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Anti-Chk2 (phospho T383)抗体(ab59408)

Reviews (3)Q&A (2)References (12)
Western blot - Anti-Chk2 (phospho T383) antibody (ab59408)
  • Immunocytochemistry - Anti-Chk2 (phospho T383) antibody (ab59408)
  • Immunocytochemistry - Anti-Chk2 (phospho T383) antibody (ab59408)

Key features and details

  • Rabbit polyclonal to Chk2 (phospho T383)
  • Suitable for: ELISA, ICC/IF, ICC, WB
  • Reacts with: Mouse, Rat, Human, African green monkey
  • Isotype: IgG

概述

  • 产品名称

    Anti-Chk2 (phospho T383)抗体
    参阅全部 Chk2 一抗

  • 描述

    兔多克隆抗体to Chk2 (phospho T383)

  • 宿主

    Rabbit

  • 经测试应用

    适用于: ELISA, ICC/IF, ICC, WBmore details

  • 种属反应性

    与反应: Mouse, Rat, Human, African green monkey

  • 免疫原

    Synthetic peptide corresponding to Human Chk2 (phospho T383). (M-R-TP-L-C)
    Database link: O96017

  • 阳性对照

    • WB: COS7 cell extracts, UV treated (30 mins). ICC: Human colon carcinoma cells.

  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab59408于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
ELISA
Use at an assay dependent concentration.
ICC/IF (1)
Use at an assay dependent concentration.
ICC
Use at an assay dependent concentration.
WB (2)
1/500 - 1/1000. Detects a band of approximately 61 kDa (predicted molecular weight: 61 kDa).
说明
ELISA
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.
ICC
Use at an assay dependent concentration.
WB
1/500 - 1/1000. Detects a band of approximately 61 kDa (predicted molecular weight: 61 kDa).

靶标

  • 功能

    Regulates cell cycle checkpoints and apoptosis in response to DNA damage, particularly to DNA double-strand breaks. Inhibits CDC25C phosphatase by phosphorylation on 'Ser-216', preventing the entry into mitosis. May also play a role in meiosis. Regulates the TP53 tumor suppressor through phosphorylation at 'Thr-18' and 'Ser-20'.

  • 组织特异性

    High expression is found in testis, spleen, colon and peripheral blood leukocytes. Low expression is found in other tissues.

  • 疾病相关

    Defects in CHEK2 are associated with Li-Fraumeni syndrome 2 (LFS2) [MIM:609265]; a highly penetrant familial cancer phenotype usually associated with inherited mutations in p53/TP53.
    Defects in CHEK2 may be a cause of susceptibility to prostate cancer (PC) [MIM:176807]. It is a malignancy originating in tissues of the prostate. Most prostate cancers are adenocarcinomas that develop in the acini of the prostatic ducts. Other rare histopathologic types of prostate cancer that occur in approximately 5% of patients include small cell carcinoma, mucinous carcinoma, prostatic ductal carcinoma, transitional cell carcinoma, squamous cell carcinoma, basal cell carcinoma, adenoid cystic carcinoma (basaloid), signet-ring cell carcinoma and neuroendocrine carcinoma.
    Defects in CHEK2 are found in some patients with osteogenic sarcoma (OSRC) [MIM:259500].

  • 序列相似性

    Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CHK2 subfamily.
    Contains 1 FHA domain.
    Contains 1 protein kinase domain.

  • 翻译后修饰

    Phosphorylated by PLK4.

  • 细胞定位

    Nucleus; Nucleus. Isoform 10 is present throughout the cell and Nucleus > PML body. Nucleus > nucleoplasm. Recruited into PML bodies together with TP53.
  • Information by UniProt

  • 数据库链接

    see all

  • 别名

    • CDS 1 antibody
    • Cds1 antibody
    • Cds1 homolog antibody
    • Checkpoint kinase 2 antibody
    • Checkpoint like protein CHK2 antibody
    • CHEK 2 antibody
    • Chek2 antibody
    • Chk 2 antibody
    • CHK2 checkpoint homolog (S. pombe) antibody
    • CHK2 checkpoint homolog antibody
    • CHK2_HUMAN antibody
    • hCds1 antibody
    • HuCds 1 antibody
    • LFS 2 antibody
    • LFS2 antibody
    • PP1425 antibody
    • RAD 53 antibody
    • RAD53 antibody
    • Rad53 homolog antibody
    • Serine/threonine protein kinase Chk2 antibody
    • Serine/threonine-protein kinase Chk2 antibody
    see all

图片

  • Western blot - Anti-Chk2 (phospho T383) antibody (ab59408)
    Western blot - Anti-Chk2 (phospho T383) antibody (ab59408)
    All lanes : Anti-Chk2 (phospho T383) antibody (ab59408) at 1/500 dilution

    Lane 1 : COS7 cell extracts, UV treated (30 mins)
    Lane 2 : COS7 cell extracts, UV treated (30 mins) with immunising phosphopeptide

    Predicted band size: 61 kDa
    Observed band size: 61 kDa

  • Immunocytochemistry - Anti-Chk2 (phospho T383) antibody (ab59408)
    Immunocytochemistry - Anti-Chk2 (phospho T383) antibody (ab59408)

    HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Chk2 (phospho T383) (green) using ab59408 at 1/100 dilution in ICC/IF, in the presence (right panel) or absence (left panel) of immunizing phosphopetide.

