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AB178850

Anti-Choline Acetyltransferase 抗体 [EPR16590]

Anti-Choline Acetyltransferase antibody [EPR16590]

5

(6 Reviews)

|

(54 Publications)

Anti-Choline Acetyltransferase antibody [EPR16590] (ab178850) is a rabbit monoclonal antibody detecting Choline Acetyltransferase in Western Blot, IHC-P. Suitable for Guinea pig, Mouse, Rabbit, Rat.

- Biophysical QC for unrivalled batch-batch consistency
- Over 50 publications

查看别名

Choline O-acetyltransferase, CHOACTase, ChAT, Choline acetylase, Chat

7 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Choline Acetyltransferase antibody [EPR16590] (AB178850)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Choline Acetyltransferase antibody [EPR16590] (AB178850)

Immunohistochemical analysis of paraffin-embedded Guinea Pig cerebral cortex tissue labeling Choline Acetyltransferase with ab178850 at 1/2000 followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Nucleus and cytoplasm staining on neurons of Guinea Pig cerebral cortex is observed. Counter stained with Hematoxylin.

Negative control : Using PBS instead of primary ab, secondary ab is prediluted HRP Polymer for Rabbit/Mouse IgG.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Western blot - Anti-Choline Acetyltransferase antibody [EPR16590] (AB178850)
  • WB

Supplier Data

Western blot - Anti-Choline Acetyltransferase antibody [EPR16590] (AB178850)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Choline Acetyltransferase antibody [EPR16590] (ab178850) at 1/1000 dilution

All lanes:

Rat brain lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Predicted band size: 82 kDa

Observed band size: 72 kDa

false

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Choline Acetyltransferase antibody [EPR16590] (AB178850)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Choline Acetyltransferase antibody [EPR16590] (AB178850)

Immunohistochemical analysis of paraffin-embedded Mouse cerebral cortex tissue labeling Choline Acetyltransferase with ab178850 at 1/2000 followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Nucleus and cytoplasm staining on neurons of Mouse cerebral cortex is observed. Counter stained with Hematoxylin.

Negative control : Using PBS instead of primary ab, secondary ab is prediluted HRP Polymer for Rabbit/Mouse IgG.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Choline Acetyltransferase antibody [EPR16590] (AB178850)
  • IHC-P

AbReview56980****

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Choline Acetyltransferase antibody [EPR16590] (AB178850)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse brain tissue sections labeling Choline Acetyltransferase with ab178850 at 1/2000 dilution. Cells were fixed in 10% normal buffered formalin, heat mediated antigen retrieval was performed using TE pH9. A donkey anti-rabbit Alexa Fluor 647 was used as the secondary antibody at 1/300 dilution. Green was a false colour used to represent the Alexa Fluor 647 fluorescent signal.

This image is courtesy of an anonymous abreview.

Western blot - Anti-Choline Acetyltransferase antibody [EPR16590] (AB178850)
  • WB

Supplier Data

Western blot - Anti-Choline Acetyltransferase antibody [EPR16590] (AB178850)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Choline Acetyltransferase antibody [EPR16590] (ab178850) at 1/2000 dilution

All lanes:

Mouse brain tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 82 kDa

Observed band size: 72 kDa

false

Exposure time: 3min

Western blot - Anti-Choline Acetyltransferase antibody [EPR16590] (AB178850)
  • WB

Supplier Data

Western blot - Anti-Choline Acetyltransferase antibody [EPR16590] (AB178850)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Choline Acetyltransferase antibody [EPR16590] (ab178850) at 1/5000 dilution

Lane 1:

Guinea Pig brain lysate at 20 µg

Lane 2:

Guinea Pig cerebellum lysate at 20 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 82 kDa

Observed band size: 72 kDa

false

Western blot - Anti-Choline Acetyltransferase antibody [EPR16590] (AB178850)
  • WB

