AB59408

Anti-Chk2 (phospho T383)抗体

Name: Chk2(磷酸化T383)抗体 (ab59408)| Abcam中文官网
Brand: Abcam Limited
SKU: AB59408
Price: 4629 CNY
Availability: InStock
Rating: 5 (3 reviews)

Anti-Chk2 (phospho T383) antibody

(3 Reviews)

(18 Publications)

Rabbit Polyclonal Chk2 phospho T383 antibody. Suitable for ICC, ELISA, WB, ICC/IF and reacts with Human, African green monkey, Mouse, Rat samples. Cited in 18 publications. Immunogen corresponding to Synthetic Peptide within Human CHEK2 phospho T383.

查看别名

CDS1, CHK2, RAD53, CHEK2, Serine/threonine-protein kinase Chk2, CHK2 checkpoint homolog, Cds1 homolog, Checkpoint kinase 2, Hucds1, hCds1

3 Images

AbReview41444****

Immunocytochemistry - Anti-Chk2 (phospho T383) antibody (AB59408)

ab59408 staining Chk2 (phospho T383) in human colon carcinoma cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.5% Triton X-100 and blocked with 1% BSA for 30 minutes at 37°C. Samples were incubated with primary antibody (1/100 in PBS + 1% BSA) for 1 hour at 37°C. A FITC-conjugated goat anti-rabbit IgG polyclonal (1/100) was used as the secondary antibody.

This image is courtesy of an Abreview submitted by Eleni Petsalaki

Supplier Data

Immunocytochemistry - Anti-Chk2 (phospho T383) antibody (AB59408)

HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Chk2 (phospho T383) (green) using ab59408 at 1/100 dilution in ICC/IF, in the presence (right panel) or absence (left panel) of immunizing phosphopetide.

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Western blot - Anti-Chk2 (phospho T383) antibody (AB59408)

All lanes:

Western blot - Anti-Chk2 (phospho T383) antibody (ab59408) at 1/500 dilution

Lane 1:

COS7 cell extracts, UV treated (30 mins)

Lane 2:

COS7 cell extracts, UV treated (30 mins) with immunising phosphopeptide

Predicted band size: 61 kDa

Observed band size: 61 kDa

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关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

反应种属

Human, African green monkey, Mouse, Rat

应用

ICC, WB, ELISA, ICC/IF

applications

免疫原

Synthetic Peptide within Human CHEK2 phospho T383. The exact immunogen used to generate this antibody is proprietary information.

O96017

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "African green monkey": { "ICC-species-checked": "guaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Immunogen

存储溶液

pH: 7 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

运输条件

Blue Ice

储存信息

Stable for 12 months at -20°C

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Serine/threonine-protein kinase which is required for checkpoint-mediated cell cycle arrest, activation of DNA repair and apoptosis in response to the presence of DNA double-strand breaks. May also negatively regulate cell cycle progression during unperturbed cell cycles. Following activation, phosphorylates numerous effectors preferentially at the consensus sequence [L-X-R-X-X-S/T] (PubMed : 37943659). Regulates cell cycle checkpoint arrest through phosphorylation of CDC25A, CDC25B and CDC25C, inhibiting their activity. Inhibition of CDC25 phosphatase activity leads to increased inhibitory tyrosine phosphorylation of CDK-cyclin complexes and blocks cell cycle progression. May also phosphorylate NEK6 which is involved in G2/M cell cycle arrest. Regulates DNA repair through phosphorylation of BRCA2, enhancing the association of RAD51 with chromatin which promotes DNA repair by homologous recombination. Also stimulates the transcription of genes involved in DNA repair (including BRCA2) through the phosphorylation and activation of the transcription factor FOXM1. Regulates apoptosis through the phosphorylation of p53/TP53, MDM4 and PML. Phosphorylation of p53/TP53 at 'Ser-20' by CHEK2 may alleviate inhibition by MDM2, leading to accumulation of active p53/TP53. Phosphorylation of MDM4 may also reduce degradation of p53/TP53. Also controls the transcription of pro-apoptotic genes through phosphorylation of the transcription factor E2F1. Tumor suppressor, it may also have a DNA damage-independent function in mitotic spindle assembly by phosphorylating BRCA1. Its absence may be a cause of the chromosomal instability observed in some cancer cells. Promotes the CCAR2-SIRT1 association and is required for CCAR2-mediated SIRT1 inhibition (PubMed : 25361978). Under oxidative stress, promotes ATG7 ubiquitination by phosphorylating the E3 ubiquitin ligase TRIM32 at 'Ser-55' leading to positive regulation of the autophagosme assembly (PubMed : 37943659).. (Microbial infection) Phosphorylates herpes simplex virus 1/HHV-1 protein ICP0 and thus activates its SUMO-targeted ubiquitin ligase activity.

