Anti-CIRP抗体(ab106230)
Key features and details
- Goat polyclonal to CIRP
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
概述
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产品名称
Anti-CIRP抗体
参阅全部 CIRP 一抗 -
描述
山羊多克隆抗体to CIRP -
宿主
Goat -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Mouse, Rat, Human
预测可用于: Chicken, Cow, Dog, Cynomolgus monkey, Rainbow trout, Chinese hamster
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免疫原
Synthetic peptide corresponding to Human CIRP aa 161-172 (C terminal).
Sequence:C-RDSYDSYATHNE
Database link: NP_001271.1 -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, Tris buffered saline -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab106230于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use a concentration of 0.01 - 0.03 µg/ml. Detects a band of approximately 18 kDa (predicted molecular weight: 19 kDa).
1 hour primary incubation is recommended for this product. |
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| IHC-P |
Use a concentration of 3 - 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| 说明 |
|---|
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WB
Use a concentration of 0.01 - 0.03 µg/ml. Detects a band of approximately 18 kDa (predicted molecular weight: 19 kDa). 1 hour primary incubation is recommended for this product. |
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IHC-P
Use a concentration of 3 - 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
Cold-inducible mRNA binding protein that plays a protective role in the genotoxic stress response by stabilizing transcripts of genes involved in cell survival. Acts as a translational activator. Seems to play an essential role in cold-induced suppression of cell proliferation. Binds specifically to the 3'-untranslated regions (3'-UTRs) of stress-responsive transcripts RPA2 and TXN. Acts as a translational repressor (By similarity). Promotes assembly of stress granules (SGs), when overexpressed. -
组织特异性
Ubiquitous. -
序列相似性
Contains 1 RRM (RNA recognition motif) domain. -
结构域
Both the RRM domain and the arginine, glycine (RGG) rich domain are necessary for binding to the TXN 3'-untranslated region. Both the RRM domain and the arginine, glycine (RGG) rich domain (RGG repeats) are necessary for optimal recruitment into SGs upon cellular stress. The C-terminal domain containing RGG repeats is necessary for translational repression. -
翻译后修饰
Methylated on arginine residues. Methylation of the RGG motifs is a prerequisite for recruitment into SGs.
Phosphorylated by CK2, GSK3A and GSK3B. Phosphorylation by GSK3B increases RNA-binding activity to the TXN 3'-UTR transcript upon exposure to UV radiation. -
细胞定位
Nucleus > nucleoplasm. Cytoplasm. Translocates from the nucleus to the cytoplasm after exposure to UV radiation. Translocates from the nucleus to the cytoplasm into stress granules upon various cytoplasmic stresses, such as osmotic and heat shocks. Its recruitment into stress granules occurs in the absence of TIAR proteins. - Information by UniProt
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数据库链接
- Entrez Gene: 100689075 Chinese hamster
- Entrez Gene: 507120 Cow
- Entrez Gene: 476755 Dog
- Entrez Gene: 1153 Human
- Entrez Gene: 12696 Mouse
- Entrez Gene: 81825 Rat
- Omim: 602649 Human
- SwissProt: P60826 Chinese hamster
see all -
别名
- A18 hnRNP antibody
- A18HNRNP antibody
- cirbp antibody
see all
图片
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Western blot - Anti-CIRP antibody (ab106230)Anti-CIRP antibody (ab106230) at 0.01 µg/ml + mouse testis lysate in RIPA buffer at 35 µg
Developed using the ECL technique.
Predicted band size: 19 kDa
数据表及文件
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SDS download
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Datasheet download
文献 (7)
ab106230 被引用在 7 文献中.
- Liao Y et al. CIRP promotes the progression of non-small cell lung cancer through activation of Wnt/β-catenin signaling via CTNNB1. J Exp Clin Cancer Res 40:275 (2021). PubMed: 34465343
- Kübler M et al. The Absence of Extracellular Cold-Inducible RNA-Binding Protein (eCIRP) Promotes Pro-Angiogenic Microenvironmental Conditions and Angiogenesis in Muscle Tissue Ischemia. Int J Mol Sci 22:N/A (2021). PubMed: 34502391
- Zhou K et al. Cold-inducible RNA-binding protein contributes to intracerebral hemorrhage-induced brain injury via TLR4 signaling. Brain Behav 10:e01618 (2020). PubMed: 32285591
- Downie Ruiz Velasco A et al. Posttranscriptional Regulation of 14q32 MicroRNAs by the CIRBP and HADHB during Vascular Regeneration after Ischemia. Mol Ther Nucleic Acids 14:329-338 (2019). PubMed: 30665182
- Luo Y et al. Exogenous hydrogen sulfide ameliorates high glucose-induced myocardial injury & inflammation via the CIRP-MAPK signaling pathway in H9c2 cardiac cells. Life Sci N/A:N/A (2018). WB . PubMed: 29857073
- Rao M et al. The regulation of CIRBP by transforming growth factor beta during heat shock-induced testicular injury. Andrology N/A:N/A (2018). PubMed: 30461215
- Rao M et al. Isobaric tags for relative and absolute quantification-based proteomic analysis of testis biopsies in rhesus monkeys treated with transient scrotal hyperthermia. Oncotarget 8:85909-85925 (2017). PubMed: 29156766