AB108345

重组Anti-CITED2抗体[EPR3416(2)]

Anti-CITED2 antibody [EPR3416(2)]

RabMAb
Recombinant
了解详情

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(10 Publications)

Rabbit Recombinant Monoclonal CITED2 antibody. Suitable for WB and reacts with Mouse, Human samples. Cited in 10 publications.

查看别名

MRG1, CITED2, Cbp/p300-interacting transactivator 2, MSG-related protein 1, P35srj, MRG-1

5 Images

Lab

Western blot - Anti-CITED2 antibody [EPR3416(2)] (AB108345)

In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/1000000 dilution.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-CITED2 antibody [EPR3416(2)] (ab108345) at 1/1000 dilution

Lane 1:

HeLa (human cervix adenocarcinoma epithelial cell) + si-Scramble whole cell lysate at 20 µg

Lane 2:

HeLa + si-CITED2 whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 30 kDa,36 kDa

false

Exposure time: 80s

Lab

Western blot - Anti-CITED2 antibody [EPR3416(2)] (AB108345)

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-CITED2 antibody [EPR3416(2)] (ab108345) at 20 µg

All lanes:

Saos-2 whole cell lysate at 20 µg

Secondary

All lanes:

Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 28 kDa,53 kDa,94 kDa

Observed band size: 100 kDa,28 kDa,50 kDa

false

Lab

Western blot - Anti-CITED2 antibody [EPR3416(2)] (AB108345)

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-CITED2 antibody [EPR3416(2)] (ab108345) at 1/1000 dilution

All lanes:

U937 whole cell lysate at 20 µg

Secondary

All lanes:

Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 28 kDa

Observed band size: 28 kDa

false

Unknown

Western blot - Anti-CITED2 antibody [EPR3416(2)] (AB108345)

All lanes:

Western blot - Anti-CITED2 antibody [EPR3416(2)] (ab108345) at 1/1000 dilution

Lane 1:

Saos-2 cell lysate at 10 µg

Lane 2:

U937 cell lysate at 10 µg

Lane 3:

HeLa cell lysate at 10 µg

Predicted band size: 28 kDa

false

Lab

Western blot - Anti-CITED2 antibody [EPR3416(2)] (AB108345)

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-CITED2 antibody [EPR3416(2)] (ab108345) at 1/2000 dilution

All lanes:

NIH/3T3 whole cell lysate at 10 µg

Secondary

All lanes:

Peroxidase-conjugated goat anti-rabbit IgG, (H+L) at 1/1000 dilution

Predicted band size: 103 kDa,21 kDa,28 kDa,42 kDa,68 kDa

Observed band size: 120 kDa,28 kDa,42 kDa,74 kDa

false

不同偶联物与剂型 (1)

Carrier free

Anti-CITED2 antibody [EPR3416(2)] - BSA and Azide free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR3416(2)

亚型

IgG

不含载体蛋白

反应种属

Mouse, Human

应用

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "" } } }

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产品详情

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Species reactivity
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

运输条件

Conditional Ambient

分装信息

Upon delivery aliquot

储存信息

Stable for 12 months at -20°C

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Transcriptional coactivator of the p300/CBP-mediated transcription complex. Acts as a bridge, linking TFAP2 transcription factors and the p300/CBP transcriptional coactivator complex in order to stimulate TFAP2-mediated transcriptional activation. Positively regulates TGF-beta signaling through its association with the SMAD/p300/CBP-mediated transcriptional coactivator complex. Stimulates the peroxisome proliferator-activated receptors PPARA transcriptional activity. Enhances estrogen-dependent transactivation mediated by estrogen receptors. Also acts as a transcriptional corepressor; interferes with the binding of the transcription factors HIF1A or STAT2 and the p300/CBP transcriptional coactivator complex. Participates in sex determination and early gonad development by stimulating transcription activation of SRY. Plays a role in controlling left-right patterning during embryogenesis; potentiates transcriptional activation of NODAL-mediated gene transcription in the left lateral plate mesoderm (LPM). Plays an essential role in differentiation of the adrenal cortex from the adrenogonadal primordium (AGP); stimulates WT1-mediated transcription activation thereby up-regulating the nuclear hormone receptor NR5A1 promoter activity. Associates with chromatin to the PITX2 P1 promoter region.

