重组Anti-Claudin 1抗体[EPR25359-48] - BSA and Azide free (ab307693)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR25359-48] to Claudin 1 - BSA and Azide free
- Suitable for: WB, IHC-P, ICC/IF, IP
- Knockout validated
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-Claudin 1抗体[EPR25359-48] - BSA and Azide free
参阅全部 Claudin 1 一抗 -
描述
兔单克隆抗体[EPR25359-48] to Claudin 1 - BSA and Azide free -
宿主
Rabbit -
特异性
ICC/IF: Unsuitable for mouse and rat samples.
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经测试应用
适用于: WB, IHC-P, ICC/IF, IPmore details
不适用于: Flow Cyt or IHC-Fr -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: A431, Mouse liver, kidney and colon, Rat liver and kidney lysates. IHC-P: Human colon, gastric adenocarcinoma, Mouse colon, breast cancer, Rat colon and parental A431 cell pellet. ICC/IF: CLDN1 KO A431 cells. IP: Mouse liver cell.
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常规说明
ab307693 is the carrier-free version of ab307692
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
pH: 7.20
Constituent: 100% PBS -
无载体
是 -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR25359-48 -
研究领域
相关产品
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Alternative Versions
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Related Products
- VeriBlot for IP Detection Reagent (HRP) (ab131366)
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081)
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
- Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602)
- Human CLDN1 knockout A-431 cell line (ab261889)
- Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab307693于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 22 kDa.
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IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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ICC/IF |
Use at an assay dependent concentration.
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IP |
Use at an assay dependent concentration.
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说明 |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 22 kDa. |
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ICC/IF
Use at an assay dependent concentration. |
IP
Use at an assay dependent concentration. |
靶标
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功能
Claudins function as major constituents of the tight junction complexes that regulate the permeability of epithelia. While some claudin family members play essential roles in the formation of impermeable barriers, others mediate the permeability to ions and small molecules. Often, several claudin family members are coexpressed and interact with each other, and this determines the overall permeability. CLDN1 is required to prevent the paracellular diffusion of small molecules through tight junctions in the epidermis and is required for the normal barrier function of the skin. Required for normal water homeostasis and to prevent excessive water loss through the skin, probably via an indirect effect on the expression levels of other proteins, since CLDN1 itself seems to be dispensable for water barrier formation in keratinocyte tight junctions (PubMed:23407391).
(Microbial infection) Acts as a receptor for hepatitis C virus in hepatocytes (PubMed:17325668). Acts as a receptor for dengue virus (PubMed:24074594). -
组织特异性
Strongly expressed in liver and kidney. Expressed in heart, brain, spleen, lung and testis. -
疾病相关
Ichthyosis-sclerosing cholangitis neonatal syndrome -
序列相似性
Belongs to the claudin family. -
细胞定位
Cell junction, tight junction. Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 9076 Human
- Entrez Gene: 12737 Mouse
- Entrez Gene: 65129 Rat
- Omim: 603718 Human
- SwissProt: O95832 Human
- SwissProt: O88551 Mouse
- SwissProt: P56745 Rat
- Unigene: 439060 Human
see all -
别名
- Claudin-1 antibody
- Claudin1 antibody
- CLD 1 antibody
see all
图片
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All lanes : Anti-Claudin 1 antibody [EPR25359-48] (ab307692) at 1/1000 dilution
Lane 1 : Mouse liver tissue lysate 20 µg
Lane 2 : Mouse kidney tissue lysate 20 µg
Lane 3 : Mouse colon tissue lysate 20 µg
Lane 4 : Rat liver tissue lysate 20 µg
Lane 5 : Rat kidney tissue lysate 20 µg
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution
Predicted band size: 22 kDa
Observed band size: 19 kDa why is the actual band size different from the predicted?This data was developed using ab307692, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
This blot was developed using a high sensitivity ECL substrate.
Anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution.
Exposure time: 180 seconds
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All lanes : Anti-Claudin 1 antibody [EPR25359-48] (ab307692) at 1/1000 dilution
Lane 1 : A431 (human epidermoid carcinoma epithelial cell) whole cell lysate 20 µg
Lane 2 : CLDN1 knockout A431 whole cell lysate 20 µg
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution
Predicted band size: 22 kDa
Observed band size: 19 kDa why is the actual band size different from the predicted?This data was developed using ab307692, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
In Western blot, ab307692 was shown to bind specifically to Claudin 1. A band was observed at 19 kDa in A431 cell lysates whereas no signal was observed at this size in the CLDN1 knockout cell line (ab261889). Anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution.
Exposure time: 15 seconds
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This data was developed using ab307692, the same antibody clone in a different buffer formulation.
Claudin 1 was immunoprecipitated from 0.35 mg Mouse liver tissue lysate 10 μg with ab307692 at 1/30 dilution (2 μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab307692 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: Mouse liver tissue lysate 10 μg
Lane 2: ab307692 IP in Mouse liver tissue lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab307692 in mouse liver whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 24 seconds
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Immunohistochemical analysis of paraffin-embedded (A) Parental A431 (h tissue labeling Claudin 1 with ab307692 at 1/500 (1.028 µg/ml) followed by a ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Positive staining on parental A431 (human epidermal carcinoma cells) cell pellet, negative staining on CLDN1 knockout A431 cell pellet (ab261889). The section was incubated with ab307692 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond® Polymer Refine Detection).Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
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Immunohistochemical analysis of paraffin-embedded Rat colon tissue labeling Claudin 1 with ab307692 at 1/100 (5.14 µg/ml) followed by a ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Membranous staining on rat colon. The section was incubated with ab307692 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond® Polymer Refine Detection).Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
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Immunohistochemical analysis of paraffin-embedded Mouse breast cancer tissue labeling Claudin 1 with ab307692 at 1/100 (5.14 µg/ml) followed by a ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Membranous staining on mouse breast cancer. The section was incubated with ab307692 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond® Polymer Refine Detection).Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
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Immunohistochemical analysis of paraffin-embedded Mouse colon tissue labeling Claudin 1 with ab307692 at 1/100 (5.14 µg/ml) followed by a ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Membranous staining on mouse colon (PMID: 24626855). The section was incubated with ab307692 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond® Polymer Refine Detection).Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
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Immunohistochemical analysis of paraffin-embedded Human gastric adenoc tissue labeling Claudin 1 with ab307692 at 1/100 (5.14 µg/ml) followed by a ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Membranous staining on human gastric adenocarcinoma (PMID: 24535143). The section was incubated with ab307692 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond® Polymer Refine Detection).Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
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Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Claudin 1 with ab307692 at 1/100 (5.14 µg/ml) followed by a ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Membranous staining on human colon (PMID: 25038829). The section was incubated with ab307692 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond® Polymer Refine Detection).Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
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This data was developed using ab307692, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized CLDN1 KO A431(CLDN1 knockout human epidermoid carcinoma epithelial cell) cells labelling Claudin 1 with ab307692 at 1/50 (10.28 µg/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 µg/ml dilution (Green). Confocal image showing membranous and cytoplasmic staining in wildtype A431 cells and showing no staining in CLDN1 knockout A431 cells. Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5µg/ml dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 µg/ml dilution.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
Certificate of Compliance
文献 (0)
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