Anti-CHMP4B / CHMP4C抗体(ab272638)
Key features and details
- Rabbit polyclonal to CHMP4B / CHMP4C
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-CHMP4B / CHMP4C抗体 -
描述
兔多克隆抗体to CHMP4B / CHMP4C -
宿主
Rabbit -
经测试应用
适用于: IHC-P, WBmore details -
种属反应性
与反应: Human -
免疫原
Recombinant fragment corresponding to Human CHMP4B/ CHMP4C aa 1-100.
Database link: Q9H444 -
阳性对照
- WB: HEK-293T lysate. IHC-P: Human kidney tissue, small intestine tissue, liver tissue.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab272638于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| WB |
Use a concentration of 0.04 - 0.4 µg/ml.
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| 说明 |
|---|
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IHC-P
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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WB
Use a concentration of 0.04 - 0.4 µg/ml. |
靶标
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相关性
CHMP4B and CHMP4C belong to the chromatin-modifying protein/charged multivesicular body protein (CHMP) family. These proteins are components of ESCRT-III (endosomal sorting complex required for transport III), a complex involved in degradation of surface receptor proteins and formation of endocytic multivesicular bodies (MVBs). Some CHMPs have both nuclear and cytoplasmic/vesicular distributions, and one such CHMP, CHMP1A (MIM 164010), is required for both MVB formation and regulation of cell cycle progression. -
细胞定位
Cytoplasmic -
数据库链接
- Entrez Gene: 128866 Human
- Entrez Gene: 92421 Human
- Omim: 610897 Human
- Omim: 610899 Human
- SwissProt: Q96CF2 Human
- SwissProt: Q9H444 Human
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别名
- C20orf178 antibody
- Charged multivesicular body protein 4b antibody
- Charged multivesicular body protein 4c antibody
see all
图片
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Western blot - Anti-CHMP4B / CHMP4C antibody (ab272638)All lanes : Anti-CHMP4B / CHMP4C antibody (ab272638) at 0.4 µg/ml
Lane 1 : Control (vector only transfected HEK-293T lysate)
Lane 2 : CHMP4B over-expression lysate (mammalian HEK-293T cells) -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CHMP4B / CHMP4C antibody (ab272638)Paraffin-embedded formalin-fixed Human kidney tissue stained for CHMP4B / CHMP4C using ab272638 at 1/500 in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CHMP4B / CHMP4C antibody (ab272638)Paraffin-embedded formalin-fixed Human small intestine tissue stained for CHMP4B / CHMP4C using ab272638 at 1/500 in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CHMP4B / CHMP4C antibody (ab272638)Paraffin-embedded formalin-fixed Human liver tissue stained for CHMP4B / CHMP4C using ab272638 at 1/500 in immunohistochemical analysis.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab272638 被引用在 2 文献中.
- Zhang H et al. CHMP4C as a novel marker regulates prostate cancer progression through cycle pathways and contributes to immunotherapy. Front Oncol 13:1170397 (2023). PubMed: 37388224
- Chen Y et al. Identification of a Pyroptosis-Related Gene Signature and Effect of Silencing the CHMP4C and CASP4 in Pancreatic Adenocarcinoma. Int J Gen Med 15:3199-3213 (2022). PubMed: 35342302