Anti-Clathrin light chain抗体(ab129326)
Key features and details
- Goat polyclonal to Clathrin light chain
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-Clathrin light chain抗体
参阅全部 Clathrin light chain 一抗 -
描述
山羊多克隆抗体to Clathrin light chain -
宿主
Goat -
特异性
ab129326 is expected to recognize all reported isoforms of light chains a and b. -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat, Horse, Chicken, Cow, Dog, Pig, Xenopus laevis, Zebrafish, Gorilla
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免疫原
Synthetic peptide:
CDFNPKSSKQAKD
, corresponding to internal sequence amino acids 218-230 of Human Clathrin light chain a (NP_009027.1). -
阳性对照
- Human Spleen tissue; Human Frontal Cortex lysate.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab129326于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 23, 35 kDa (predicted molecular weight: 23 kDa).
|
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| IHC-P |
Use a concentration of 2.5 - 3.75 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| 说明 |
|---|
|
WB
Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 23, 35 kDa (predicted molecular weight: 23 kDa). |
|
IHC-P
Use a concentration of 2.5 - 3.75 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
Clathrin is the major protein of the polyhedral coat of coated pits and vesicles. -
细胞定位
Cytoplasmic vesicle membrane. Membrane > coated pit. Cytoplasmic face of coated pits and vesicles. - Information by UniProt
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数据库链接
- Entrez Gene: 416226 Chicken
- Entrez Gene: 427284 Chicken
- Entrez Gene: 281078 Cow
- Entrez Gene: 281079 Cow
- Entrez Gene: 281698 Cow
- Entrez Gene: 474765 Dog
- Entrez Gene: 489104 Dog
- Entrez Gene: 100055375 Horse
see all -
别名
- Clathrin light chain A antibody
- Clathrin light chain B antibody
- Clathrin light chain LCA antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Clathrin light chain antibody (ab129326)ab129326, at 2.5 µg/ml, staining Clathrin light chain in formalin-fixed, paraffin-embedded Human spleen tissue by Immunohistochemistry.
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Western blot - Anti-Clathrin light chain antibody (ab129326)Anti-Clathrin light chain antibody (ab129326) at 0.1 µg/ml + Human Frontal Cortex lysate in RIPA buffer at 35 µg
Developed using the ECL technique.
Predicted band size: 23 kDa
Primary incubation was 1 hour.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab129326 尚未被引用在任何文献中。