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Anti-CISH/CIS抗体(ab88383)

Submit a review Q&A (2)References (2)
Western blot - Anti-CISH/CIS antibody (ab88383)
  • Western blot - Anti-CISH/CIS antibody (ab88383)
  • Immunocytochemistry/ Immunofluorescence - Anti-CISH/CIS antibody (ab88383)

Key features and details

  • Mouse polyclonal to CISH/CIS
  • Suitable for: WB, ICC/IF
  • Reacts with: Human
  • Isotype: IgG

概述

  • 产品名称

    Anti-CISH/CIS抗体
    参阅全部 CISH/CIS 一抗

  • 描述

    小鼠多克隆抗体to CISH/CIS

  • 宿主

    Mouse

  • 经测试应用

    适用于: WB, ICC/IFmore details

  • 种属反应性

    与反应: Human

  • 免疫原

    Recombinant full length protein within Human CISH/CIS. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
    Database link: NP_659508.1

  • 阳性对照

    • Human spleen lysate. CISH/CIS transfected 293T cell lysate. HeLa cells.

  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab88383于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 29 kDa.
ICC/IF
Use a concentration of 10 µg/ml.
说明
WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 29 kDa.
ICC/IF
Use a concentration of 10 µg/ml.

靶标

  • 功能

    SOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. CIS is involved in the negative regulation of cytokines that signal through the JAK-STAT5 pathway such as erythropoietin, prolactin and interleukin 3 (IL3) receptor. Inhibits STAT5 trans-activation by suppressing its tyrosine phosphorylation. May be a substrate-recognition component of a SCF-like ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins.

  • 组织特异性

    Expressed in various epithelial tissues. Abundantly expressed in liver and kidney, and to a lesser extent in lung. The tissue distribution of isoforms 1 and 1B is distinct.

  • 通路

    Protein modification; protein ubiquitination.

  • 序列相似性

    Contains 1 SH2 domain.
    Contains 1 SOCS box domain.

  • 翻译后修饰

    Association with EPOR may target the protein for proteolysis by the ubiquitin-dependent proteasome pathway. CIS is mainly monubiquitinated (37 kDa form) but may also exist in a polyubiquitinated form (45 kDa).
  • Information by UniProt

  • 数据库链接

    • 别名

      • BACTS2 antibody
      • cis 1 antibody
      • CIS antibody
      • CIS-1 antibody
      • CIS1 antibody
      • Cish antibody
      • CISH_HUMAN antibody
      • Cytokine inducible inhibitor of signaling type 1B antibody
      • Cytokine inducible SH2 containing protein antibody
      • Cytokine-inducible SH2-containing protein antibody
      • G18 antibody
      • Protein G18 antibody
      • SOCS antibody
      • Suppressor of cytokine signaling antibody
      see all

    图片

    • Western blot - Anti-CISH/CIS antibody (ab88383)
      Western blot - Anti-CISH/CIS antibody (ab88383)
      Anti-CISH/CIS antibody (ab88383) at 1 µg/ml + human spleen tissue lysate at 50 µg

      Predicted band size: 29 kDa
      Observed band size: 37 kDa why is the actual band size different from the predicted?

    • Western blot - Anti-CISH/CIS antibody (ab88383)
      Western blot - Anti-CISH/CIS antibody (ab88383)
      All lanes : Anti-CISH/CIS antibody (ab88383) at 1 µg/ml

      Lane 1 : CISH/CIS transfected 293T cell lysate
      Lane 2 : Non-transfected 293T cell lysate

      Lysates/proteins at 25 µg per lane.

      Predicted band size: 29 kDa
      Observed band size: 35 kDa why is the actual band size different from the predicted?

    • Immunocytochemistry/ Immunofluorescence - Anti-CISH/CIS antibody (ab88383)
      Immunocytochemistry/ Immunofluorescence - Anti-CISH/CIS antibody (ab88383)

      Immunofluorescence analysis of CISH/CIS in HeLa cells using ab88383 at 10µg/ml.

    文献 (2)

    发表研究结果有使用 ab88383？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab88383 被引用在 2 文献中.

    • Jin J  et al. CISH impairs lysosomal function in activated T cells resulting in mitochondrial DNA release and inflammaging. Nat Aging 3:600-616 (2023). PubMed: 37118554
    • Clasen BF  et al. Gene expression in skeletal muscle after an acute intravenous GH bolus in human subjects: identification of a mechanism regulating ANGPTL4. J Lipid Res 54:1988-97 (2013). PubMed: 23606725

    客户评价及客户问答

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    1-2 of 2 Abreviews or Q&A

    Question

    WB with human CT8 cells
    Ab worked before
    this lot GR61646-4 : no band
    transfer OK, other proteins detected
    20 and 50 ug protein loaded
    Ab: 1ug/ml
    block: 5% block solution
    2nd: works well

    Read More
    Answer

    Thank you for contacting us.

    I am sorry to hear that the antibody is not working as previously.

    As we discussed over the phone, I have arranged for a free of charge replacement with a new lot number (GR89220-1) which you will receive with our next shipment.

    I apologize about any inconvenience caused.

    Please let me know if the new vials works as expected. I wish you good luck with your research!

    Read More

    Question

    Our lab has been working w/ your CISH and SOCS1-7 antibodies for the past
    year, and I was wondering if in that time abcam has changed a catalog
    number or discontinued any of its CISH antibodies?
    The reason I ask is because we are currently performing Western Blots w/
    ab88383 and the current product sheet says the expected band size is
    37KDa; however, when working with this antibody (same catalog number) last
    year the product sheet said the expected band size was 19KDa. Can you
    please shed some light on this confusion?

    Read More
    Answer

    Thank you for contacting us.

    I checked, but we did not unpublish any CISH antibody in the last couple years.
    Also, I did not see any changes made to the MW. Our internal notes do not indicate that.

    The predicted MWfor human CISH is 30 kda (http://www.uniprot.org/uniprot/Q9NSE2). CIS is mainly monubiquitinated (37 kDa form) but may also exist in a poly-ubiquitinated form (45 kDa). This is in agreement with our findings in the WB image on the datasheet.

    I am sorry about any confusion, but hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

    Read More

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