AB246510

重组Anti-CIRP抗体[EPR23628-36]

Name: CIRP抗体[EPR23628-36] (ab246510)| Abcam中文官网
Brand: Abcam Limited
SKU: AB246510
Price: 1758 CNY
Availability: InStock
Rating: 5 (2 reviews)

Anti-CIRP antibody [EPR23628-36]

RabMAb
Recombinant
了解详情

(2 Reviews)

(3 Publications)

Rabbit Recombinant Monoclonal CIRP antibody. Suitable for IP, WB and reacts with Mouse, Rat samples. Cited in 3 publications.

查看别名

Cirp, Cirbp, Cold-inducible RNA-binding protein, A18 hnRNP, Glycine-rich RNA-binding protein CIRP

4 Images

Unknown

Immunoprecipitation - Anti-CIRP antibody [EPR23628-36] (AB246510)

CIRBP was immunoprecipitated from 0.35 mg Mouse testis tissue lysate with ab246510 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab246510 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : Mouse testis tissue lysate 10ug

Lane 2 : ab246510 IP in Mouse testis tissue lysate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab246510 in Mouse testis tissue lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 47 seconds.

This blot was developed using a higher sensitivity ECL substrate.

All lanes:

Immunoprecipitation - Anti-CIRP antibody [EPR23628-36] (ab246510)

Predicted band size: 18 kDa

Observed band size: 18 kDa

false

Lab

Western blot - Anti-CIRP antibody [EPR23628-36] (AB246510)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This blot was developed using a higher sensitivity ECL substrate.

This antibody also recognized an undetermined band at around 50 kDa

Exposure time : 3 minutes.

All lanes:

Western blot - Anti-CIRP antibody [EPR23628-36] (ab246510) at 1/1000 dilution

Lane 1:

NIH/3T3 (mouse embryonic fibroblast) treated at 37°C for 48 hours, whole cell lysate at 20 µg

Lane 2:

NIH/3T3 treated at 32°C for 48 hours, whole cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 18 kDa

Observed band size: 18 kDa

false

Lab

Western blot - Anti-CIRP antibody [EPR23628-36] (AB246510)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Exposure time : 3 minutes.

All lanes:

Western blot - Anti-CIRP antibody [EPR23628-36] (ab246510) at 1/1000 dilution

Lane 1:

Mouse testis tissue lysate at 20 µg

Lane 2:

Rat testis tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 18 kDa

Observed band size: 18 kDa

false

Lab

Western blot - Anti-CIRP antibody [EPR23628-36] (AB246510)

Blocking and dilution buffer : 5% NFDM/TBST.

This blot was developed using a high sensitivity ECL substrate.

All lanes:

Western blot - Anti-CIRP antibody [EPR23628-36] (ab246510) at 1/1000 dilution

Lane 1:

Untreated NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate at 20 µg

Lane 2:

NIH/3T3 (Mouse embryonic fibroblast) treated with 32˚C for 48 h whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 18 kDa

Observed band size: 18 kDa

false

Exposure time: 180s

不同偶联物与剂型 (1)

Carrier free

Anti-CIRP antibody [EPR23628-36] - BSA and Azide free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR23628-36

亚型

IgG

不含载体蛋白

反应种属

Mouse, Rat

应用

WB, IP

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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产品详情

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Cold-inducible mRNA binding protein that plays a protective role in the genotoxic stress response by stabilizing transcripts of genes involved in cell survival. Promotes assembly of stress granules (SGs), when overexpressed. Seems to play an essential role in cold-induced suppression of cell proliferation. Acts as a translational repressor. Acts as a translational activator. Binds specifically to the 3'-untranslated regions (3'-UTRs) of stress-responsive transcripts RPA2 and TXN.

See full target information Cirbp

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文献 (3)

Recent publications for all applications. Explore the full list and refine your search

Communications biology 8:403 PubMed40065057

2025

CIRP contributes to multiple organ damage in acute pancreatitis by increasing endothelial permeability.

Applications

Unspecified application

Species

Unspecified reactive species

Wuming Liu,Derek H Wu,Tao Wang,Mengzhou Wang,Yujia Xu,Yifan Ren,Yi Lyu,Rongqian Wu

iScience 26:107043 PubMed37360693

2023

Targeting extracellular CIRP with an X-aptamer shows therapeutic potential in acute pancreatitis.

Applications

Unspecified application

Species

Unspecified reactive species

Wuming Liu,Jianbin Bi,Yifan Ren,Huan Chen,Jia Zhang,Tao Wang,Mengzhou Wang,Lin Zhang,Junzhou Zhao,Zheng Wu,Yi Lv,Bing Liu,Rongqian Wu

BioFactors (Oxford, England) 47:427-443 PubMed33638934

2021

Human antigen R (HuR) and Cold inducible RNA-binding protein (CIRP) influence intestinal mucosal barrier function in ulcerative colitis by competitive regulation on Claudin1.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Xu,Yuxi Tian,Ying Wang,Junwen Yang,Fujun Li,Xiaoping Wan,Miao Ouyang

View all publications

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Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。

详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

重组Anti-CIRP抗体[EPR23628-36]