Claudin 1重组抗体[RM1186]_Claudin 1抗体(ab317709)| Abcam中文官网

产品名称

Anti-Claudin 1抗体[RM1186]
参阅全部 Claudin 1 一抗
描述

兔重组multiclonal [RM1186] to Claudin 1
宿主

Rabbit
特异性

Unsuitable for mouse ICC and mouse FC.
经测试应用

适用于: ICC/IF, IP, WB, IHC-P, Flow Cyt (Intra)more details
不适用于: IHC-Fr
种属反应性

与反应: Mouse, Rat, Human
免疫原

This product was produced with the following immunogens:
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
阳性对照
- WB: A549, HepG2, T-47D, HT-29, Wide-type A431, CLDN1 knockout A431 whole cell lysate, Mouse colon, Mouse liver, Rat colon, Rat liver and NMuLi lysates. IHC-P: Human colon, Human liver, Human gastric adenocarcinoma, Mouse colon, Mouse liver, Mouse breast carcinoma and Rat colon. ICC/IF: CLDN1 KO A431 cells. Flow Cyt: CLDN1 KO A431 cell. IP: Mouse colon, NMuLi, A549 and Rat liver cells.
常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

形式

Liquid
存放说明

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
存储溶液

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA
Concentration information loading...
纯度

Protein A purified
克隆

Recombinant Multiclonal
克隆编号

RM1186
同种型

IgG
研究领域

Alternative Versions
Compatible Secondaries
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
KO cell lines
- Human CLDN1 knockout A-431 cell line (ab261889)
KO cell lysates
- Human CLDN1 knockout A-431 cell lysate (ab261698)
Related Products
- Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602)
- Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (ab195889)

The Abpromise guarantee

Abpromise™承诺保证使用ab317709于以下的经测试应用

“应用说明”部分下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用	Ab评论	说明
ICC/IF		1/100.
IP		1/1000.
WB		1/1000. Predicted molecular weight: 22 kDa.
IHC-P		1/100 - 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Flow Cyt (Intra)		1/500.

说明
ICC/IF 1/100.
IP 1/1000.
WB 1/1000. Predicted molecular weight: 22 kDa.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Flow Cyt (Intra) 1/500.

应用说明

Is unsuitable for IHC-Fr.

功能

Claudins function as major constituents of the tight junction complexes that regulate the permeability of epithelia. While some claudin family members play essential roles in the formation of impermeable barriers, others mediate the permeability to ions and small molecules. Often, several claudin family members are coexpressed and interact with each other, and this determines the overall permeability. CLDN1 is required to prevent the paracellular diffusion of small molecules through tight junctions in the epidermis and is required for the normal barrier function of the skin. Required for normal water homeostasis and to prevent excessive water loss through the skin, probably via an indirect effect on the expression levels of other proteins, since CLDN1 itself seems to be dispensable for water barrier formation in keratinocyte tight junctions (PubMed:23407391).
(Microbial infection) Acts as a receptor for hepatitis C virus in hepatocytes (PubMed:17325668). Acts as a receptor for dengue virus (PubMed:24074594).
组织特异性

Strongly expressed in liver and kidney. Expressed in heart, brain, spleen, lung and testis.
疾病相关

Ichthyosis-sclerosing cholangitis neonatal syndrome
序列相似性

Belongs to the claudin family.
细胞定位

Cell junction, tight junction. Cell membrane.
Target information above from: UniProt accession O95832 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
数据库链接
- Entrez Gene: 9076 Human
- Entrez Gene: 12737 Mouse
- Entrez Gene: 65129 Rat
- Omim: 603718 Human
- SwissProt: O95832 Human
- SwissProt: O88551 Mouse
- SwissProt: P56745 Rat
- Unigene: 439060 Human
- Unigene: 289441 Mouse
- Unigene: 24293 Rat
see all
别名
- Claudin-1 antibody
- Claudin1 antibody
- CLD 1 antibody
- CLD1 antibody
- CLD1_HUMAN antibody
- CLDN 1 antibody
- Cldn1 antibody
- ILVASC antibody
- SEMP 1 antibody
- SEMP1 antibody
- Senescence associated epithelial membrane protein 1 antibody
- Senescence associated epithelial membrane protein antibody
- Senescence-associated epithelial membrane protein antibody
see all

