Key features and details
- Rabbit polyclonal to Cholera Toxin
- Suitable for: ELISA
- Isotype: IgG
参阅全部 Cholera Toxin 一抗
描述兔多克隆抗体to Cholera Toxin
经测试应用适用于: ELISAmore details
种属反应性与反应: Vibrio cholerae
Purified Choleragenoid (beta subunit).
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存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Preservative: 0.1% Sodium azide
Constituent: 0.0268% PBS
Concentration information loading...
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Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
相关性The holotoxin (choleragen) consists of a pentameric ring of B subunits whose central pore is occupied by the A subunit. The A subunit contains two chains, A1 and A2, linked by a disulfide bridge. The B subunit pentameric ring directs the A subunit to its target by binding to the GM1 gangliosides present on the surface of the intestinal epithelial cells. It can bind five GM1 gangliosides. It has no toxic activity by itself. After binding to gangliosides GM1 in lipid rafts, through the subunit B pentamer, the holotoxin and the gangliosides are internalized. The holotoxin remains bound to GM1 until arrival in the ER. The A subunit has previously been cleaved in the intestinal lumen but the A1 and A2 chains have remained associated. In the ER, the A subunit disulfide bridge is reduced, the A1 chain is unfolded by the PDI and disassembled from the rest of the toxin. Then, the membrane-associated ER oxidase ERO1 oxidizes PDI, which releases the unfolded A1 chain. The next step is the retrotranslocation of A1 into the cytosol. This might be mediated by the protein-conducting pore SEC61. Upon arrival in the cytosol, A1 refolds and avoids proteasome degradation. In one way or another, A1 finally reaches its target and induces toxicity.
- ctxA antibody
- Cholera enterotoxin B chain antibody
- Cholera enterotoxin gamma chain antibody
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.