See full target information CITED2

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文献 (10)

Recent publications for all applications. Explore the full list and refine your search

Nature genetics 57:2468-2481 PubMed41044247

2025

Targeting histone H2B acetylated enhanceosomes via p300/CBP degradation in prostate cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Luo,Zhixiang Chen,Yuanyuan Qiao,Jean Ching-Yi Tien,Eleanor Young,Rahul Mannan,Somnath Mahapatra,Rupam Bhattacharyya,Lanbo Xiao,Tongchen He,Sanjana Eyunni,Yuping Zhang,Yang Zheng,Fengyun Su,Xuhong Cao,Rui Wang,Yunhui Cheng,Rithvik Seri,James George,Miriam Shahine,Stephanie J Miner,Matthew G Rees,Melissa M Ronan,Jennifer A Roth,Ulka Vaishampayan,Mi Wang,Shaomeng Wang,Abhijit Parolia,Arul M Chinnaiyan

International heart journal 65:1087-1094 PubMed39537169

2024

CITED2 Attenuates Ischemia Reperfusion-Induced Pyroptosis and Injury in Cardiomyocyte.

Applications

Unspecified application

Species

Unspecified reactive species

Long Qian,Juan Zhao,Mengtao Fan,Jie Wang,Zhuqing Ji

The Journal of biological chemistry 294:8617-8629 PubMed30967472

2019

The pro-death role of Cited2 in stroke is regulated by E2F1/4 transcription factors.

Applications

Unspecified application

Species

Unspecified reactive species

Tianwen Huang,Yasmilde Rodríguez González,Dianbo Qu,En Huang,Farzaneh Safarpour,Eugene Wang,Alvin Joselin,Doo Soon Im,Steve M Callaghan,Wassamon Boonying,Lisa Julian,Sally L Dunwoodie,Ruth S Slack,David S Park

International journal of oncology 54:2019-2029 PubMed30942439

2019

MAT2B promotes proliferation and inhibits apoptosis in osteosarcoma by targeting epidermal growth factor receptor and proliferating cell nuclear antigen.

Applications

Unspecified application

Species

Unspecified reactive species

Yuan Yuan,Yonggang Wang,Zimei Liu,Yong Sun,Yang Yao,Wenxi Yu,Zan Shen

The Journal of biological chemistry 293:11109-11118 PubMed29794136

2018

The transcription factor Vezf1 represses the expression of the antiangiogenic factor Cited2 in endothelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Lama AlAbdi,Ming He,Qianyi Yang,Allison B Norvil,Humaira Gowher

Oncotarget 9:6707-6717 PubMed29467921

2018

Endoplasmic reticulum stress stimulates the release of extracellular vesicles carrying danger-associated molecular pattern (DAMP) molecules.

Applications

Unspecified application

Species

Unspecified reactive species

Gavin P Collett,Christopher W Redman,Ian L Sargent,Manu Vatish

Nature communications 7:13147 PubMed27874008

2016

The GCN5-CITED2-PKA signalling module controls hepatic glucose metabolism through a cAMP-induced substrate switch.

Applications

Unspecified application

Species

Mouse

Mashito Sakai,Tomoko Tujimura-Hayakawa,Takashi Yagi,Hiroyuki Yano,Masaru Mitsushima,Hiroyuki Unoki-Kubota,Yasushi Kaburagi,Hiroshi Inoue,Yoshiaki Kido,Masato Kasuga,Michihiro Matsumoto

The Journal of neuroscience : the official journal of the Society for Neuroscience 36:6403-19 PubMed27307230

2016

Cited2 Regulates Neocortical Layer II/III Generation and Somatosensory Callosal Projection Neuron Development and Connectivity.

Applications

Unspecified application

Species

Unspecified reactive species

Ryann M Fame,Jessica L MacDonald,Sally L Dunwoodie,Emi Takahashi,Jeffrey D Macklis

Reproduction (Cambridge, England) 151:509-16 PubMed26917451

2016

CITED2 modulation of trophoblast cell differentiation: insights from global transcriptome analysis.

Applications

Unspecified application

Species

Unspecified reactive species

Kazuhiko Imakawa,Pramod Dhakal,Kaiyu Kubota,Kazuya Kusama,Damayanti Chakraborty,M A Karim Rumi,Michael J Soares

PloS one 10:e0137079 PubMed26325290

2015

High-Throughput siRNA Screening to Reveal GATA-2 Upstream Transcriptional Mechanisms in Hematopoietic Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yo Saito,Tohru Fujiwara,Keiichi Ohashi,Yoko Okitsu,Noriko Fukuhara,Yasushi Onishi,Kenichi Ishizawa,Hideo Harigae

View all publications

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Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。

详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

重组Anti-CITED2抗体[EPR3416(2)]