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AB15098

Anti-Claudin 1 抗体

Anti-Claudin 1 antibody

4

(21 Reviews)

|

(231 Publications)

Anti-Claudin 1 antibody (ab15098) is a rabbit polyclonal antibody detecting Claudin 1 in Western Blot, IHC-P, ICC/IF. Suitable for Human.

- Over 230 publications
- Trusted since 2004

查看别名

CLD1, SEMP1, UNQ481/PRO944, CLDN1, Claudin-1, Senescence-associated epithelial membrane protein

3 Images
Western blot - Anti-Claudin 1 antibody (AB15098)
  • WB

Unknown

Western blot - Anti-Claudin 1 antibody (AB15098)

All lanes:

Western blot - Anti-Claudin 1 antibody (ab15098) at 1/25 dilution

All lanes:

HeLa cell lysate

Predicted band size: 22 kDa

Observed band size: 19 kDa

false

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin 1 antibody (AB15098)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin 1 antibody (AB15098)

ab15098 (1µg/ml) staining Claudin 1 in human skin using an automated system (DAKO Autostainer Plus). Using this protocol there is strong staining of the cell membrane.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.

Immunocytochemistry/ Immunofluorescence - Anti-Claudin 1 antibody (AB15098)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Claudin 1 antibody (AB15098)

ICC/IF image of ab15098 stained Hek293 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab15098, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

No

反应种属

Human

应用

WB, IHC-P, ICC/IF

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

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您是否考虑以下替代产品？

AB211737

Anti-Claudin 1 antibody [EPRR18871]

5
7 Reviews
查看产品
为什么推荐这个？

为向您提供最高品质的产品，我们在不断升级产品范围。我们选择了这个替代产品供您参考，因为它提供了更好的性能，更容易和更方便的使用，或交期更快。

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产品详情

What is this antibody validated in?
Anti-Claudin 1 antibody (ab15098) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human samples.

What is the molecular weight of Claudin 1?
Anti-Claudin 1 (ab15098) specifically detects a band for Claudin 1 (UniProt: O95832) at a molecular weight of 23kDa.

Trusted by the scientific community
Anti-Claudin 1 (ab15098) was first used in a scientific publication in 2004 and has been cited over 230 times in peer-reviewed journals.

Reviewed by scientists
Anti-Claudin 1 (ab15098) has over 20 independent reviews from customers.
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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Immunogen
存储溶液
pH: 7.6 Preservative: 0.1% Sodium azide Constituents: PBS, 1% BSA
运输条件
Blue Ice
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Claudin 1 also known as CLDN1 is a protein that plays a fundamental role in the formation of tight junctions in the epithelial cells. This claudin protein has a molecular weight of approximately 22 kDa. You can find it expressed in various tissues including the skin liver kidney and lungs. Claudin 1 belongs to the claudin family which consists of transmembrane proteins involved in sealing the space between adjacent cells therefore ensuring tissue integrity.
Biological function summary

This claudin protein contributes to establishing and maintaining the barrier function that controls paracellular transport which is the movement of substances between cells. Claudin 1 participates as a critical component of the tight junction complex and it interacts with other proteins such as occludin and zonula occludens. These interactions contribute to the regulation and maintenance of cell polarity and signaling.

Pathways

Claudin 1 is involved in the epithelial cell signaling pathways that influence cellular proliferation and differentiation. One significant pathway is the Wnt signaling pathway where claudin 1 cooperates with other claudins and proteins like Β-catenin to regulate gene transcription. Additionally it is implicated in the MAPK pathway which involves signaling cascades that affect cell growth division and response to external stress.

Claudin 1 shows a strong association with cancers particularly hepatocellular carcinoma and melanoma. In these conditions changes in claudin 1 expression levels can affect tumor progression and metastasis. It also associates with other proteins like E-cadherin impacting cell adhesion and epithelial-mesenchymal transition in cancerous cells. Moreover abnormalities in claudin 1 expression relate to skin disorders such as ichthyosis which is marked by a dysfunction in the skin barrier.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Claudins function as major constituents of the tight junction complexes that regulate the permeability of epithelia. While some claudin family members play essential roles in the formation of impermeable barriers, others mediate the permeability to ions and small molecules. Often, several claudin family members are coexpressed and interact with each other, and this determines the overall permeability. CLDN1 is required to prevent the paracellular diffusion of small molecules through tight junctions in the epidermis and is required for the normal barrier function of the skin. Required for normal water homeostasis and to prevent excessive water loss through the skin, probably via an indirect effect on the expression levels of other proteins, since CLDN1 itself seems to be dispensable for water barrier formation in keratinocyte tight junctions (PubMed : 23407391).. (Microbial infection) Acts as a co-receptor for hepatitis C virus (HCV) in hepatocytes (PubMed : 17325668, PubMed : 20375010, PubMed : 24038151). Associates with CD81 and the CLDN1-CD81 receptor complex is essential for HCV entry into host cell (PubMed : 20375010). Acts as a receptor for dengue virus (PubMed : 24074594).
See full target information CLDN1
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文献 (231)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 24: PubMed38003573

2023

Lipids Reduce Cytokine-Induced Pro-Inflammatory Signalling and Barrier Dysfunction in Human Keratinocyte Models.

