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Anti-Claspin抗体(ab3720)

Reviews (5)Q&A (1)References (14)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claspin antibody (ab3720)

    Key features and details

    • Rabbit polyclonal to Claspin
    • Suitable for: IHC-P
    • Reacts with: Human
    • Isotype: IgG

    概述

    • 产品名称

      Anti-Claspin抗体
      参阅全部 Claspin 一抗

    • 描述

      兔多克隆抗体to Claspin

    • 宿主

      Rabbit

    • 经测试应用

      适用于: IHC-Pmore details

    • 种属反应性

      与反应: Human
      预测可用于: Chimpanzee, Gorilla, Orangutan

    • 免疫原

      Synthetic peptide (Human) - which represented a portion of human Claspin encoded in part by exons 11, 12 and 13.

    • 常规说明

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    性能

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab3720于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    IHC-P
    1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
    说明
    IHC-P
    1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    靶标

    • 功能

      Required for checkpoint mediated cell cycle arrest in response to inhibition of DNA replication or to DNA damage induced by both ionizing and UV irradiation. Adapter protein which binds to BRCA1 and the checkpoint kinase CHEK1 and facilitates the ATR-dependent phosphorylation of both proteins. Can also bind specifically to branched DNA structures and may associate with S-phase chromatin following formation of the pre-replication complex (pre-RC). This may indicate a role for this protein as a sensor which monitors the integrity of DNA replication forks.

    • 序列相似性

      Belongs to the claspin family.

    • 结构域

      The C-terminus of the protein contains 3 potential CHEK1-binding motifs (CKB motifs). Potential phosphorylation sites within CKB motif 1 and CKB motif 2 are required for interaction with CHEK1.

    • 翻译后修饰

      Phosphorylated. Undergoes ATR-dependent phosphorylation by CHEK1 during activation of DNA replication or damage checkpoints. Phosphorylation by CSNK1G1/CK1 promotes CHEK1 binding.
      Ubiquitinated by the anaphase promoting complex/cyclosome (APC/C) during G1 phase, leading to its degradation by the proteasome. Ubiquitination is mediated via its interaction with FZR1/CDH1. Following DNA damage, it is deubiquitinated by USP28 in G2 phase, preventing its degradation.

    • 细胞定位

      Nucleus.
    • Information by UniProt

    • 数据库链接

      • 别名

        • Claspin antibody
        • claspin homolog (Xenopus laevis) antibody
        • Claspin homolog antibody
        • CLSPN antibody
        • CLSPN protein antibody
        • CLSPN_HUMAN antibody
        • hClaspin antibody
        • Hu Claspin antibody
        see all

      图片

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claspin antibody (ab3720)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claspin antibody (ab3720)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung carcinoma tissue labelling Claspin with ab3720 at 1/1000 (1µg/ml). Detection: DAB.

      文献 (14)

      发表研究结果有使用 ab3720？请让我们知道，以便我们可以引用本数据表中的参考文章。

      ab3720 被引用在 14 文献中.

      • Kobayashi G  et al. Claspin overexpression is associated with high-grade histology and poor prognosis in renal cell carcinoma. Cancer Sci 111:1020-1027 (2020). PubMed: 31912588
      • Rai R  et al. The Replisome Mediates A-NHEJ Repair of Telomeres Lacking POT1-TPP1 Independently of MRN Function. Cell Rep 29:3708-3725.e5 (2019). PubMed: 31825846
      • Liang X  et al. Anti-TGF-ß Antibody, 1D11, Ameliorates Glomerular Fibrosis in Mouse Models after the Onset of Proteinuria. PLoS One 11:e0155534 (2016). IHC . PubMed: 27187580
      • Ho H  et al. RhoJ regulates melanoma chemoresistance by suppressing pathways that sense DNA damage. Cancer Res 72:5516-28 (2012). PubMed: 22971344
      • Yoshida K  et al. CDC6 interaction with ATR regulates activation of a replication checkpoint in higher eukaryotic cells. J Cell Sci 123:225-35 (2010). WB ; Human . PubMed: 20048340
      View all Publications for this product

      客户评价及客户问答

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      1-6 of 6 Abreviews or Q&A

      Western blot abreview for Anti-Claspin antibody

       Good
      Abreviews
      abreview image
      Application
      Western blot
      Loading amount
      40 µg
      Gel Running Conditions
      Reduced Denaturing (4-12% Bis-Tris)
      Sample
      Human Cell lysate - nuclear (HeLa nuclear cell lysate)
      Specification
      HeLa nuclear cell lysate
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Abcam user community

      Verified customer

      提交于 Aug 08 2013

      Western blot abreview for Anti-Claspin antibody

       Average
      Abreviews
      Application
      Western blot
      Sample
      Human Cell lysate - whole cell (HEK 293)
      Loading amount
      20 µg
      Specification
      HEK 293
      Gel Running Conditions
      Non-reduced Denaturing (7.5)
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Abcam user community

      Verified customer

      提交于 Nov 07 2011

      Immunoprecipitation abreview for Anti-Claspin antibody

       Good
      Abreviews
      abreview image
      Application
      Immunoprecipitation
      Sample
      Human Cell lysate - whole cell (HT29 tumour cell line)
      Total protein in input
      500 µg
      Specification
      HT29 tumour cell line
      Treatment
      4Gy Ionising Radiation
      Immuno-precipitation step
      Protein A/G
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Abcam user community

      Verified customer

      提交于 Aug 13 2009

      Western blot abreview for Anti-Claspin antibody

       Excellent
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Human Cell lysate - whole cell (ht29 tumour cell line)
      Loading amount
      50 µg
      Specification
      ht29 tumour cell line
      Treatment
      4Gy Ionising Radiation
      Gel Running Conditions
      Reduced Denaturing
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Mr. Paul Eve

      Verified customer

      提交于 Aug 07 2009

      Immunocytochemistry/ Immunofluorescence abreview for Anti-Claspin antibody

       Average
      Abreviews
      abreview image
      Application
      Immunocytochemistry/ Immunofluorescence
      Sample
      Human Cell (HeLa)
      Specification
      HeLa
      Fixative
      Paraformaldehyde
      Permeabilization
      Yes - 0.5% TritonX100 in PBS
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      DR. Kirk Mcmanus

      Verified customer

      提交于 May 28 2009

      Question

      I recently bought this ab, which, as written in the datasheet, detects a band of approximately 175 kDa. I completed a western blot analysis in Whole human cell lysate (MDA-MB-231 breast cancer cells) treated with increasing doses of the proteasomal inhibitor MG-132 and detected various bands; ˜270, ˜240, ˜175, ˜160, ˜125, ˜100 and ˜70 KDa. Previously, I was using an anti-Claspin antibody (from another company) that was detecting a band at ˜220kDa in these cell line. Since my strongest bands are the 270 and 240 kDa, I was wondering whether this is the actual claspin band(s) and not the 175/160 kDa?

      Also, has anybody else reported detecting two bands? It seems that I detect a lot of double bands.

      ·

      Read More
      Answer

      The predicted MW of the protein is 151 kDa for isoform 1. Two other isoforms exist, isoform 2 (144 kDa) and isoform 3 (150 kDa) (see http://www.uniprot.org/uniprot/Q9HAW4), but I am not sure under which conditions you would be able to detect either one and in which samples they might be present. The literature might be able to help in this regard.

      As for your WB results, the band should be around 175 - 180 kDa that's where the lab and other customers detected the band (see Abreviews). I cannot find an explanation for the 2 bands around 250 kDa as well as the other bands you see around 130, 100 and 70 kDa. I believe these must be non-specific bands.

      Read More

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