See full target information CHEK2 phospho T383

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文献 (18)

Recent publications for all applications. Explore the full list and refine your search

eLife 13: PubMed40085681

2025

A microglia clonal inflammatory disorder in Alzheimer's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Rocio Vicario,Stamatina Fragkogianni,Leslie Weber,Tomi Lazarov,Yang Hu,Samantha Y Hayashi,Barbara Craddock,Nicholas D Socci,Araitz Alberdi,Ann Baako,Oyku Ay,Masato Ogishi,Estibaliz Lopez-Rodrigo,Rajya Kappagantula,Agnes Viale,Christine A Iacobuzio-Donahue,Ting Zhou,Richard M Ransohoff,Richard Chesworth,Omar Abdel-Wahab,Bertrand Boisson,Olivier Elemento,Jean-Laurent Casanova,W Todd Miller,Frédéric Geissmann

Cell proliferation 58:e13759 PubMed39477811

2024

Crotonylation of MCM6 enhances chemotherapeutics sensitivity of breast cancer via inducing DNA replication stress.

Applications

Unspecified application

Species

Unspecified reactive species

Haoyun Song,Zhao Guo,Kun Xie,Xiangwen Liu,Xuguang Yang,Rong Shen,Degui Wang

The journal of cardiovascular aging 4: PubMed38455514

2024

Hypoxia-induced stabilization of HIF2A promotes cardiomyocyte proliferation by attenuating DNA damage.

Applications

Unspecified application

Species

Unspecified reactive species

Shah R Ali,Ngoc Uyen Nhi Nguyen,Ivan Menendez-Montes,Ching-Cheng Hsu,Waleed Elhelaly,Nicholas T Lam,Shujuan Li,Abdallah Elnwasany,Yuji Nakada,Suwannee Thet,Roger S Y Foo,Hesham A Sadek

Blood cancer journal 13:42 PubMed36959186

2023

ABL1 kinase as a tumor suppressor in AML1-ETO and NUP98-PMX1 leukemias.

Applications

Unspecified application

Species

Unspecified reactive species

Konstantin Golovine,Gleb Abalakov,Zhaorui Lian,Srinivas Chatla,Adam Karami,Kumaraswamy Naidu Chitrala,Jozef Madzo,Margaret Nieborowska-Skorska,Jian Huang,Tomasz Skorski

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 35:e21471 PubMed33683737

2021

Transplanted hepatocytes rescue mice in acetaminophen-induced acute liver failure through paracrine signals for hepatic ATM and STAT3 pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Preeti Viswanathan,Yogeshwar Sharma,Fadi-Luc Jaber,Tatyana Tchaikovskaya,Sanjeev Gupta

The Journal of cell biology 220: PubMed33355621

2020

An ATM-Chk2-INCENP pathway activates the abscission checkpoint.

Applications

Unspecified application

Species

Unspecified reactive species

Eleni Petsalaki,George Zachos

Biochimica et biophysica acta. Molecular basis of 1865:165523 PubMed31376482

2019

IDH2 deficiency impairs cutaneous wound healing via ROS-dependent apoptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Sung Hwan Kim,Jeen-Woo Park

Journal of experimental & clinical cancer research 38:184 PubMed31053160

2019

Celastrol mediates autophagy and apoptosis via the ROS/JNK and Akt/mTOR signaling pathways in glioma cells.

Applications

Unspecified application

Species

Unspecified reactive species

Xihong Liu,Peiyuan Zhao,Xiujuan Wang,Lei Wang,Yingjun Zhu,Yadi Song,Wei Gao

The Journal of investigative dermatology 139:2016-2028.e7 PubMed30878676

2019

Loss of Epidermal HIF-1α Blocks UVB-Induced Tumorigenesis by Affecting DNA Repair Capacity and Oxidative Stress.

Applications

Unspecified application

Species

Unspecified reactive species

Walid Mahfouf,Mohsen Hosseini,Elodie Muzotte,Martin Serrano-Sanchez,Lea Dousset,François Moisan,Walid Rachidi,Alain Taieb,Jana Rudolf,Hamid Reza Rezvani

Molecular medicine reports 19:1824-1832 PubMed30592261

2018

The novel long non‑coding RNA PRNCR1‑2 is involved in breast cancer cell proliferation, migration, invasion and cell cycle progression.

Applications

Unspecified application

Species

Unspecified reactive species

Danmei Pang,Qian Hu,Xiaoshan Lan,Yingxin Lin,Haibo Duan,Shuo Cao,Yaodong Lin,Litao Li,Feng Peng,Fengtao Pan

View all publications

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