Anti-Clathrin heavy chain抗体(ab21679)
Key features and details
- Rabbit polyclonal to Clathrin heavy chain
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-Clathrin heavy chain抗体
参阅全部 Clathrin heavy chain 一抗 -
描述
兔多克隆抗体to Clathrin heavy chain -
宿主
Rabbit -
特异性
Replenishment batches of our polyclonal antibody, ab21679 are tested in WB. Previous batches were additionally validated in ICC/IF. This application is still expected to work and is covered by our Abpromise guarantee. You may also be interested in our alternative recombinant antibody, ab172958.
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经测试应用
适用于: WB, ICC/IFmore details -
种属反应性
与反应: Mouse, Rat, Human
预测可用于: Chicken, Hamster
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免疫原
Synthetic peptide conjugated to KLH derived from within residues 1650 to the C-terminus of Human Clathrin heavy chain.
参阅Abcam的专有抗源政策 (Peptide available as ab23440.) -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab21679于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (13) |
Use a concentration of 1 µg/ml. Detects a band of approximately 180 kDa (predicted molecular weight: 180 kDa).
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| ICC/IF | (19) |
Use a concentration of 1 µg/ml.
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| 说明 |
|---|
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 180 kDa (predicted molecular weight: 180 kDa). |
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ICC/IF
Use a concentration of 1 µg/ml. |
靶标
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功能
Clathrin is the major protein of the polyhedral coat of coated pits and vesicles. Two different adapter protein complexes link the clathrin lattice either to the plasma membrane or to the trans-Golgi network. -
序列相似性
Belongs to the clathrin heavy chain family. -
细胞定位
Cytoplasmic vesicle membrane. Membrane > coated pit. Melanosome. Cytoplasmic face of coated pits and vesicles. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
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数据库链接
- Entrez Gene: 1213 Human
- Entrez Gene: 67300 Mouse
- Entrez Gene: 54241 Rat
- Omim: 118955 Human
- SwissProt: P53675 Human
- SwissProt: Q00610 Human
- SwissProt: Q68FD5 Mouse
- SwissProt: P11442 Rat
see all -
别名
- CHC17 antibody
- Clathrin heavy chain antibody
- Clathrin heavy chain 1 antibody
see all
图片
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Western blot - Anti-Clathrin heavy chain antibody (ab21679)All lanes : Anti-Clathrin heavy chain antibody (ab21679) at 1 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : A431 whole cell lysate
Lane 3 : NIH 3T3 whole cell lysate
Lane 4 : PC12 whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/50000 dilution
Predicted band size: 180 kDa
Observed band size: 180 kDaBlocking buffer: 2% BSA
Gel type: TA
Exposure Time: 1 minute
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Immunocytochemistry/ Immunofluorescence - Anti-Clathrin heavy chain antibody (ab21679)ab21679 staining Clathrin heavy chain in HeLa cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab21679 at 1µg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.
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Western blot - Anti-Clathrin heavy chain antibody (ab21679)This image is courtesy of an anonymous AbreviewAnti-Clathrin heavy chain antibody (ab21679) at 1/1000 dilution + HUVEC cell lysate at 20 µg
Secondary
HRP conjugated goat anti-rabbit IgG at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 180 kDa
Exposure time: 5 seconds
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Immunocytochemistry/ Immunofluorescence - Anti-Clathrin heavy chain antibody (ab21679)This image is courtesy of an anonymous AbreviewImmunocytochemical analysis of mouse bEnd.3 labeling Clathrin heavy chain with ab21679 at 1/300 dilution using secondary anti-rabbit-alexa fluor 488 at 1/500 dilution.
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Immunocytochemistry/ Immunofluorescence - Anti-Clathrin heavy chain antibody (ab21679)This image is a courtesy of Anonymous Abreviewab21679 staining Clathrin heavy chain in mouse embryonic fibroblasts by Immunocytochemistry/ Immunofluorescence. Cells were fixed with paraformaldehyde and permeabilized with -20°C ethanol. Samples were incubated with primary antibody at 1/500 dilution (in 0.1% Saponin/1% BSA/PBS) for 1 hour. An Alexa Fluor® 546-conjugated goat polyclonal to rabbit IgG (H+L) was used as secondary antibody at 1/500 dilution. Green color in image show positive staining with Alexa Fluor® 546 conjugated secondary.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (145)
ab21679 被引用在 145 文献中.
- Chan CWF et al. High-throughput screening of genetic and cellular drivers of syncytium formation induced by the spike protein of SARS-CoV-2. Nat Biomed Eng 8:291-309 (2024). PubMed: 37996617
- Kell M et al. Immunoprecipitation and Western Blot Analysis of AP-1 Clathrin-Coated Vesicles. Methods Mol Biol 2557:619-633 (2023). PubMed: 36512241
- Kvalvaag A et al. Clathrin mediates both internalization and vesicular release of triggered T cell receptor at the immunological synapse. Proc Natl Acad Sci U S A 120:e2211368120 (2023). PubMed: 36730202
- Mund M et al. Clathrin coats partially preassemble and subsequently bend during endocytosis. J Cell Biol 222:N/A (2023). PubMed: 36734980
- Lores S et al. Effectiveness of a novel gene nanotherapy based on putrescine for cancer treatment. Biomater Sci 11:4210-4225 (2023). PubMed: 36790445