Anti-Citrate synthetase抗体[2H8BB6] (ab128564)
Key features and details
- Mouse monoclonal [2H8BB6] to Citrate synthetase
- Suitable for: IP, ICC/IF
- Reacts with: Human
- Isotype: IgG2b
概述
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产品名称
Anti-Citrate synthetase抗体[2H8BB6]
参阅全部 Citrate synthetase 一抗 -
描述
小鼠单克隆抗体[2H8BB6] to Citrate synthetase -
宿主
Mouse -
经测试应用
适用于: IP, ICC/IFmore details -
种属反应性
与反应: Human
不与反应: Mouse, Rat -
免疫原
Tissue, cells or virus. This information is considered to be commercially sensitive.
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阳性对照
- Human Liver Mitochondria;
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常规说明
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Product was previously marketed under the MitoSciences sub-brand.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
存储溶液
pH: 7.5
Preservative: 0.02% Sodium azide
Constituent: 99% HEPES buffered saline -
Concentration information loading...
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纯度
Ammonium Sulphate Precipitation -
纯化说明
Purity is near homogeneity as judged by SDS-PAGE. The antibody was produced in vitro using hybridomas grown in serum-free medium, and then concentrated by ammonium sulfate precipitation. -
克隆
单克隆 -
克隆编号
2H8BB6 -
同种型
IgG2b -
轻链类型
kappa -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab128564于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IP |
Use at an assay dependent concentration.
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ICC/IF |
Use a concentration of 5 µg/ml.
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说明 |
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IP
Use at an assay dependent concentration. |
ICC/IF
Use a concentration of 5 µg/ml. |
靶标
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通路
Carbohydrate metabolism; tricarboxylic acid cycle; isocitrate from oxaloacetate: step 1/2. -
序列相似性
Belongs to the citrate synthase family. -
细胞定位
Mitochondrion matrix. - Information by UniProt
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数据库链接
- Entrez Gene: 1431 Human
- Omim: 118950 Human
- SwissProt: O75390 Human
- Unigene: 430606 Human
- Unigene: 728168 Human
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别名
- CISY_HUMAN antibody
- Citrate synthase antibody
- Citrate synthase, mitochondrial antibody
see all
图片
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Immunoprecipitation using ab128564
Lane 1: Ladder
Lane 2: Human Liver Mitochondria -
Immunofluorescent staining of Citrate synthetase in Hela cells (human) using ab128564.
The cells were paraformaldehyde fixed (4%, 20 min) and Triton X-100 permeabilized (0.1%, 15min). The cells were then incubated with the antibody (ab128564, 5µg/ml) for 2h at room temperature or overnight at 4°C. The secondary antibody was (red) Alexa Fluor® 594 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. 1% BSA was used as the blocking agent for all blocking steps. The target protein locates to the mitochondrial matrix.
实验方案
数据表及文件
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Datasheet download
文献 (3)
ab128564 被引用在 3 文献中.
- Villani R et al. Eradication of HCV by direct antiviral agents restores mitochondrial function and energy homeostasis in peripheral blood mononuclear cells. FASEB J 36:e22650 (2022). PubMed: 36394523
- Askeland G et al. Increased nuclear DNA damage precedes mitochondrial dysfunction in peripheral blood mononuclear cells from Huntington's disease patients. Sci Rep 8:9817 (2018). WB ; Human . PubMed: 29959348
- Rhein VF et al. Human METTL12 is a mitochondrial methyltransferase that modifies citrate synthase. FEBS Lett 591:1641-1652 (2017). PubMed: 28391595