Supplier Data

Western blot - Anti-Choline Acetyltransferase antibody [EPR16590] (AB178850)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Choline Acetyltransferase antibody [EPR16590] (ab178850) at 1/1000 dilution

All lanes:

Rabbit brain lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 82 kDa

Observed band size: 72 kDa

false

不同偶联物与剂型 (7)

  • Carrier free

    Anti-Choline Acetyltransferase antibody [EPR16590] - BSA and Azide free

  • 603 Alexa Fluor® 568

    Alexa Fluor® 568 Anti-Choline Acetyltransferase antibody [EPR16590]

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-Choline Acetyltransferase antibody [EPR16590]

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-Choline Acetyltransferase antibody [EPR16590]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-Choline Acetyltransferase antibody [EPR16590]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-Choline Acetyltransferase antibody [EPR16590]

  • 775 Alexa Fluor® 750

    Alexa Fluor® 750 Anti-Choline Acetyltransferase antibody [EPR16590]

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR16590

亚型

IgG

不含载体蛋白

No

反应种属

Mouse, Rat, Rabbit, Guinea pig

应用

IHC-P, WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/2000", "IHCP-species-notes": "<p><a href='/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Guinea pig": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/2000", "IHCP-species-notes": "<p><a href='/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." }, "Rabbit": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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为什么推荐这个？

我们推荐这个产品，因为它经常被用于相同的实验或相关的研究。

我们建议您务必查阅产品说明书，以确认其是否符合您的实验需求；若有疑问，也可联系我们的技术团队获取协助。

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产品详情

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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Protein A
存储溶液
Preservative: 0.01% Sodium azide Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
运输条件
Blue Ice
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Choline Acetyltransferase (ChAT) also known as EC 2.3.1.6 acts as the enzyme responsible for synthesizing the neurotransmitter acetylcholine. It catalyzes the transfer of an acetyl group from acetyl-CoA to choline to form acetylcholine. ChAT is a protein with an approximate mass of 70 kDa and is expressed in cholinergic neurons throughout the central and peripheral nervous systems. High expression is observed in regions such as the basal forebrain and neuromuscular junctions which are important for cholinergic signaling.
Biological function summary

The enzyme plays an important role in cholinergic conduction by producing acetylcholine which functions in synaptic transmission. It does not function as part of a larger complex but its activity directly influences cholinergic synapses. Choline acetyltransferase function ensures the synthesis and availability of acetylcholine for release into the synaptic cleft initiating muscle contraction and modulating activity in the central nervous system.

Pathways

Choline acetyltransferase is critical in pathways governing neurotransmitter release such as the cholinergic synapse pathway. It interacts functionally with other proteins within this pathway including acetylcholinesterase which breaks down acetylcholine in the synaptic cleft. This interaction ensures proper neurotransmitter regulation and recycling maintaining synapse function and communication within neuronal circuits.

Alterations in choline acetyltransferase activity relate to neurological disorders including Alzheimer's disease and myasthenia gravis. In Alzheimer's disease reduced choline acetyltransferase function leads to decreased acetylcholine synthesis contributing to the cognitive decline observed in patients. In myasthenia gravis acetylcholine receptors are impaired but effective choline acetyltransferase activity is still necessary to manage neurotransmitter levels. A potential therapeutic angle involves targeting related proteins that exacerbate these conditions to support acetylcholine levels.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses.
See full target information Chat
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文献 (54)

Recent publications for all applications. Explore the full list and refine your search

Communications biology 6:1145 PubMed37950055

2023

Repeated multi-domain cognitive training prevents cognitive decline, anxiety and amyloid pathology found in a mouse model of Alzheimer disease.

Applications

Unspecified application

Species

Unspecified reactive species

Jogender Mehla,Scott H Deibel,Hadil Karem,Nancy S Hong,Shakhawat R Hossain,Sean G Lacoursiere,Robert J Sutherland,Majid H Mohajerani,Robert J McDonald

Journal of neuroinflammation 20:158 PubMed37403174

2023

Caudal DMN neurons innervate the spleen and release CART peptide to regulate neuroimmune function.

