Anti-CIP2A抗体[2A1-9A4] (ab128179)
Key features and details
- Mouse monoclonal [2A1-9A4] to CIP2A
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG1
概述
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产品名称
Anti-CIP2A抗体[2A1-9A4]
参阅全部 CIP2A 一抗 -
描述
小鼠单克隆抗体[2A1-9A4] to CIP2A -
宿主
Mouse -
经测试应用
适用于: WB, IPmore details -
种属反应性
与反应: Mouse, Human -
免疫原
Recombinant fragment corresponding to Human CIP2A (N terminal).
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阳性对照
- WB: HeLa, 293T, Jurkat and TCMK-1 whole cell lysates. IP: 293T
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 99% Tris buffered saline, 0.1% BSA
pH 7-8 -
Concentration information loading...
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纯度
IgG fraction -
纯化说明
The antibody was purified using a mouse immunoglobulin binding ligand. -
克隆
单克隆 -
克隆编号
2A1-9A4 -
同种型
IgG1 -
轻链类型
kappa -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab128179于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/5000 - 1/25000. Predicted molecular weight: 102 kDa.
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IP |
Use at 2-5 µg/mg of lysate.
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说明 |
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WB
1/5000 - 1/25000. Predicted molecular weight: 102 kDa. |
IP
Use at 2-5 µg/mg of lysate. |
靶标
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功能
Oncoprotein that inhibits PP2A and stabilizes MYC in human malignancies. Promotes anchorage-independent cell growth and tumor formation. -
组织特异性
Expressed at low levels in most of the tissues. Overexpressed in head-and-neck squamous cell carcinomas (HNSCC). Present in liver cancer cells (at protein level). -
细胞定位
Membrane. Cytoplasm. Slightly concentrates in the perinuclear region. - Information by UniProt
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数据库链接
- Entrez Gene: 57650 Human
- Entrez Gene: 224171 Mouse
- Omim: 610643 Human
- SwissProt: Q8TCG1 Human
- SwissProt: Q8BWY9 Mouse
- Unigene: 591308 Human
- Unigene: 24491 Mouse
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别名
- Cancerous inhibitor of PP2A antibody
- Cancerous Inhibitor of Protein Phosphatase 2A antibody
- CIP2A antibody
see all
图片
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All lanes : Anti-CIP2A antibody [2A1-9A4] (ab128179) at 1/1000 dilution
Lane 1 : HeLa Whole cell lysate
Lane 2 : HEK293T Whole cell lysate
Lane 3 : Jurkat Whole cell lysate
Lane 4 : mouse TCMK-1 Whole cell lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : HRP-conjugated goat anti-mouse IgG
Predicted band size: 102 kDaChemiluminescence with an exposure time of 30 seconds.
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293T whole cell lysate (1 mg for IP, 20% of IP loaded) was used for immunoprecipitation with ab128179 at 3 µg/mg (lane 1) and control IgG (lane 2). Western blotting was performed using ab128179 at 1/1000 dilution. Detection by chemiluminescence was performed for 10 seconds.
数据表及文件
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Datasheet download
文献 (1)
ab128179 被引用在 1 文献中.
- Wei R et al. MicroRNA-218 inhibits the cell proliferation and migration in clear cell renal cell carcinoma through targeting cancerous inhibitor of protein phosphatase 2A. Oncol Lett 17:3211-3218 (2019). PubMed: 30867751