Anti-CIP29抗体(ab225694)
Key features and details
- Rabbit polyclonal to CIP29
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-CIP29抗体
参阅全部 CIP29 一抗 -
描述
兔多克隆抗体to CIP29 -
宿主
Rabbit -
经测试应用
适用于: WB, IPmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat, Rabbit, Horse, Chicken, Cow, Dog, Chimpanzee, Orangutan, Xenopus tropicalis
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免疫原
Synthetic peptide within Human CIP29 aa 160-210. The exact sequence is proprietary. (NP_149073.1).
Database link: P82979 -
阳性对照
- WB: HeLa, HEK-293T and Jurkat whole cell lysates. IP: HeLa whole cell lysate.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
ab225694 was affinity purified using an epitope specific to CIP29 immobilized on solid support. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab225694于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/2000 - 1/10000. Predicted molecular weight: 24 kDa.
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| IP |
Use at 2-10 µg/mg of lysate.
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| 说明 |
|---|
|
WB
1/2000 - 1/10000. Predicted molecular weight: 24 kDa. |
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IP
Use at 2-10 µg/mg of lysate. |
靶标
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功能
Binds both single-stranded and double-stranded DNA with higher affinity for the single-stranded form. Specifically binds to scaffold/matrix attachment region DNA. Also binds single-stranded RNA. Enhances RNA unwinding activity of DDX39A. May participate in important transcriptional or translational control of cell growth, metabolism and carcinogenesis. -
组织特异性
Low expression in spleen, liver, pancreas, testis, thymus, heart, and kidney. Increased levels are seen in hepatocellular carcinoma and pancreatic adenocarcinoma. -
序列相似性
Contains 1 SAP domain. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 425058 Chicken
- Entrez Gene: 467031 Chimpanzee
- Entrez Gene: 732539 Cow
- Entrez Gene: 474392 Dog
- Entrez Gene: 100058867 Horse
- Entrez Gene: 84324 Human
- Entrez Gene: 66118 Mouse
- Entrez Gene: 100173904 Orangutan
see all -
别名
- Cytokine induced protein of 29 kDa antibody
- Cytokine-induced protein of 29 kDa antibody
- HCC 1 antibody
see all
图片
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Western blot - Anti-CIP29 antibody (ab225694)All lanes : Anti-CIP29 antibody (ab225694)
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 15 µg
Lane 3 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Lane 4 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 24 kDa
Exposure time: 30 seconds -
Immunoprecipitation - Anti-CIP29 antibody (ab225694)CIP29 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab225694 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab225694 at 0.4 µg/ml.
Lane 1: ab225694 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.
Detection: Chemiluminescence with exposure time of 10 seconds.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab225694 尚未被引用在任何文献中。