重组Anti-Chromogranin C/SGII抗体[EPR23876-52] (ab275019)
![Western blot - Anti-Chromogranin C/SGII antibody [EPR23876-52] (ab275019)](https://www.abcam.cn/ps/products/275/ab275019/Images/ab275019-11-anti-chromogranin-csgii-antibody-epr2387652-western-blot-lysate.jpg "Western blot - Anti-Chromogranin C/SGII antibody [EPR23876-52] (ab275019)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23876-52] to Chromogranin C/SGII
- Suitable for: IHC-P, ICC/IF, Flow Cyt (Intra), WB, IHC-Fr
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-Chromogranin C/SGII抗体[EPR23876-52]
参阅全部 Chromogranin C/SGII 一抗 -
描述
兔单克隆抗体[EPR23876-52] to Chromogranin C/SGII -
宿主
Rabbit -
经测试应用
适用于: IHC-P, ICC/IF, Flow Cyt (Intra), WB, IHC-Frmore details
不适用于: IP -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: SH-SY-5Y, Neuro-2a, Beta-TC-6, GH3 and PC-12 whole cell lysates; Mouse hypothalamus tisue lysate; Rat hypothalamus tissue lysate. IHC-P: Human pancreas tissue; Mouse pancreas tissue; Rat pancreas tissue. IHC-Fr: Mouse pancreas tissue; Rat pancreas tissue. ICC/IF: PC-12 and Beta-TC-6 cells. Flow Cyt (intra): PC-12 and Beta-TC-6 cells.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR23876-52 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab275019于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
1/2000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
|
|
| ICC/IF |
1/50.
|
|
| Flow Cyt (Intra) |
1/500.
|
|
| WB |
1/1000. Predicted molecular weight: 71 kDa.
|
|
| IHC-Fr |
1/500.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). |
| 说明 |
|---|
|
IHC-P
1/2000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
|
ICC/IF
1/50. |
|
Flow Cyt (Intra)
1/500. |
|
WB
1/1000. Predicted molecular weight: 71 kDa. |
|
IHC-Fr
1/500. Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). |
靶标
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功能
Secretogranin-2 is a neuroendocrine secretory granule protein, which is the precursor for biologically active peptides. -
序列相似性
Belongs to the chromogranin/secretogranin protein family. -
细胞定位
Secreted. Neuroendocrine and endocrine secretory granules. - Information by UniProt
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数据库链接
- Entrez Gene: 7857 Human
- Entrez Gene: 20254 Mouse
- Entrez Gene: 24765 Rat
- Omim: 118930 Human
- SwissProt: P13521 Human
- SwissProt: Q03517 Mouse
- SwissProt: P10362 Rat
- Unigene: 516726 Human
see all -
别名
- CHGC antibody
- Chromogranin C antibody
- Chromogranin-C antibody
see all
图片
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Western blot - Anti-Chromogranin C/SGII antibody [EPR23876-52] (ab275019)All lanes : Anti-Chromogranin C/SGII antibody [EPR23876-52] (ab275019) at 1/1000 dilution
Lane 1 : Mouse hypothalamus tissue lysate
Lane 2 : Rat hypothalamus tissue lysate
Lysates/proteins at 40 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/20000 dilution
Predicted band size: 71 kDaBlocking and dilution buffer: 5% NFDM/TBST.
Exposure times: Lane 1: 26 seconds; Lane 2: 48 seconds.
Chromogranin C/SGII is proteolytically processed to small fragments. 81-kDa, 22 to 48 kDa forms are observed. The molecular weight observed is consistent with what has been described in the literature (PMID: 2282535, 8492910, 16357044, 7883050).
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![Immunocytochemistry/ Immunofluorescence - Anti-Chromogranin C/SGII antibody [EPR23876-52] (ab275019)](https://www.abcam.cn/ps/products/275/ab275019/Images/ab275019-4-anti-chromogranin-csgii-antibody-epr2387652-immunocytochemistry-pc12.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Chromogranin C/SGII antibody [EPR23876-52] (ab275019)Immunofluorescent analysis of 100% methanol-fixed PC-12 cells labelling Chromogranin C/SGII with ab275019 at 1/50 (10.68 ug/ml) dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in PC-12 cell line. is observed. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Chromogranin C/SGII antibody [EPR23876-52] (ab275019)](https://www.abcam.cn/ps/products/275/ab275019/Images/ab275019-3-anti-chromogranin-csgii-antibody-epr2387652-immunohistochemistry-rat-pancreas.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Chromogranin C/SGII antibody [EPR23876-52] (ab275019)Immunohistochemical analysis of paraffin-embedded Rat pancreas tissue labeling Chromogranin C/SGII with ab275019 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining in pancreatic islet (PMID: 30690892). The section was incubated with ab275019 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.
-
![Western blot - Anti-Chromogranin C/SGII antibody [EPR23876-52] (ab275019)](https://www.abcam.cn/ps/products/275/ab275019/Images/ab275019-10-anti-chromogranin-csgii-antibody-epr2387652-western-blot-epithelial.jpg)
Western blot - Anti-Chromogranin C/SGII antibody [EPR23876-52] (ab275019)All lanes : Anti-Chromogranin C/SGII antibody [EPR23876-52] (ab275019) at 1/1000 dilution
Lane 1 : SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate
Lane 2 : Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate
Lane 3 : Beta-TC-6 (mouse pancreas insulinoma beta cell) whole cell lysate
Lane 4 : GH3 (rat pituitary epithelial) whole cell lysate
Lane 5 : PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate
Lysates/proteins at 40 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/20000 dilution
Predicted band size: 71 kDaBlocking and dilution buffer: 5% NFDM/TBST.
