重组Anti-cIAP1抗体[EPR4673] (ab108361)
![Western blot - Anti-cIAP1 antibody [EPR4673] (ab108361)](https://www.abcam.cn/ps/products/108/ab108361/Images/ab108361-397483-anti-ciap1-antibody-epr4673-western-blot-hela-lysates.jpg "Western blot - Anti-cIAP1 antibody [EPR4673] (ab108361)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4673] to cIAP1
- Suitable for: WB, IHC-P
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-cIAP1抗体[EPR4673]
参阅全部 cIAP1 一抗 -
描述
兔单克隆抗体[EPR4673] to cIAP1 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details
不适用于: Flow Cyt,ICC/IF or IP -
种属反应性
与反应: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: Jurkat, HeLa, HT-29, and HepG2 whole cell lysate (ab7900) IHC-P: Human spleen tissue
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, PBS, 0.05% BSA -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR4673 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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KO cell lines
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KO cell lysates
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab108361于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/1000 - 1/10000. Detects a band of approximately 70 kDa (predicted molecular weight: 70 kDa).
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| IHC-P |
1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
For purification use at 1/50 - 1/100 |
| 说明 |
|---|
|
WB
1/1000 - 1/10000. Detects a band of approximately 70 kDa (predicted molecular weight: 70 kDa). |
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IHC-P
1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. For purification use at 1/50 - 1/100 |
靶标
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功能
Apoptotic suppressor. The BIR motifs region interacts with TNF receptor associated factors 1 and 2 (TRAF1 and TRAF2) to form an heteromeric complex, which is then recruited to the tumor necrosis factor receptor 2 (TNFR2). -
组织特异性
Present in many fetal and adult tissues. Mainly expressed in adult skeletal muscle, thymus, testis, ovary, and pancreas, low or absent in brain and peripheral blood leukocytes. -
序列相似性
Belongs to the IAP family.
Contains 3 BIR repeats.
Contains 1 CARD domain.
Contains 1 RING-type zinc finger. -
细胞定位
Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 329 Human
- Omim: 601712 Human
- SwissProt: Q13490 Human
- Unigene: 696238 Human
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别名
- API 1 antibody
- API1 antibody
- Apoptosis inhibitor 1 antibody
see all
图片
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Western blot - Anti-cIAP1 antibody [EPR4673] (ab108361)All lanes : Anti-cIAP1 antibody [EPR4673] (ab108361) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : BIRC2 knockout HeLa cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 70 kDa
Observed band size: 70 kDaLanes 1- 2: Merged signal (red and green). Green - ab108361 observed at 70 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (ab8245) observed at 37 kDa.
ab108361 was shown to react with cIAP1 in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab265896 (knockout cell lysate ab257372) was used. Wild-type HeLa and BIRC2 knockout HeLa cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab108361 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-cIAP1 antibody [EPR4673] (ab108361)](https://www.abcam.cn/ps/products/108/ab108361/Images/ab108361-318891-anti-ciap1-antibody-epr4673-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-cIAP1 antibody [EPR4673] (ab108361)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human spleen tissue sections labeling cIAP1 with Purified ab108361 at 1:500 dilution (2.82 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain. -
![Western blot - Anti-cIAP1 antibody [EPR4673] (ab108361)](https://www.abcam.cn/ps/products/108/ab108361/Images/ab108361-269752-anti-ciap1-antibody-epr4673-western-blot.jpg)
Western blot - Anti-cIAP1 antibody [EPR4673] (ab108361)Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: cIAP1 knockout HAP1 cell lysate (20 µg)
Lane 3: HeLa cell lysate (20 µg)
Lane 4: Jurkat cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab108361 observed at 70 kDa. Red - loading control, ab8245, observed at 37 kDa.ab108361 was shown to specifically react with cIAP1 when cIAP1 knockout samples were used. Wild-type and cIAP1 knockout samples were subjected to SDS-PAGE. Ab108361 and ab8245 (loading control to GAPDH) were diluted at 1/1000 and 1/10,000 dilution respectively and incubated overnight at 4C. Blots were developed withGoat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-cIAP1 antibody [EPR4673] (ab108361)](https://www.abcam.cn/ps/products/108/ab108361/Images/ab108361-318919-anti-ciap1-antibody-epr4673-western-blot.jpg)
Western blot - Anti-cIAP1 antibody [EPR4673] (ab108361)Anti-cIAP1 antibody [EPR4673] (ab108361) at 1/2000 dilution (purified) + HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysates at 1/15 dilution
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/200000 dilution
Predicted band size: 70 kDa -
![Western blot - Anti-cIAP1 antibody [EPR4673] (ab108361)](https://www.abcam.cn/ps/products/108/ab108361/Images/ab108361-120993-anti-ciap1-antibody-epr4673-western-blot.jpg)
Western blot - Anti-cIAP1 antibody [EPR4673] (ab108361)All lanes : Anti-cIAP1 antibody [EPR4673] (ab108361) at 1/1000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : HT29 cell lysate
Lane 4 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 70 kDa
Observed band size: 70 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-cIAP1 antibody [EPR4673] (ab108361)](https://www.abcam.cn/ps/products/108/ab108361/Images/ab108361-120992-anti-ciap1-antibody-epr4673-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-cIAP1 antibody [EPR4673] (ab108361)ab108361, at 1/50, staining cIAP1 in paraffin-embedded Human spleen tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Anti-cIAP1 antibody [EPR4673] (ab108361)](https://www.abcam.cn/ps/products/108/ab108361/Images/ab108361-7-benefits-of-recombinant-antibodies.png)
Anti-cIAP1 antibody [EPR4673] (ab108361)
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (26)
ab108361 被引用在 26 文献中.
- Hayashi Y et al. Aberrant accumulation of NIK promotes tumor growth by dysregulating translation and post-translational modifications in breast cancer. Cancer Cell Int 23:57 (2023). PubMed: 37005661
- Deng QF et al. DNMT3A governs tyrosine kinase inhibitors responses through IAPs and in a cell senescence-dependent manner in non-small cell lung cancer. Am J Cancer Res 13:3517-3530 (2023). PubMed: 37693159
- Li D et al. Crotonylation sensitizes IAPi-induced disruption of latent HIV by enhancing p100 cleavage into p52. iScience 25:103649 (2022). PubMed: 35024584
- Huo J et al. BI 2536 induces gasdermin E-dependent pyroptosis in ovarian cancer. Front Oncol 12:963928 (2022). PubMed: 36016611
- Ren Y et al. Alternol Sensitizes Renal Carcinoma Cells to TRAIL-Induced Apoptosis. Front Pharmacol 12:560903 (2021). PubMed: 33841136