重组Anti-Cip4抗体[EPR1965] (ab108313)
![Western blot - Anti-Cip4 antibody [EPR1965] (ab108313)](https://www.abcam.cn/ps/products/108/ab108313/Images/ab108313-410135-anti-cip4-antibody-epr1965-western-blot-human-knockout-lysate.jpg "Western blot - Anti-Cip4 antibody [EPR1965] (ab108313)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR1965] to Cip4
- Suitable for: WB, IHC-P
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-Cip4抗体[EPR1965]
参阅全部 Cip4 一抗 -
描述
兔单克隆抗体[EPR1965] to Cip4 -
宿主
Rabbit -
特异性
Recent WB re-tests performed by our lab suggest to use a higher dilution for mouse samples. The tested dilutions were 1:500 and 1:2000, were the signal detected was very strong. Human and rat samples worked fine in these dilutions. -
经测试应用
适用于: WB, IHC-Pmore details
不适用于: Flow Cyt,ICC/IF or IP -
种属反应性
与反应: Human
预测可用于: Mouse, Rat
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免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: HEK293T, JAR, HepG2, TF1, and HeLa cell lysates. IHC-P: Human stomach tissue.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
纯度
Tissue culture supernatant -
克隆
单克隆 -
克隆编号
EPR1965 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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KO cell lines
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KO cell lysates
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab108313于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 68 kDa.
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| IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
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| 说明 |
|---|
|
WB
1/1000 - 1/10000. Predicted molecular weight: 68 kDa. |
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IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. |
靶标
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功能
Required for translocation of GLUT4 to the plasma membrane in response to insulin signaling (By similarity). Required to coordinate membrane tubulation with reorganization of the actin cytoskeleton during endocytosis. Binds to lipids such as phosphatidylinositol 4,5-bisphosphate and phosphatidylserine and promotes membrane invagination and the formation of tubules. Also promotes CDC42-induced actin polymerization by recruiting WASL/N-WASP which in turn activates the Arp2/3 complex. Actin polymerization may promote the fission of membrane tubules to form endocytic vesicles. Required for the formation of podosomes, actin-rich adhesion structures specific to monocyte-derived cells. May be required for the lysosomal retention of FASLG/FASL. -
组织特异性
Expressed in brain, colon, heart, kidney, liver, lung, megakaryocyte, ovary, pancreas, peripheral blood lymphocytes, placenta, prostate, skeletal muscle, small intestine, spleen, testis, thymus and trachea. -
序列相似性
Belongs to the FNBP1 family.
Contains 1 FCH domain.
Contains 1 REM (Hr1) repeat.
Contains 1 SH3 domain. -
翻译后修饰
Tyrosine phosphorylated. Also phosphorylated by PKA. -
细胞定位
Cytoplasm > perinuclear region and Cytoplasm > cytoskeleton. Cytoplasm > cell cortex. Lysosome. Golgi apparatus. Cell membrane. Cell projection > phagocytic cup. Translocates to the plasma membrane in response to insulin stimulation, and this may require active RHOQ (By similarity). Localizes to cortical regions coincident with F-actin, to lysosomes and to sites of phagocytosis in macrophages. Also localizes to the Golgi, and this requires AKAP9. - Information by UniProt
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数据库链接
- Entrez Gene: 9322 Human
- Entrez Gene: 106628 Mouse
- Entrez Gene: 116717 Rat
- Omim: 604504 Human
- SwissProt: Q15642 Human
- SwissProt: Q8CJ53 Mouse
- SwissProt: P97531 Rat
- Unigene: 515094 Human
see all -
别名
- Cdc42 interacting protein 4 antibody
- Cdc42 interaction protein 4 long isoform antibody
- Cdc42-interacting protein 4 antibody
see all
图片
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Western blot - Anti-Cip4 antibody [EPR1965] (ab108313)All lanes : Anti-Cip4 antibody [EPR1965] (ab108313) at 1/1000 dilution
Lane 1 : Wild-type HEK293T cell lysate
Lane 2 : TRIP10 knockout HEK293T cell lysate
Lane 3 : JAR cell lysate
Lane 4 : HepG2 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 68 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?Lanes 1-4: Merged signal (red and green). Green - ab108313 observed at 75 kDa. Red - loading control ab8245 observed at 36 kDa.
ab108313 Anti-Cip4 antibody [EPR1965] was shown to specifically react with Cip4 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266428 (knockout cell lysate ab258251) was used. Wild-type and Cip4 knockout samples were subjected to SDS-PAGE. ab108313 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cip4 antibody [EPR1965] (ab108313)](https://www.abcam.cn/ps/products/108/ab108313/Images/ab108313-120867-anti-cip4-antibody-epr1965-immunohistochemistry.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cip4 antibody [EPR1965] (ab108313)ab108313 at 1/100 dilution staining Cip4 in paraffin embedded Human stomach tissue.
Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
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![Western blot - Anti-Cip4 antibody [EPR1965] (ab108313)](https://www.abcam.cn/ps/products/108/ab108313/Images/ab108313-120868-anti-cip4-antibody-epr1965-western-blot.jpg)
Western blot - Anti-Cip4 antibody [EPR1965] (ab108313)All lanes : Anti-Cip4 antibody [EPR1965] (ab108313) at 1/1000 dilution
Lane 1 : JAR cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : TF1 cell lysate
Lane 4 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 68 kDa -
![Anti-Cip4 antibody [EPR1965] (ab108313)](https://www.abcam.cn/ps/products/108/ab108313/Images/ab108313-5-benefits-of-recombinant-antibodies.png)
Anti-Cip4 antibody [EPR1965] (ab108313)
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab108313 被引用在 1 文献中.
- Meng DF et al. CDC42-interacting protein 4 promotes metastasis of nasopharyngeal carcinoma by mediating invadopodia formation and activating EGFR signaling. J Exp Clin Cancer Res 36:21 (2017). PubMed: 28129778