Anti-CHSS2抗体(ab224495)
Key features and details
- Rabbit polyclonal to CHSS2
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-CHSS2抗体
参阅全部 CHSS2 一抗 -
描述
兔多克隆抗体to CHSS2 -
宿主
Rabbit -
经测试应用
适用于: IHC-P, WB, ICC/IFmore details -
种属反应性
与反应: Human
预测可用于: Mouse
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免疫原
Recombinant fragment corresponding to Human CHSS2 aa 250-400.
Database link: Q8IZ52 -
阳性对照
- IHC-P: Human small intestine tissue. WB: CHSS2 overexpression HEK-293T cell lysate. ICC/IF: U-251 MG cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab224495于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| WB |
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 85 kDa.
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| ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100. |
| 说明 |
|---|
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IHC-P
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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WB
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 85 kDa. |
|
ICC/IF
Use a concentration of 0.25 - 2 µg/ml. Fixation/Permeabilization: PFA/Triton X-100. |
靶标
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相关性
CHSS2 belongs to the chondroitin N-acetylgalactosaminyltransferase family. It has both beta-1,3-glucuronic acid and beta-1,4-N-acetylgalactosamine transferase activity and transfers glucuronic acid (GlcUA) from UDP-GlcUA and N-acetylgalactosamine (GalNAc) from UDP-GalNAc to the non-reducing end of the elongating chondroitin polymer. CHSS2 is ubiquitous; highly expressed in pancreas, ovary, brain, heart, skeletal muscle, colon, kidney, liver, stomach, small intestine and placenta. -
细胞定位
Golgi apparatus, golgi stack membrane; Single pass type II membrane protein. -
数据库链接
- Entrez Gene: 79586 Human
- Entrez Gene: 74241 Mouse
- Omim: 610405 Human
- SwissProt: Q8IZ52 Human
- SwissProt: Q6IQX7 Mouse
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别名
- Chondroitin glucuronyltransferase II antibody
- Chondroitin polymerizing factor antibody
- Chondroitin sulfate synthase 2 antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CHSS2 antibody (ab224495)
Paraffin-embedded human small intestine tissue stained for CHSS2 using ab224495 at 1/200 dilution in immunohistochemical analysis.
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Western blot - Anti-CHSS2 antibody (ab224495)All lanes : Anti-CHSS2 antibody (ab224495)
Lane 1 : Vector only transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Lane 2 : CHSS2 overepression HEK-293T cell lysate (Co-expressed with a C-terminal myc-DDK tag, ~3.1 kDa)
Developed using the ECL technique.
Predicted band size: 85 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-CHSS2 antibody (ab224495)PFA-fixed, Triton X-100 permeabilized U-251 MG (human brain glioma cell line) cells stained for CHSS2 (green) using ab224495 at 4 µg/ml in ICC/IF.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab224495 被引用在 2 文献中.
- Li WW et al. Bioinformatics and Experimental Analysis of the Prognostic and Predictive Value of the CHPF Gene on Breast Cancer. Front Oncol 12:856712 (2022). PubMed: 35372047
- Xu Q et al. Chondroitin polymerizing factor (CHPF) contributes to malignant proliferation and migration of hepatocellular carcinoma cells. Biochem Cell Biol 98:362-369 (2020). PubMed: 32383983