AB167412

重组Anti-CHMP1a抗体[EPR11938(B)]

Anti-CHMP1a antibody [EPR11938(B)]

RabMAb
Recombinant
了解详情

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(2 Publications)

Rabbit Recombinant Monoclonal CHMP1a antibody. Suitable for ICC/IF, WB and reacts with Human, Rat, Mouse samples. Cited in 2 publications.

查看别名

CHMP1, KIAA0047, PCOLN3, PRSM1, CHMP1A, Charged multivesicular body protein 1a, Chromatin-modifying protein 1a, Vacuolar protein sorting-associated protein 46-1, CHMP1a, Vps46-1, hVps46-1

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-CHMP1a antibody [EPR11938(B)] (AB167412)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-CHMP1a antibody [EPR11938(B)] (AB167412)

Immunofluorescent analysis of HeLa cells labeling CHMP1a with ab167412 at 1/250 dilution. DAPI nuclear staining is shown in blue.

Unknown

Western blot - Anti-CHMP1a antibody [EPR11938(B)] (AB167412)

All lanes:

Western blot - Anti-CHMP1a antibody [EPR11938(B)] (ab167412) at 1/1000 dilution

Lane 1:

293T cell lysate at 10 µg

Lane 2:

HeLa cell lysate at 10 µg

Lane 3:

LNCaP cell lysate at 10 µg

Lane 4:

C6 cell lysate at 10 µg

Lane 5:

RAW264.7 cell lysate at 10 µg

Lane 6:

PC12 cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution

Predicted band size: 22 kDa

false

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR11938(B)

亚型

IgG

不含载体蛋白

反应种属

Mouse, Rat, Human

应用

ICC/IF, WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/250 - 1/500", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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产品详情

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

The protein charged multivesicular body protein 1a (CHMP1a) is an essential component of the endosomal sorting complex required for transport (ESCRT)-III also known as chromatin-modifying protein 1A. CHMP1a has a molecular mass of approximately 22 kDa. It shows expression in various tissues with significant presence observed in the brain liver and kidneys. CHMP1a plays a role in the cellular process of membrane remodeling and vesicle trafficking.

Biological function summary

CHMP1a assists in cellular processes like cytokinesis and maintenance of chromatin structure. It is an important part of the ESCRT-III complex which is important for the final stages of cell division and intracellular sorting processes. CHMP1a contributes to the degradation of membrane proteins in lysosomes and the maintenance of proper cell function by controlling the fate of cell surface receptors.

Pathways

The ESCRT-III complex including CHMP1a is involved in the endosomal-lysosomal degradation pathway. This pathway plays a critical role in the down-regulation of cell signaling. CHMP1a associates with proteins such as VPS4 which is involved in the disassembly and recycling of the ESCRT-III complex for repeated use in successive cell cycles. CHMP1a also contributes to the proper functioning of the mitotic spindle assembly ensuring successful mitosis.

Defects or mutations in CHMP1a expression can associate with neurodegenerative diseases like frontotemporal dementia. Research shows implications in cancer with altered CHMP1a expression affecting tumor progression due to disrupted cell division and regulation. The proteins within its pathways such as TSG101 and ALIX also exhibit connections to oncogenic processes further highlighting the role of CHMP1a in pathological conditions.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Probable peripherally associated component of the endosomal sorting required for transport complex III (ESCRT-III) which is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. The MVB pathway appears to require the sequential function of ESCRT-O, -I,-II and -III complexes. ESCRT-III proteins mostly dissociate from the invaginating membrane before the ILV is released. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and the budding of enveloped viruses (HIV-1 and other lentiviruses). ESCRT-III proteins are believed to mediate the necessary vesicle extrusion and/or membrane fission activities, possibly in conjunction with the AAA ATPase VPS4. Involved in cytokinesis. Involved in recruiting VPS4A and/or VPS4B to the midbody of dividing cells. May also be involved in chromosome condensation. Targets the Polycomb group (PcG) protein BMI1/PCGF4 to regions of condensed chromatin. May play a role in stable cell cycle progression and in PcG gene silencing.

See full target information CHMP1A

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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 25: PubMed38928353

2024

Subcellular Localization of Thioredoxin/Thioredoxin Reductase System-A Missing Link in Endoplasmic Reticulum Redox Balance.

Applications

Unspecified application

Species

Unspecified reactive species

Krisztina Veszelyi,Ibolya Czegle,Viola Varga,Csilla Emese Németh,Balázs Besztercei,Éva Margittai

Frontiers in neurology 12:750908 PubMed34975719

2021

Preconditioning With Intermittent Hypobaric Hypoxia Attenuates Stroke Damage and Modulates Endocytosis in Residual Neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Yaqi Wan,Lu Huang,Yanmin Liu,Weizhong Ji,Changxing Li,Ri-Li Ge

View all publications

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Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。

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For licensing inquiries, please contact partnerships@abcam.com

重组Anti-CHMP1a抗体[EPR11938(B)]

Anti-CHMP1a antibody [EPR11938(B)]

Immunocytochemistry/ Immunofluorescence - Anti-CHMP1a antibody [EPR11938(B)] (AB167412)

Western blot - Anti-CHMP1a antibody [EPR11938(B)] (AB167412)

关键信息

宿主种属

克隆

克隆号

亚型

不含载体蛋白

反应种属

应用

免疫原