Applications

Unspecified application

Species

Unspecified reactive species

Nobuhide Kobori,Anthony N Moore,John B Redell,Pramod K Dash

iScience 26:106954 PubMed37275532

2023

SARS-CoV-2 S1 protein causes brain inflammation by reducing intracerebral acetylcholine production.

Applications

Unspecified application

Species

Unspecified reactive species

Naomi Oka,Kazuya Shimada,Azusa Ishii,Nobuyuki Kobayashi,Kazuhiro Kondo

eLife 12: PubMed37102362

2023

Cholinergic and noradrenergic axonal activity contains a behavioral-state signal that is coordinated across the dorsal cortex.

Applications

Unspecified application

Species

Unspecified reactive species

Lindsay Collins,John Francis,Brett Emanuel,David A McCormick

Frontiers in aging neuroscience 15:1174341 PubMed37181622

2023

Polypyrimidine tract binding protein knockdown reverses depression-like behaviors and cognition impairment in mice with lesioned cholinergic neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Yiying Zhou,Ke Zhang,Fangmin Wang,Jiali Chen,Shanshan Chen,Manqing Wu,Miaojun Lai,Yisheng Zhang,Wenhua Zhou

Acta pharmaceutica Sinica. B 13:577-597 PubMed36873166

2023

Honokiol alleviated neurodegeneration by reducing oxidative stress and improving mitochondrial function in mutant SOD1 cellular and mouse models of amyotrophic lateral sclerosis.

Applications

Unspecified application

Species

Unspecified reactive species

Yujun Zhou,Jingshu Tang,Jiaqi Lan,Yong Zhang,Hongyue Wang,Qiuyu Chen,Yuying Kang,Yang Sun,Xinhong Feng,Lei Wu,Hongtao Jin,Shizhong Chen,Ying Peng

Brain sciences 13: PubMed36831801

2023

Immunohistochemical Distribution and Neurochemical Characterization of Huntingtin-Associated Protein 1 Immunoreactive Neurons in the Adult Mouse Lingual Ganglia.

Applications

Unspecified application

Species

Unspecified reactive species

Md Nabiul Islam,Yoshinori Sakurai,Yurie Hiwaki,Abu Md Mamun Tarif,Marya Afrin,Mirza Mienur Meher,Kanako Nozaki,Koh Hei Masumoto,Akie Yanai,Mir Rubayet Jahan,Koh Shinoda

Nature communications 14:344 PubMed36670126

2023

Sensory nerve niche regulates mesenchymal stem cell homeostasis via FGF/mTOR/autophagy axis.

Applications

Unspecified application

Species

Unspecified reactive species

Fei Pei,Li Ma,Junjun Jing,Jifan Feng,Yuan Yuan,Tingwei Guo,Xia Han,Thach-Vu Ho,Jie Lei,Jinzhi He,Mingyi Zhang,Jian-Fu Chen,Yang Chai

Neuronal signaling 7:NS20220098 PubMed36743438

2023

imaging of axonal transport in peripheral nerves of rodent forelimbs.

Applications

Unspecified application

Species

Unspecified reactive species

Qiuhan Lang,Giampietro Schiavo,James N Sleigh

Frontiers in neuroscience 16:1065897 PubMed36590290

2022

Inhibition by rno-circRNA-013017 of the apoptosis of motor neurons in anterior horn and descending axonal degeneration in rats after traumatic spinal cord injury.

Applications

Unspecified application

Species

Unspecified reactive species

Chuan Qin,Yi Liu,Pei-Pei Xu,Xin Zhang,Zuliyaer Talifu,Jia-Yi Liu,Ying-Li Jing,Fan Bai,Li-Xi Zhao,Yan Yu,Feng Gao,Jian-Jun Li
View all publications
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Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。
详情请参阅我们的条款与条件。
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