Western blot - Anti-Claudin 1 antibody [RM1186] (ab317709)

All lanes : Anti-Claudin 1 antibody [RM1186] (ab317709) at 1/1000 dilution

Lane 1 : A549 (human lung carcinoma epithelial cell) whole cell lysate
Lane 2 : HepG2 (human hepatocellar carcinoma epithelial cell) whole cell lysate
Lane 3 : T-47D (human ductal breast epithelial tumor epithelial cell) whole cell lysate
Lane 4 : MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate
Lane 5 : HT-29 (human colorectal adenocarcinoma epithelial cell) whole cell lysate
Lane 6 : Wide-type A431 (human epidermoid carcinoma epithelial cell) whole cell lysate
Lane 7 : CLDN1 knockout A431 whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 22 kDa
Observed band size: 18 kDa why is the actual band size different from the predicted?

Exposure time: 15 seconds

Blocking and diluting buffer and concentration: 5% NFDM/TBST.

Low expression: MCF7 (PMID:30658316).

In Western blot, ab317709 was shown to bind specifically to Claudin 1. Target of interest was observed at 18 kDa, in wild-type A431 cell lysates (lane 6) with no signal observed at this size in CLDN1 knockout cell line (lane 7) (lane 7, knockout cell line ab261889 / knockout cell lysate ab261698).

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.
Western blot - Anti-Claudin 1 antibody [RM1186] (ab317709)

All lanes : Anti-Claudin 1 antibody [RM1186] (ab317709) at 1/1000 dilution

Lane 1 : Mouse colon tissue lysate
Lane 2 : Mouse liver tissue lysate
Lane 3 : Mouse brain tissue lysate
Lane 4 : Rat colon tissue lysate
Lane 5 : Rat liver tissue lysate
Lane 6 : Rat brain tissue lysate
Lane 7 : NMuLi (mouse liver epithelial) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 22 kDa
Observed band size: 18 kDa why is the actual band size different from the predicted?

Exposure time: 180 seconds

Blocking and diluting buffer and concentration: 5% NFDM/TBST.

Low expression: brain (PMID: 9647647).

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin 1 antibody [RM1186] (ab317709)

Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Claudin 1 with ab317709 at 1/100 (4.97 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on human colon. The section was incubated with ab317709 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin 1 antibody [RM1186] (ab317709)

Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling Claudin 1 with ab317709 at 1/100 (4.97 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on human liver. The section was incubated with ab317709 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin 1 antibody [RM1186] (ab317709)

Immunohistochemical analysis of paraffin-embedded Human gastric adenocarcinoma tissue labeling Claudin 1 with ab317709 at 1/100 (4.97 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on human gastric adenocarcinoma. The section was incubated with ab317709 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin 1 antibody [RM1186] (ab317709)

Immunohistochemical analysis of paraffin-embedded Mouse colon tissue labeling Claudin 1 with ab317709 at 1/100 (4.97 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on mouse colon. The section was incubated with ab317709 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin 1 antibody [RM1186] (ab317709)

Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling Claudin 1 with ab317709 at 1/100 (4.97 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on mouse liver. The section was incubated with ab317709 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin 1 antibody [RM1186] (ab317709)

Immunohistochemical analysis of paraffin-embedded Mouse breast carcinoma tissue labeling Claudin 1 with ab317709 at 1/100 (4.97 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on mouse breast carcinoma. The section was incubated with ab317709 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin 1 antibody [RM1186] (ab317709)

Immunohistochemical analysis of paraffin-embedded Rat colon tissue labeling Claudin 1 with ab317709 at 1/100 (4.97 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on rat colon. The section was incubated with ab317709 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin 1 antibody [RM1186] (ab317709)

Immunohistochemical analysis of paraffin-embedded Human cardiac muscle tissue labeling Claudin 1 with ab317709 at 1/100 (4.97 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Negative control: no staining on human cardiac muscle. The section was incubated with ab317709 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin 1 antibody [RM1186] (ab317709)

