AB74308

Anti-CHRAC-17抗体

Name: CHRAC-17抗体 (ab74308)| Abcam中文官网
Brand: Abcam Limited
SKU: AB74308
Price: 4514 CNY
Availability: InStock

Anti-CHRAC-17 antibody

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(1 Publication)

Rabbit Polyclonal CHRAC-17 antibody. Suitable for IHC-P, IP, WB and reacts with Mouse, Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human POLE3 aa 50 to C-terminus.

查看别名

CHRAC17, POLE3, DNA polymerase epsilon subunit 3, Arsenic-transactivated protein, Chromatin accessibility complex 17 kDa protein, DNA polymerase II subunit 3, DNA polymerase epsilon subunit p17, AsTP, CHRAC-17, HuCHRAC17

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CHRAC-17 antibody (AB74308)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CHRAC-17 antibody (AB74308)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma (left) and mouse teratoma (right) tissues labelling DNA Polymerase epsilon with ab74308 at 1/5000 (0.2µg/ml). Detection : DAB.

Unknown

Immunoprecipitation - Anti-CHRAC-17 antibody (AB74308)

Detection of human CHRAC-17 by immunoprecipitation of 1mg HeLa cells whole lysate, using ab74308 at 3μg/mg lysate (lane 1, 20% IP loaded) or a control IgG (lane 2, 20% IP loaded). The post-IP WB was performed using ab74308 at 1 μg/ml.

Detection : chemiluminescence with an exposure time of 30 seconds.

All lanes:

Immunoprecipitation - Anti-CHRAC-17 antibody (ab74308)

Predicted band size: 16 kDa

false

Unknown

Western blot - Anti-CHRAC-17 antibody (AB74308)

All lanes:

Western blot - Anti-CHRAC-17 antibody (ab74308) at 0.4 µg/mL

Lane 1:

Whole cell lysate from HeLa cells at 50 µg

Lane 2:

Whole cell lysate from HeLa cells at 15 µg

Lane 3:

Whole cell lysate from HeLa cells at 5 µg

Lane 4:

Whole cell lysate from HEK-293T cells at 50 µg

Predicted band size: 16 kDa

Observed band size: 18 kDa

false

关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

反应种属

Mouse, Human

应用

WB, IP, IHC-P

applications

免疫原

Synthetic Peptide within Human POLE3 aa 50 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9NRF9

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000 - 1/5000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000 - 1/5000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Bat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chinese hamster": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Guinea pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Immunogen

存储溶液

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: PBS, 1.815% Tris, 1.764% Sodium citrate

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Accessory component of the DNA polymerase epsilon complex (PubMed : 10801849). Participates in DNA repair and in chromosomal DNA replication (By similarity). Forms a complex with CHRAC1 and binds naked DNA, which is then incorporated into chromatin, aided by the nucleosome-remodeling activity of ISWI/SNF2H and ACF1 (PubMed : 10801849). Does not enhance nucleosome sliding activity of the ACF-5 ISWI chromatin remodeling complex (PubMed : 14759371).

See full target information POLE3

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Nucleic acids research 45:169-180 PubMed27679476

2016

H3K9me3 demethylase Kdm4d facilitates the formation of pre-initiative complex and regulates DNA replication.

Applications

Species

Unspecified reactive species

Rentian Wu,Zhiquan Wang,Honglian Zhang,Haiyun Gan,Zhiguo Zhang

View all publications

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For licensing inquiries, please contact partnerships@abcam.com

Anti-CHRAC-17抗体

Anti-CHRAC-17 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CHRAC-17 antibody (AB74308)

Immunoprecipitation - Anti-CHRAC-17 antibody (AB74308)

Western blot - Anti-CHRAC-17 antibody (AB74308)