Applications

Unspecified application

Species

Unspecified reactive species

Jamie M L Kok,Georgina C Dowd,Jaydee D Cabral,Lyn M Wise

Animals : an open access journal from MDPI 13: PubMed38003115

2023

Dietary Supplementation with Lysozyme-Cinnamaldehyde Conjugates Enhances Feed Conversion Efficiency by Improving Intestinal Health and Modulating the Gut Microbiota in Weaned Piglets Infected with Enterotoxigenic .

Applications

Unspecified application

Species

Unspecified reactive species

Zhezhe Tian,Jiaming Chen,Tongbin Lin,Junhua Zhu,Haoyang Gan,Fang Chen,Shihai Zhang,Wutai Guan

Frontiers in molecular biosciences 10:1253195 PubMed37711388

2023

Protective effect of carbon dots derived from scrambled against ulcerative colitis in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Yanfang Mou,Xue Bai,Huagen Ma,Tingjie Li,Yafang Zhao,Tong Wu,Yue Zhang,Huihua Qu,Hui Kong,Xueqian Wang,Yan Zhao

Heliyon 9:e19110 PubMed37809985

2023

LncSUMO1P3 exacerbates malignant behaviors of esophageal squamous cell carcinoma cells via miR-486-5p/PHF8/CD151.

Applications

Unspecified application

Species

Unspecified reactive species

Tingyou Zhang,Guojun Yue,Xin Tian,Ying Xu,Zhongwen Li

Journal of gastroenterology and hepatology 38:2006-2017 PubMed37608570

2023

Regulation of epithelial cell differentiation by the Ubiquitous expressed transcript isoform 1 in ulcerative colitis.

Applications

Unspecified application

Species

Unspecified reactive species

Wu Peng,Chengcheng Zeng,Jing Xu,Hailan Zhao,Qingqing Zhu,Haoming Xu,Huiting Chen,Hongli Huang,Yongjian Zhou,Chong Zhao

Cells 12: PubMed37566066

2023

Adhesion GPCR Gpr126 (Adgrg6) Expression Profiling in Zebrafish, Mouse, and Human Kidney.

Applications

Unspecified application

Species

Unspecified reactive species

Salvador Cazorla-Vázquez,Peter Kösters,Simone Bertz,Frederick Pfister,Christoph Daniel,Mark Dedden,Sebastian Zundler,Tilman Jobst-Schwan,Kerstin Amann,Felix B Engel

NPJ breast cancer 9:35 PubMed37160903

2023

Reversion of breast epithelial polarity alterations caused by obesity.

Applications

Unspecified application

Species

Unspecified reactive species

Julia Holmes,Mohamed Gaber,Mónica Z Jenks,Adam Wilson,Tucker Loy,Cassandra Lepetit,Mara Z Vitolins,Brittney-Shea Herbert,Katherine L Cook,Pierre-Alexandre Vidi

Cellular and molecular gastroenterology and hepatology 16:83-105 PubMed37011811

2023

DR3 Regulates Intestinal Epithelial Homeostasis and Regeneration After Intestinal Barrier Injury.

Applications

Unspecified application

Species

Unspecified reactive species

Yosuke Shimodaira,Shyam K More,Hussein Hamade,Anna Y Blackwood,Jay P Abraham,Lisa S Thomas,Jordan H Miller,Dalton T Stamps,Sofi L Castanon,Noam Jacob,Connie W Y Ha,Suzanne Devkota,David Q Shih,Stephan R Targan,Kathrin S Michelsen

Annals of translational medicine 11:34 PubMed36819593

2023

MicroRNA-155-5p inhibition alleviates irritable bowel syndrome by increasing claudin-1 and ZO-1 expression.

Applications

Unspecified application

Species

Unspecified reactive species

Jian-Guo Guo,Yue-Feng Rao,Jun Jiang,Xin Li,Sheng-Mei Zhu

Scientific reports 13:2520 PubMed36781906

2023

Tanshinone IIA-loaded nanoparticles and neural stem cell combination therapy improves gut homeostasis and recovery in a pig ischemic stroke model.

Applications

Unspecified application

Species

Unspecified reactive species

Julie H Jeon,Erin E Kaiser,Elizabeth S Waters,Xueyuan Yang,Jeferson M Lourenco,Madison M Fagan,Kelly M Scheulin,Sydney E Sneed,Soo K Shin,Holly A Kinder,Anil Kumar,Simon R Platt,Jeongyoun Ahn,Kylee J Duberstein,Michael J Rothrock,Todd R Callaway,Jin Xie,Franklin D West,Hea Jin Park
View all publications
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