Anti-CHX10抗体(ab16141)
Key features and details
- Sheep polyclonal to CHX10
- Suitable for: WB
- Reacts with: Mouse, Rat
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
-
产品名称
Anti-CHX10抗体
参阅全部 CHX10 一抗 -
描述
羊多克隆抗体to CHX10 -
宿主
Sheep -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Mouse, Rat -
免疫原
Recombinant fragment corresponding to Human CHX10 aa 264-361 (C terminal).
Sequence:EAAAEKPEGERQALPKLDKMEQDERGPDAQAAISQEELRENSIAVLRAKA QEHSTKVLGTVSGPDSLARSTEKPEEEEAMDEDRPAERLSPPQLEDMA
Database link: P58304 -
常规说明
Chx10 is a 46kDa homeodomain protein of the paired-like class that is essential for development of the mammalian eye. Mutations in Chx10 cause microphthalmia, a cause of congenital blindness in humans, and the ocular retardation (or) phenotype in mice. In the developing mouse retina Chx10 is expressed in retinal progenitors, while in the mature retina, Chx10 expression becomes restricted to bipolar neurons. Concurrent with these expression patterns, the Chx10-/- (or) retina is thin due to a defect in proliferation of retinal progenitors, and lacks bipolar neurons. Chx10 is also expressed in the developing brainstem, thalamus, and spinal cord.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.08% Sodium azide
Constituent: PBS -
Concentration information loading... -
纯度
Ammonium Sulphate Precipitation -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
ChIP Related Products
-
Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab16141于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 46 kDa.
|
| 说明 |
|---|
|
WB
Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 46 kDa. |
靶标
-
功能
Plays a significant role in the specification and morphogenesis of the sensory retina. May also participate in the development of the cells of the inner nuclear layer, particularly bipolar cells. -
组织特异性
Abundantly expressed in retinal neuroblasts during eye development and in the inner nuclear layer of the adult retina. Within this layer, expression is stronger in the outer margin where bipolar cells predominate. -
疾病相关
Defects in VSX2 are the cause of microphthalmia isolated type 2 (MCOP2) [MIM:610093]; also known as isolated clinical anophthalmia. Microphthalmia is a clinically heterogeneous disorder of eye formation, ranging from small size of a single eye to complete bilateral absence of ocular tissues. Ocular abnormalities like opacities of the cornea and lens, scaring of the retina and choroid, cataractand other abnormalities like cataract may also be present.
Defects in VSX2 are the cause of microphthalmia with cataracts and iris abnormalities (MCOPCTI) [MIM:610092].
Defects in VSX2 are the cause of microphthalmia isolated with coloboma type 3 (MCOPCB3) [MIM:610092]; also known as isolated colobomatous microphthalmia 3. Ocular colobomas are a set of malformations resulting from abnormal morphogenesis of the optic cup and stalk, and the fusion of the fetal fissure (optic fissure). -
序列相似性
Belongs to the paired homeobox family.
Contains 1 CVC domain.
Contains 1 homeobox DNA-binding domain. -
细胞定位
Nucleus. - Information by UniProt
-
数据库链接
- Entrez Gene: 12677 Mouse
- Entrez Gene: 171360 Rat
- SwissProt: Q61412 Mouse
- Unigene: 4405 Mouse
-
别名
- C elegans ceh 10 homeo domain containing homolog antibody
- Ceh 10 homeo domain containing homolog (C. elegans) antibody
- Ceh 10 homeo domain containing homolog antibody
see all
图片
-
Western blot - Anti-CHX10 antibody (ab16141)
Western blot analysis using ab16141 at 1 µg/ml on rat liver (A), rat retina tissue lysate (B), mouse liver (C) and mouse retina tissue lysate (D).
实验方案
数据表及文件
-
SDS download
-
Datasheet download
文献 (25)
ab16141 被引用在 25 文献中.
- Chen HY et al. Reserpine maintains photoreceptor survival in retinal ciliopathy by resolving proteostasis imbalance and ciliogenesis defects. Elife 12:N/A (2023). PubMed: 36975211
- Ong ALC et al. Acquisition of neural fate by combination of BMP blockade and chromatin modification. iScience 26:107887 (2023). PubMed: 37771660
- Pimpinella S et al. Rabies Anterograde Monosynaptic Tracing Allows Identification of Postsynaptic Circuits Receiving Distinct Somatosensory Input. Neuroscience 491:75-86 (2022). PubMed: 35306143
- Hu S et al. Activation of oxytocin receptors in mouse GABAergic amacrine cells modulates retinal dopaminergic signaling. BMC Biol 20:205 (2022). PubMed: 36127701
- Zhong DJ et al. Adenosine A2A receptor antagonism protects against hyperoxia-induced retinal vascular loss via cellular proliferation. FASEB J 35:e21842 (2021). PubMed: 34418159