AB128564

Anti-Citrate synthetase抗体[2H8BB6]

Name: Citrate synthetase抗体[2H8BB6] (ab128564)| Abcam中文官网
Brand: Abcam Limited
SKU: AB128564
Price: 4503 CNY
Availability: InStock

Anti-Citrate synthetase antibody [2H8BB6]

Be the first to review this product! Submit a review

(3 Publications)

Mouse Monoclonal Citrate synthetase antibody. Suitable for IP, ICC/IF and reacts with Human samples. Cited in 3 publications.

查看别名

Citrate (Si)-synthase, CS

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-Citrate synthetase antibody [2H8BB6] (AB128564)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Citrate synthetase antibody [2H8BB6] (AB128564)

Immunofluorescent staining of Citrate synthetase in Hela cells (human) using ab128564.

The cells were paraformaldehyde fixed (4%, 20 min) and Triton X-100 permeabilized (0.1%, 15min). The cells were then incubated with the antibody (ab128564, 5µg/ml) for 2h at room temperature or overnight at 4°C. The secondary antibody was (red) Alexa Fluor® 594 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. 1% BSA was used as the blocking agent for all blocking steps. The target protein locates to the mitochondrial matrix.

Unknown

Immunoprecipitation - Anti-Citrate synthetase antibody [2H8BB6] (AB128564)

Immunoprecipitation using ab128564

Lane 1 : Ladder
Lane 2 : Human Liver Mitochondria

All lanes:

Immunoprecipitation - Anti-Citrate synthetase antibody [2H8BB6] (ab128564)

Predicted band size: 51 kDa

false

关键信息

宿主种属

Mouse

克隆

Monoclonal

克隆号

2H8BB6

亚型

IgG2b

轻链类型

kappa

不含载体蛋白

反应种属

Human

应用

ICC/IF, IP

applications

style

left-column

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "5 µg/mL", "ICCIF-species-notes": "" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "5 µg/mL", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "5 µg/mL", "ICCIF-species-notes": "" } } }

style

left-column

产品详情

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

style

left-column

性能和储存信息

形式

Liquid

纯化工艺

Precipitation Ammonium Sulphate

纯化说明

Purity is near homogeneity as judged by SDS-PAGE. The antibody was produced in vitro using hybridomas grown in serum-free medium, and then concentrated by ammonium sulfate precipitation.

存储溶液

pH: 7.5 Preservative: 0.02% Sodium azide Constituents: 99% HEPES buffered saline

运输条件

Blue Ice

储存信息

Do Not Freeze

style

left-column

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Citrate synthetase also known as citrate synthase is an important enzyme in the tricarboxylic acid cycle. It catalyzes the condensation of acetyl-CoA and oxaloacetate to form citrate and CoA. The enzyme weighs around 49 kilodaltons. It is expressed prominently in the mitochondria of eukaryotic cells where it initiates the Krebs cycle by providing citrate to be further processed. Citrate synthetase is an important point of control within this metabolic cycle.

Biological function summary

Citrate synthetase plays a central role in energy production by converting oxaloacetate and acetyl-CoA into citrate. This enzyme is not known to be part of any larger complex but associates with downstream enzymes such as aconitase in the metabolic pathway. Its activity is essential for cellular respiration impacting the overall metabolic rate of the organism. The concentration of citrate produced serves as a checkpoint both for the continuation of the Krebs cycle and for feedback inhibition of glycolysis.

Pathways

Citrate synthetase is integral to the Krebs cycle and the related oxidative phosphorylation pathway. These pathways are essential for efficient ATP production. Citrate synthetase works closely with enzymes like isocitrate dehydrogenase and alpha-ketoglutarate dehydrogenase within the Krebs cycle. Through these interactions citrate synthetase ensures the proper flow of carbon through the cycle impacting ATP yield and cellular energy homeostasis.

Citrate synthetase's function can influence metabolic diseases like diabetes and mitochondrial disorders. Alterations in its activity may contribute to the dysregulation of glucose metabolism seen in diabetes affecting enzymes like glucose transporter 4 (GLUT4). In mitochondrial disorders changes in its normal activity can cause energy production deficiencies influencing proteins such as cytochrome c which is critical in the electron transport chain. Understanding these interactions can help develop therapeutic strategies targeting metabolic pathways.

style

left-column

style

left-column

产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

靶点信息

Key enzyme of the Krebs tricarboxylic acid cycle which catalyzes the synthesis of citrate from acetyl coenzyme A and oxaloacetate.

See full target information Citrate synthase, mitochondrial

style

left-column

文献 (3)

Recent publications for all applications. Explore the full list and refine your search

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 36:e22650 PubMed36394523

2022

Eradication of HCV by direct antiviral agents restores mitochondrial function and energy homeostasis in peripheral blood mononuclear cells.

Applications

Unspecified application

Species

Unspecified reactive species

Rosanna Villani,Moris Sangineto,Paola Pontrelli,Francesco Bellanti,Vidyasagar N Bukke,Archana Moola,Loreto Gesualdo,Gianluigi Vendemiale,Giuseppe Grandaliano,Giovanni Stallone,Gaetano Serviddio

Scientific reports 8:9817 PubMed29959348

2018

Increased nuclear DNA damage precedes mitochondrial dysfunction in peripheral blood mononuclear cells from Huntington's disease patients.

Applications

Species

Human

Georgina Askeland,Zaneta Dosoudilova,Marie Rodinova,Jiri Klempir,Irena Liskova,Anna Kuśnierczyk,Magnar Bjørås,Gaute Nesse,Arne Klungland,Hana Hansikova,Lars Eide

FEBS letters 591:1641-1652 PubMed28391595

2017

Human METTL12 is a mitochondrial methyltransferase that modifies citrate synthase.

Applications

Unspecified application

Species

Unspecified reactive species

Virginie F Rhein,Joe Carroll,Shujing Ding,Ian M Fearnley,John E Walker

View all publications

style

left-column

style

left-column

style

right-column

Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。

详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Citrate synthetase抗体[2H8BB6]

Anti-Citrate synthetase antibody [2H8BB6]

Immunocytochemistry/ Immunofluorescence - Anti-Citrate synthetase antibody [2H8BB6] (AB128564)

Immunoprecipitation - Anti-Citrate synthetase antibody [2H8BB6] (AB128564)

关键信息

宿主种属

克隆

克隆号

亚型

轻链类型

不含载体蛋白

反应种属

应用