重组Anti-Claudin 1抗体[EPR9306] (ab180158)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9306] to Claudin 1
- Suitable for: WB
- Knockout validated
- Reacts with: Mouse, Human
Related conjugates and formulations
概述
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产品名称
Anti-Claudin 1抗体[EPR9306]
参阅全部 Claudin 1 一抗 -
描述
兔单克隆抗体[EPR9306] to Claudin 1 -
宿主
Rabbit -
特异性
Claudin-1 expression is minimal in normal colon tissues and is increased in tumors (PMID: 17308096,PMID: 15965503).
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经测试应用
适用于: WBmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Rat -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: A431, Human skin and fetal liver lysates, Mouse kidney and liver tissue lysates.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR9306 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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KO cell lines
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KO cell lysates
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Positive Controls
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab180158于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB | (1) |
1/2000 - 1/10000. Detects a band of approximately 19 kDa (predicted molecular weight: 23 kDa).
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说明 |
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WB
1/2000 - 1/10000. Detects a band of approximately 19 kDa (predicted molecular weight: 23 kDa). |
靶标
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功能
Claudins function as major constituents of the tight junction complexes that regulate the permeability of epithelia. While some claudin family members play essential roles in the formation of impermeable barriers, others mediate the permeability to ions and small molecules. Often, several claudin family members are coexpressed and interact with each other, and this determines the overall permeability. CLDN1 is required to prevent the paracellular diffusion of small molecules through tight junctions in the epidermis and is required for the normal barrier function of the skin. Required for normal water homeostasis and to prevent excessive water loss through the skin, probably via an indirect effect on the expression levels of other proteins, since CLDN1 itself seems to be dispensable for water barrier formation in keratinocyte tight junctions (PubMed:23407391).
(Microbial infection) Acts as a receptor for hepatitis C virus in hepatocytes (PubMed:17325668). Acts as a receptor for dengue virus (PubMed:24074594). -
组织特异性
Strongly expressed in liver and kidney. Expressed in heart, brain, spleen, lung and testis. -
疾病相关
Ichthyosis-sclerosing cholangitis neonatal syndrome -
序列相似性
Belongs to the claudin family. -
细胞定位
Cell junction, tight junction. Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 9076 Human
- Entrez Gene: 12737 Mouse
- Entrez Gene: 65129 Rat
- Omim: 603718 Human
- SwissProt: O95832 Human
- SwissProt: O88551 Mouse
- SwissProt: P56745 Rat
- Unigene: 439060 Human
see all -
别名
- Claudin-1 antibody
- Claudin1 antibody
- CLD 1 antibody
see all
图片
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All lanes : Anti-Claudin 1 antibody [EPR9306] (ab180158) at 1/10000 dilution
Lane 1 : Wild-type A-431 (Human epidermoid carcinoma cell line) whole cell lysate
Lane 2 : CLDN1 knockout A-431 (Human epidermoid carcinoma cell line) whole cell lysate
Lane 3 : A549 (Human lung carcinoma cell line) whole cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 23 kDaLanes 1 - 3: Merged signal (red and green). Green - ab180158 observed at 18 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab180158 was shown to specifically react with in wild-type A431 WT cells as signal was lost in A431 CLDN1 knockout cells. Wild-type and A431 CLDN1 knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3pc Milk. Ab180158 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/10000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
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Anti-Claudin 1 antibody [EPR9306] (ab180158) at 1/2000 dilution + Human fetal liver lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab136636) at 1/500 dilution
Predicted band size: 23 kDa -
All lanes : Anti-Claudin 1 antibody [EPR9306] (ab180158) at 1/10000 dilution
Lane 1 : A431 cell lysate
Lane 2 : Human skin lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 23 kDa -
All lanes : Anti-Claudin 1 antibody [EPR9306] (ab180158) at 1/1000 dilution
Lane 1 : Mouse liver tissue lysate
Lane 2 : Mouse kidney tissue lysate
Lane 3 : Mouse colon tissue lysate
Lane 4 : Mouse small intestine tissue lysate
Lane 5 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 23 kDa
Observed band size: 18 kDa why is the actual band size different from the predicted?
Exposure time: 60 secondsBlocking/Diluting buffer and concentration: 5% NFDM/TBST.
Claudin-1 expression is minimal in normal colon tissues and is increased in tumors (PMID: 17308096, PMID: 15965503).
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (33)
ab180158 被引用在 33 文献中.
- Shon WJ et al. Gut taste receptor type 1 member 3 is an intrinsic regulator of Western diet-induced intestinal inflammation. BMC Med 21:165 (2023). PubMed: 37118698
- He J et al. Intestinal changes in permeability, tight junction and mucin synthesis in a mouse model of Alzheimer's disease. Int J Mol Med 52:N/A (2023). PubMed: 37830152
- Lin H et al. Polymeric immunoglobulin receptor deficiency exacerbates autoimmune hepatitis by inducing intestinal dysbiosis and barrier dysfunction. Cell Death Dis 14:68 (2023). PubMed: 36709322
- He Y et al. Inhibitory effects of the nanoscale lysate derived from xenogenic dental pulp stem cells in lung cancer models. J Nanobiotechnology 21:488 (2023). PubMed: 38105218
- Yang J et al. Formononetin alleviates acute pancreatitis by reducing oxidative stress and modulating intestinal barrier. Chin Med 18:78 (2023). PubMed: 37370098