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Anti-Clathrin light chain抗体(ab77542)

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Western blot - Anti-Clathrin light chain antibody (ab77542)

    Key features and details

    • Goat polyclonal to Clathrin light chain
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

    概述

    • 产品名称

      Anti-Clathrin light chain抗体
      参阅全部 Clathrin light chain 一抗

    • 描述

      山羊多克隆抗体to Clathrin light chain

    • 宿主

      Goat

    • 特异性

      Expected to recognize all reported isoforms of light chains a and b.

    • 经测试应用

      适用于: WBmore details

    • 种属反应性

      与反应: Human
      预测可用于: Mouse, Rat, Horse, Chicken, Cow, Dog, Pig, Xenopus laevis, Chimpanzee, Zebrafish, Macaque monkey

    • 免疫原

      Synthetic peptide:

      CDFNPKSSKQAKD

      from Human Clathrin light chain(NP_001824.1; NP_009027.1; NP_001070145.1; NP_001825.1; NP_009028.1)
      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

    • 阳性对照

      • Recombinant Human Clathrin light chain protein (ab126693) can be used as a positive control in WB. Human frontal cortex lysate

    • 常规说明

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    性能

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab77542于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    WB
    说明
    应用说明
    ELISA: Use at an assay dependent dilution. Antibody detection limit dilution: 1/32000.
    WB: Use at a concentration of 0.1 - 0.3 µg/ml. Predicted molecular weight: 23 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    靶标

    图片

    • Western blot - Anti-Clathrin light chain antibody (ab77542)
      Western blot - Anti-Clathrin light chain antibody (ab77542)
      Anti-Clathrin light chain antibody (ab77542) at 0.1 µg/ml + Human frontal cortex lysate in RIPA buffer at 35 µg

      Predicted band size: 23 kDa
      Observed band size: 23 kDa
      Additional bands at: 35 kDa (possible isoform)



      The observed molecular weight of ~35kDa corresponds to earlier findings in literature with different antibodies (Okamoto et al, Am J Physiol Cell Physiol. 2000 Sep;279(3):C833-51.; PMID: 10942733)

    文献 (1)

    发表研究结果有使用 ab77542？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab77542 被引用在 1 文献中.

    • Liu H  et al. Clathrin-mediated endocytosis in living host cells visualized through quantum dot labeling of infectious hematopoietic necrosis virus. J Virol 85:6252-62 (2011). WB . PubMed: 21525360

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