重组Anti-Cholera enterotoxin subunit B抗体[EPR27372-4] - BSA and Azide free (ab317739)
![Western blot - Anti-Cholera enterotoxin subunit B antibody [EPR27372-4] - BSA and Azide free (ab317739)](https://www.abcam.cn/ps/products/317/ab317739/Images/ab317739-542263-anti-cholera-enterotoxin-subunit-b-antibody-epr27372-4-western-blot-293t-human-embryonic-kidney-cell-transfected-cell-lysate-ug.jpg "Western blot - Anti-Cholera enterotoxin subunit B antibody [EPR27372-4] - BSA and Azide free (ab317739)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR27372-4] to Cholera enterotoxin subunit B - BSA and Azide free
- Suitable for: WB, IHC-P, ICC/IF, IP, Indirect ELISA, Flow Cyt (Intra)
Related conjugates and formulations
概述
-
产品名称
Anti-Cholera enterotoxin subunit B抗体[EPR27372-4] - BSA and Azide free
参阅全部 Cholera enterotoxin subunit B 一抗 -
描述
兔单克隆抗体[EPR27372-4] to Cholera enterotoxin subunit B - BSA and Azide free -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, ICC/IF, IP, Indirect ELISA, Flow Cyt (Intra)more details -
种属反应性
与反应: Vibrio cholerae -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
阳性对照
- WB & IP : 293T transfected with a Cholera enterotoxin subunit B expression vector containing a EGFP tag, whole cell lysate. IHC-P: HEK-293T transfected with a Cholera enterotoxin subunit B expression vector containing a EGFP tag tissue. ICC/IF: 293T cell. Flow Cyt (Intra): 293T cells transfected with a Cholera enterotoxin subunit B expression vector containing an EGFP tag.
-
常规说明
ab317739 is the carrier-free version of ab317738.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
pH: 7.2
Constituent: 100% PBS -
无载体
是 -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR27372-4 -
同种型
IgG
相关产品
-
Alternative Versions
-
Compatible Secondaries
-
Isotype control
-
Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab317739于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use at an assay dependent concentration.
|
|
| IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
|
| ICC/IF |
Use at an assay dependent concentration.
|
|
| IP |
Use at an assay dependent concentration.
|
|
| Indirect ELISA |
Use at an assay dependent concentration.
|
|
| Flow Cyt (Intra) |
Use at an assay dependent concentration.
|
| 说明 |
|---|
|
WB
Use at an assay dependent concentration. |
|
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
|
ICC/IF
Use at an assay dependent concentration. |
|
IP
Use at an assay dependent concentration. |
|
Indirect ELISA
Use at an assay dependent concentration. |
|
Flow Cyt (Intra)
Use at an assay dependent concentration. |
靶标
-
别名
- Cholera enterotoxin B chain antibody
- Cholera enterotoxin gamma chain antibody
- Choleragenoid antibody
图片
-
Western blot - Anti-Cholera enterotoxin subunit B antibody [EPR27372-4] - BSA and Azide free (ab317739)All lanes : Anti-Cholera enterotoxin subunit B antibody [EPR27372-4] (ab317738) at 1/5000 dilution
Lane 1 : 293T (human embryonic kidney epithelial cell) transfected with an empty vector containing a EGFP tag, whole cell lysate
Lane 2 : 293T transfected with a Cholera enterotoxin subunit B expression vector containing a EGFP tag, whole cell lysate
Lysates/proteins at 2 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Observed band size: 40 kDa why is the actual band size different from the predicted?
Exposure time: 180 secondsThis data was developed using ab317738, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
In Western blot, Anti-GFP antibody [E385] (ab32146) staining at 1/10000 dilution.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cholera enterotoxin subunit B antibody [EPR27372-4] - BSA and Azide free (ab317739)](https://www.abcam.cn/ps/products/317/ab317739/Images/ab317739-2-anti-cholera-enterotoxin-subunit-b-antibody-epr27372-4-immunohistochemistry-a-hek-293t-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cholera enterotoxin subunit B antibody [EPR27372-4] - BSA and Azide free (ab317739)This data was developed using ab317738, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded (A) HEK-293T (human epithelial cell line from embryonic kidney) transfected with a Cholera enterotoxin subunit B expression vector containing a EGFP tag. (B) HEK-293T transfected with empty vector containing a EGFP tag. tissue labeling Cholera enterotoxin subunit B with ab317738 at 1/2000 (0.265 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on (A) HEK-293T (human epithelial cell line from embryonic kidney) transfected with a Cholera enterotoxin subunit B expression vector containing a EGFP tag, negative staining on (B) HEK-293T transfected with empty vector containing a EGFP tag. The section was incubated with ab317738 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
-
![Immunocytochemistry/ Immunofluorescence - Anti-Cholera enterotoxin subunit B antibody [EPR27372-4] - BSA and Azide free (ab317739)](https://www.abcam.cn/ps/products/317/ab317739/Images/ab317739-1-anti-cholera-enterotoxin-subunit-b-antibody-epr27372-4-immunocytochemistry-293t.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Cholera enterotoxin subunit B antibody [EPR27372-4] - BSA and Azide free (ab317739)This data was developed using ab317738, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (human embryonic kidney epithelial cell) cells labelling Cholera enterotoxin subunit B with ab317738 at 1/2000 (0.265 ug/ml) dilution, followed by ab150088 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed (ab150088) antibody at 1/1000 2 ug/ml dilution (Green).