  • Immunocytochemistry - Anti-Chk2 (phospho T383) antibody (ab59408)
    Immunocytochemistry - Anti-Chk2 (phospho T383) antibody (ab59408)This image is courtesy of an Abreview submitted by Eleni Petsalaki

    ab59408 staining Chk2 (phospho T383) in human colon carcinoma cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.5% Triton X-100 and blocked with 1% BSA for 30 minutes at 37°C. Samples were incubated with primary antibody (1/100 in PBS + 1% BSA) for 1 hour at 37°C. A FITC-conjugated goat anti-rabbit IgG polyclonal (1/100) was used as the secondary antibody.

    See Abreview

文献 (12)

发表研究结果有使用 ab59408？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab59408 被引用在 12 文献中.

  • Golovine K  et al. ABL1 kinase as a tumor suppressor in AML1-ETO and NUP98-PMX1 leukemias. Blood Cancer J 13:42 (2023). PubMed: 36959186
  • Viswanathan P  et al. Transplanted hepatocytes rescue mice in acetaminophen-induced acute liver failure through paracrine signals for hepatic ATM and STAT3 pathways. FASEB J 35:e21471 (2021). PubMed: 33683737
  • Dias MH  et al. Fibroblast Growth Factor 2 lethally sensitizes cancer cells to stress-targeted therapeutic inhibitors. Mol Oncol 13:290-306 (2019). PubMed: 30422399
  • Liu X  et al. Celastrol mediates autophagy and apoptosis via the ROS/JNK and Akt/mTOR signaling pathways in glioma cells. J Exp Clin Cancer Res 38:184 (2019). PubMed: 31053160
  • Kim SH & Park JW IDH2 deficiency impairs cutaneous wound healing via ROS-dependent apoptosis. Biochim Biophys Acta Mol Basis Dis 1865:165523 (2019). PubMed: 31376482
View all Publications for this product

客户评价及客户问答

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1-5 of 5 Abreviews or Q&A

Immunocytochemistry/ Immunofluorescence abreview for Anti-Chk2 (phospho T383) antibody

 Excellent
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 37°C
Sample
Human Cell (Colon carcinoma cells)
Specification
Colon carcinoma cells
Permeabilization
Yes - 0.5% Triton
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Eleni Petsalaki

Verified customer

提交于 Mar 03 2014

Western blot abreview for Anti-Chk2 (phospho T383) antibody

 Excellent
Abreviews
abreview image
Application
Western blot
Loading amount
50 µg
Gel Running Conditions
Non-reduced Denaturing (8%)
Sample
Human Cell lysate - whole cell (Human Colon carcinoma BE cells)
Specification
Human Colon carcinoma BE cells
Treatment
Etoposide 10 μM for 8 h
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Eleni Petsalaki

Verified customer

提交于 Jan 28 2013

Western blot abreview for Anti-Chk2 (phospho T383) antibody

 Good
Abreviews
abreview image
Application
Western blot
Sample
Xenopus laevis Cell lysate - other (One egg or embryo equivalent loaded per lane)
Loading amount
1 cells
Specification
One egg or embryo equivalent loaded per lane
Treatment
100 µM Bleomycin for 18 hours
Gel Running Conditions
Reduced Denaturing (10% polyacrylamide)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. XXXXXX XXXXXX

Verified customer

提交于 Jan 16 2012

Question

anbei schicke ich Ihnen den ausgefüllten Fragebogen zu dem von uns beanstandeten Antikörper.

Für evtl. Rückfragen stehe ich Ihnen gerne unter xx zur Verfügung.

Sehe Ihrer weiteren Nachricht gerne entgegen.

Vielen Dank für Ihre Mühe!

mfg

Read More
Answer

Vielen Dank für das Senden des ausgefüllten Fragebogens.

Ich habe mir das Protokoll auch mit dem Labor angeschaut.

Das Labor hat keineBanden bei100 oder 150 kD erhalten. Es könnte jedoch sein, dass je nach Probenzubereitung, es noch Aggregate hat in den Proben. Ich habe Ihnen daher im Anhang eine Empfehlung zur Probenzubereitung getan, welche Aggregate und Dimere ausschliessen soll. Vielleicht könnte dies helfen, das Problem zu lösen.

Können Sie mir bitte auch (nochmals)bestätigen, dass der sekundäre Antikörper mit anderen primären Antikörpern gut funktioniert?

Falls dies das Problem nicht lösen wird, werden wir Ihnen den Antikörper gerne ersetzen.

Read More

Question

Phone call: Bands not at the expected 60kD, but at 150kD and about 100kD. Use of irradiated human lymphoblastoid cells, where phsopho T383 should be. Blocked with skim milk and BSA, used TBS, two different secondary antibodies etc.

Read More
Answer

Vielen Dank für Ihren Anruf.

Es tut mir leid zu hören, dass Sie Probleme mit diesem Antikörper haben.

Ich habeden besprochnenFragebogen als Word-Dokument an diese E-Mail angehängt. Durch das Ausfüllen des Fragebogens erhalten wir alle nötigen Informationen über Ihre Proben und Ihr Protokoll. Sobald Sie dieses Formular an uns zurückgeschickt haben, werden wir uns Ihr Protokoll ansehen und uns wieder bei Ihnen melden.Ich wäre Ihnen dankbar, wenn Sie auch einen Scan des Resultates mitschicken könnten.Ich kann dann das Labor anfragen, ob auch andereZellen getestet wurdenundauch Banden bei 100 oder 150kD gesehen wurden.
Falls der Antikörper nicht so funktioniert, wie auf dem Datenblatt beschrieben und er innerhalb der letzten 180 Tage gekauft wurde, werden wir Ihnen gerne einen Ersatzantikörper Ihrer Wahl oder eine Gutschrift/Rückerstattungschicken.

Ich freue mich, bald wieder von Ihnen zu hören.

Read More

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