Immunohistochemical analysis of paraffin-embedded Mouse cardiac muscle tissue labeling Claudin 1 with ab317709 at 1/100 (4.97 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Negative control: no staining on mouse cardiac muscle. The section was incubated with ab317709 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin 1 antibody [RM1186] (ab317709)

Immunohistochemical analysis of paraffin-embedded Rat cardiac muscle tissue labeling Claudin 1 with ab317709 at 1/100 (4.97 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Negative control: no staining on rat cardiac muscle. The section was incubated with ab317709 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin 1 antibody [RM1186] (ab317709)

Immunohistochemical analysis of paraffin-embedded (A) Wild-type A431 (human epidermal carcinoma cells) cell pellet (B) CLDN1 knockout A431 cell pellet tissue labeling Claudin 1 with ab317709 at 1/500 (0.994 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on (A) Wild-type A431 cell pellet, no staining on (B) CLDN1 knockout A431 cell pellet. The section was incubated with ab317709 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunocytochemistry/ Immunofluorescence - Anti-Claudin 1 antibody [RM1186] (ab317709)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Tween-20 permeabilized CLDN1 KO A431(CLDN1 knockout human epidermoid carcinoma epithelial cell) cells labelling Claudin 1 with ab317709 at 1/100 (4.97 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green).
Confocal image showing membranous and cytoplasmic staining in wildtype A431 cells (shown in green) and showing no staining in CLDN1 knockout A431 cells. The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue).
Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).
ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.
Flow Cytometry (Intracellular) - Anti-Claudin 1 antibody [RM1186] (ab317709)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized CLDN1 KO A431(human epidermoid carcinoma epithelial cell, Left) / Parental A431(Right) cells labelling Claudin 1 with ab317709 at 1/500 dilution (0.1 ug)/Red (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.
Immunoprecipitation - Anti-Claudin 1 antibody [RM1186] (ab317709)

Claudin 1 was immunoprecipitated from 0.35 mg Mouse colon tissue lysate with ab317709 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab317709 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: Mouse colon tissue lysate
Lane 2: ab317709 IP in Mouse colon tissue lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab317709 in mouse colon tissue lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 180 seconds.
Immunoprecipitation - Anti-Claudin 1 antibody [RM1186] (ab317709)

Claudin 1 was immunoprecipitated from 0.35 mg NMuLi (mouse liver epithelial) whole cell lysate with ab317709 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab317709 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: NMuLi (mouse liver epithelial) whole cell lysate
Lane 2: ab317709 IP in NMuLi (mouse liver epithelial) whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab317709 in NMuLi whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 180 seconds.
Immunoprecipitation - Anti-Claudin 1 antibody [RM1186] (ab317709)

Claudin 1 was immunoprecipitated from 0.35 mg A549 (human lung carcinoma epithelial cell) whole cell lysate with ab317709 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab317709 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: A549 (human lung carcinoma epithelial cell) whole cell lysate
Lane 2: ab317709 IP in A549 (human lung carcinoma epithelial cell) whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab317709 in A549 whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 32 seconds.
Immunoprecipitation - Anti-Claudin 1 antibody [RM1186] (ab317709)

Claudin 1 was immunoprecipitated from 0.35 mg Rat liver tissue lysate with ab317709 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab317709 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: Rat liver tissue lysate
Lane 2: ab317709 IP in Rat liver tissue lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab317709 in rat liver tissue lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 seconds.
Anti-Claudin 1 antibody [RM1186] (ab317709)

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

SDS download

Country/Region

Language
Datasheet download

Download

发表研究结果有使用 ab317709？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab317709 尚未被引用在任何文献中。

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Switch on our new BETA site

Now available

In the coming months

Key features and details

Related conjugates and formulations

概述

产品名称

描述

宿主

特异性

经测试应用

种属反应性

免疫原

阳性对照

常规说明

性能

形式

存放说明

存储溶液

纯度

克隆

克隆编号

同种型

研究领域

相关产品

Alternative Versions

Compatible Secondaries

Isotype control

KO cell lines

KO cell lysates

Related Products

应用

The Abpromise guarantee

靶标

功能

组织特异性

疾病相关

序列相似性

细胞定位

数据库链接

别名

图片

实验方案

数据表及文件

SDS download

Datasheet download

文献 (0)

客户评价及客户问答