Exposure times: Lane 1: 37 seconds; Lane 2: 15 seconds; Lane3-4: 5.5 seconds; Lane5: 1 second.
Chromogranin C/SGII is proteolytically processed to small fragments. 81-kDa, 55-kDa, 22 to 48 kDa forms are observed. The molecular weight observed is consistent with what has been described in the literature (PMID: 2282535, 8492910, 16357044, 7883050).
-
![Immunocytochemistry/ Immunofluorescence - Anti-Chromogranin C/SGII antibody [EPR23876-52] (ab275019)](https://www.abcam.cn/ps/products/275/ab275019/Images/ab275019-5-anti-chromogranin-csgii-antibody-epr2387652-immunocytochemistry-betatc6.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Chromogranin C/SGII antibody [EPR23876-52] (ab275019)Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Beta-TC-6 cells labelling Chromogranin C/SGII with ab275019 at 1/50 (10.68 ug/ml) dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in Beta-TC-6 cell line. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Chromogranin C/SGII antibody [EPR23876-52] (ab275019)](https://www.abcam.cn/ps/products/275/ab275019/Images/ab275019-2-anti-chromogranin-csgii-antibody-epr2387652-immunohistochemistry-mouse-pancreas.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Chromogranin C/SGII antibody [EPR23876-52] (ab275019)Immunohistochemical analysis of paraffin-embedded Mouse pancreas tissue labeling Chromogranin C/SGII with ab275019 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining in mouse pancreatic islet (PMID: 30690892). The section was incubated with ab275019 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.
-
![Immunohistochemistry (Frozen sections) - Anti-Chromogranin C/SGII antibody [EPR23876-52] (ab275019)](https://www.abcam.cn/ps/products/275/ab275019/Images/ab275019-9-anti-chromogranin-csgii-antibody-epr2387652-immunohistochemistry-frozen-rat-pancreas.jpg)
Immunohistochemistry (Frozen sections) - Anti-Chromogranin C/SGII antibody [EPR23876-52] (ab275019)Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat pancreas tissue labeling Chromogranin C/SGII with ab275019 at 1/500 (1.068 ug/ml) dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). Positive staining on rat pancreatic islet is observed. The nuclear counterstain was DAPI (Blue). Insulin was counterstained using ab6995 at 1/1000 dilution, followed by ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) at 1/1000 dilution (Red).
Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)at 1/1000 dilution.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
-
![Flow Cytometry (Intracellular) - Anti-Chromogranin C/SGII antibody [EPR23876-52] (ab275019)](https://www.abcam.cn/ps/products/275/ab275019/Images/ab275019-7-anti-chromogranin-csgii-antibody-epr2387652-flow-cytometry-pc12.jpg)
Flow Cytometry (Intracellular) - Anti-Chromogranin C/SGII antibody [EPR23876-52] (ab275019)Intracellular flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized PC-12 (Rat adrenal gland pheochromocytoma) cells labelling Chromogranin C/SGII with ab275019 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.
-
![Immunohistochemistry (Frozen sections) - Anti-Chromogranin C/SGII antibody [EPR23876-52] (ab275019)](https://www.abcam.cn/ps/products/275/ab275019/Images/ab275019-8-anti-chromogranin-csgii-antibody-epr2387652-immunohistochemistry-frozen-mouse-pancreas.jpg)
Immunohistochemistry (Frozen sections) - Anti-Chromogranin C/SGII antibody [EPR23876-52] (ab275019)Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse pancreas tissue labeling Chromogranin C/SGII with ab275019 at 1/500 (1.068 ug/ml) dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). Positive staining on mouse pancreatic islet is observed. Insulin was counterstained using ab6995 at 1/1000 dilution, followed by ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) at 1/1000 dilution (Red).The nuclear counterstain was DAPI (Blue).
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
-
![Flow Cytometry (Intracellular) - Anti-Chromogranin C/SGII antibody [EPR23876-52] (ab275019)](https://www.abcam.cn/ps/products/275/ab275019/Images/ab275019-6-anti-chromogranin-csgii-antibody-epr2387652-flow-cytometry-betatc6.jpg)
Flow Cytometry (Intracellular) - Anti-Chromogranin C/SGII antibody [EPR23876-52] (ab275019)Intracellular flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Beta-TC-6 (Mouse pancreas insulinoma beta cell) cells labelling Chromogranin C/SGII with ab275019 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Chromogranin C/SGII antibody [EPR23876-52] (ab275019)](https://www.abcam.cn/ps/products/275/ab275019/Images/ab275019-1-anti-chromogranin-csgii-antibody-epr2387652-immunohistochemistry-human-pancreas.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Chromogranin C/SGII antibody [EPR23876-52] (ab275019)Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling Chromogranin C/SGII with ab275019 at 1/2000 (0.267 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining in human pancreatic islet (PMID: 30690892)The section was incubated with ab275019 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
-
![Anti-Chromogranin C/SGII antibody [EPR23876-52] (ab275019)](https://www.abcam.cn/ps/products/275/ab275019/Images/ab275019-12-benefits-of-recombinant-antibodies.png)
Anti-Chromogranin C/SGII antibody [EPR23876-52] (ab275019)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (0)
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