Confocal image showing positive staining in 293T cells transfected with a Cholera enterotoxin subunit B expression vector (shown in magenta) containing a EGFP-tag® (shown in green). Nuclear DNA was labeled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).Secondary antibody only control: Secondary antibody is ab150088 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed (ab150088) at 1/1000 2 ug/ml dilution.
-
![Flow Cytometry (Intracellular) - Anti-Cholera enterotoxin subunit B antibody [EPR27372-4] - BSA and Azide free (ab317739)](https://www.abcam.cn/ps/products/317/ab317739/Images/ab317739-4-anti-cholera-enterotoxin-subunit-b-antibody-epr27372-4-flow-cytometry-isotype.jpg)
Flow Cytometry (Intracellular) - Anti-Cholera enterotoxin subunit B antibody [EPR27372-4] - BSA and Azide free (ab317739)This data was developed using ab317738, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Isotype (Left) / 293T cells transfected with a Cholera enterotoxin subunit B expression vector containing an EGFP tag (Middle) / 293T cells transfected with an empty expression vector containing an EGFP tag (Right) cells labelling Cholera enterotoxin subunit B with ab317738 at 1/5000 dilution (0.01 ug)/Middle and Right (Red) compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
Goat anti-Rabbit IgG (Alexa Fluor® 647, ab150083) at 1/5000 dilution was used as the secondary antibody.
-
![Immunoprecipitation - Anti-Cholera enterotoxin subunit B antibody [EPR27372-4] - BSA and Azide free (ab317739)](https://www.abcam.cn/ps/products/317/ab317739/Images/ab317739-3-anti-cholera-enterotoxin-subunit-b-antibody-epr27372-4-immunoprecipitation-293t.jpg)
Immunoprecipitation - Anti-Cholera enterotoxin subunit B antibody [EPR27372-4] - BSA and Azide free (ab317739)This data was developed using ab317738, the same antibody clone in a different buffer formulation.
Cholera enterotoxin subunit B was immunoprecipitated from 0.35 mg 293T (human embryonic kidney epithelial cell) transfected with a Cholera enterotoxin subunit B expression vector containing a EGFP tag, whole cell lysate with ab317738 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab317738 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: 293T (human embryonic kidney epithelial cell) transfected with a Cholera enterotoxin subunit B expression vector containing a EGFP tag, whole cell lysate
Lane 2: ab317738 IP in 293T (human embryonic kidney epithelial cell) transfected with a Cholera enterotoxin subunit B expression vector containing a EGFP tag, whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab317738 in 293T cells transfected with a Cholera enterotoxin subunit B expression vector containing a EGFP tag, whole cell lysateBlocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 10 seconds.
-
![Indirect ELISA - Anti-Cholera enterotoxin subunit B antibody [EPR27372-4] - BSA and Azide free (ab317739)](https://www.abcam.cn/ps/products/317/ab317739/Images/ab317739-5-anti-cholera-enterotoxin-subunit-b-antibody-epr27372-4-elisa-cholera-enterotoxin-subunit-b.jpg)
Indirect ELISA - Anti-Cholera enterotoxin subunit B antibody [EPR27372-4] - BSA and Azide free (ab317739)This data was developed using ab317738, the same antibody clone in a different buffer formulation.
Indirect ELISA analysis of ab317738 at 1000-0 ng/ml. The Secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1:2500 dilution.
Antigen: Cholera enterotoxin subunit B.
Antigen concentration: 1000 ng/ml
The recombinant protein of Cholera enterotoxin subunit B was made in house.
-
![Anti-Cholera enterotoxin subunit B antibody [EPR27372-4] - BSA and Azide free (ab317739)](https://www.abcam.cn/ps/products/317/ab317739/Images/ab317739-6-benefitsofrecombinantantibodies.jpg)
Anti-Cholera enterotoxin subunit B antibody [EPR27372-4] - BSA and Azide free (ab317739)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
-
Datasheet download
Certificate of Compliance
文献 (0)
ab317739 尚未被引用在任何文献中。
