] - BSA and Azide free (ab271884)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin 1 antibody [SP128] - BSA and Azide free (ab271884)

      Staining of Human Claudin 1 in a Formalin/PFA-fixed paraffin-embedded section of Human Liver Carcinoma using ab115783 at a dilution of 1/200.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab115783).

    • Anti-Claudin 1 antibody [SP128] - BSA and Azide free (ab271884)
      Anti-Claudin 1 antibody [SP128] - BSA and Azide free (ab271884)

    实验方案

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    文献 (0)

    发表研究结果有使用 ab271884？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab271884 尚未被引用在任何文献中。

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com