重组Anti-Chromogranin A抗体[EP1031Y] (ab52983)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Chromogranin A antibody [EP1031Y] (ab52983)](https://www.abcam.cn/ps/products/52/ab52983/Images/ab52983-65557-anti-chromogranin-a-antibody-ep1031y-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Chromogranin A antibody [EP1031Y] (ab52983)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1031Y] to Chromogranin A
- Suitable for: WB, IHC-P
- Reacts with: Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-Chromogranin A抗体[EP1031Y]
参阅全部 Chromogranin A 一抗 -
描述
兔单克隆抗体[EP1031Y] to Chromogranin A -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details
不适用于: Flow Cyt or ICC/IF -
种属反应性
与反应: Rat, Human -
免疫原
Synthetic peptide within Human Chromogranin A (C terminal). The exact sequence is proprietary.
(Peptide available asab138920) -
阳性对照
- PC12 cytoplasmic cell lysate Human pancreatic tissue
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
解离常数（KD）
KD = 6.90 x 10 -12 M Learn more about KD -
存储溶液
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EP1031Y -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab52983于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/1e+006. Detects a band of approximately 80-90 kDa (predicted molecular weight: 51 kDa).
|
|
| IHC-P |
1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
|
| 说明 |
|---|
|
WB
1/1e+006. Detects a band of approximately 80-90 kDa (predicted molecular weight: 51 kDa). |
|
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
靶标
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功能
Pancreastatin strongly inhibits glucose induced insulin release from the pancreas. -
序列相似性
Belongs to the chromogranin/secretogranin protein family. -
翻译后修饰
Sulfated on tyrosine residues and/or contains sulfated glycans.
O-glycosylated with core 1 or possibly core 8 glycans. -
细胞定位
Secreted. Neuroendocrine and endocrine secretory granules. - Information by UniProt
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数据库链接
- Entrez Gene: 1113 Human
- Entrez Gene: 24258 Rat
- Omim: 118910 Human
- SwissProt: P10645 Human
- SwissProt: P10354 Rat
- Unigene: 150793 Human
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形式
According to the Swiss-Prot database, this protein has many different forms which correspond to various molecular weights; thus the possible variation from the predicted molecular weight of 51kDa. -
别名
- beta Granin antibody
- betagranin (N-terminal fragment of chromogranin A) antibody
- catestatin antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Chromogranin A antibody [EP1031Y] (ab52983)
ab52983, at a 1/250 dilution, staining Human Chromogranin A in Pancreas using Immunohistochemistry, Paraffin Emebedded tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Western blot - Anti-Chromogranin A antibody [EP1031Y] (ab52983)](https://www.abcam.cn/ps/products/52/ab52983/Images/ab52983-65558-anti-chromogranin-a-antibody-ep1031y-western-blot.jpg)
Western blot - Anti-Chromogranin A antibody [EP1031Y] (ab52983)Anti-Chromogranin A antibody [EP1031Y] (ab52983) at 1/1000000 dilution + PC12 cell lysate at 10 µg
Secondary
goat anti-rabbit HRP labelled at 1/2000 dilution
Predicted band size: 51 kDa
Observed band size: 80-90 kDa why is the actual band size different from the predicted? -
![OI-RD Scanning - Anti-Chromogranin A antibody [EP1031Y] (ab52983)](https://www.abcam.cn/ps/products/52/ab52983/Images/ab52983-65556-anti-chromogranin-a-antibody-ep1031y.jpg)
OI-RD Scanning - Anti-Chromogranin A antibody [EP1031Y] (ab52983) -
![Anti-Chromogranin A antibody [EP1031Y] (ab52983)](https://www.abcam.cn/ps/products/52/ab52983/Images/ab52983-4-benefits-of-recombinant-antibodies.png)
Anti-Chromogranin A antibody [EP1031Y] (ab52983)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (4)
ab52983 被引用在 4 文献中.
- Rao VKS et al. Multiple roles for peptidylglycine a-amidating monooxygenase in the response to hypoxia. J Cell Physiol N/A:N/A (2021). PubMed: 34061983
- Hu J et al. Pitfalls in the management of subglottic paragangliomas at unusual location: a case report and literature review. BMC Surg 21:340 (2021). PubMed: 34496808
- Thomsen SK et al. Type 2 diabetes risk alleles in PAM impact insulin release from human pancreatic ß-cells. Nat Genet 50:1122-1131 (2018). PubMed: 30054598
- Simpson PD et al. Striking Oxygen Sensitivity of the Peptidylglycine a-Amidating Monooxygenase (PAM) in Neuroendocrine Cells. J Biol Chem 290:24891-901 (2015). WB ; Human . PubMed: 26296884