鸡多克隆抗体to GAD67/GAD1 (ab75712)
Key features and details
- Chicken polyclonal to GAD67/GAD1
- Suitable for: IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgY
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
-
产品名称
鸡多克隆抗体to GAD67/GAD1
参阅全部 GAD67/GAD1 一抗 -
宿主
Chicken -
经测试应用
适用于: IHC-P, ICC/IFmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide corresponding to Human GAD1/GAD67 conjugated to keyhole limpet haemocyanin. Corresponding to a region of GAD67 (EC 4.1.1.15) shared between the mouse (P48318), rat (NP_058703) and human (Q99259) gene products
Database link: Q99259-1 -
常规说明
It is important to note that, in our hands, we saw only a subpopulation of GABAergic neurons in various mouse brain structures, including cerebral cortex and cerebellar cortex, labeled with our anti-GAD-67 antibody. This was seen multiple times, and following steps to increase antibody penetration into the tissue. Although the basis of this mysterious subpopulation is unclear, we suspect that the epitope specifically recognized by this antibody on GAD-67 may be cryptic or absent in some inhibitory neurons.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7
Preservative: 0.02% Sodium azide
Constituent: 0.0268% PBS
PBS isotonic (0.9%, w/v) -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
IgY fraction purified from yolks then affinity purified using peptide columns and filter sterilized. -
克隆
多克隆 -
同种型
IgY -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab75712于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
Use at an assay dependent concentration.
|
|
| ICC/IF |
1/1000 - 1/2000.
|
| 说明 |
|---|
|
IHC-P
Use at an assay dependent concentration. |
|
ICC/IF
1/1000 - 1/2000. |
靶标
-
功能
Catalyzes the production of GABA. -
组织特异性
Isoform 3 is expressed in pancreatic islets, testis, adrenal cortex, and perhaps other endocrine tissues, but not in brain. -
疾病相关
Defects in GAD1 are the cause of cerebral palsy spastic quadriplegic type 1 (CPSQ1) [MIM:603513]. A non-progressive disorder of movement and/or posture resulting from defects in the developing central nervous system. Affected individuals manifest symmetrical, non-progressive spasticity and no adverse perinatal history or obvious underlying alternative diagnosis. Developmental delay, mental retardation and sometimes epilepsy can be part of the clinical picture. -
序列相似性
Belongs to the group II decarboxylase family. - Information by UniProt
-
数据库链接
- Entrez Gene: 2571 Human
- Entrez Gene: 14415 Mouse
- Entrez Gene: 24379 Rat
- Omim: 605363 Human
- SwissProt: Q99259 Human
- SwissProt: P48318 Mouse
- SwissProt: P18088 Rat
- Unigene: 420036 Human
see all -
别名
- 67 kDa glutamic acid decarboxylase antibody
- CPSQ1 antibody
- DCE1 antibody
see all
图片
-
Immunocytochemistry/ Immunofluorescence - Chicken polyclonal to GAD67/GAD1 (ab75712)
Immunocytochemistry/Immunofluorescence analysis of e18 mouse neurosphere cells in culture labelling GAD1/GAD67 (green) with ab75712. Cells were fixed with 4% paraformaldehyde. Red - rabbit anti-MAP2, Blue: DAPI nuclear stain.
数据表及文件
-
SDS download
-
Datasheet download
文献 (6)
ab75712 被引用在 6 文献中.
- Nass SR et al. Neurodegeneration Within the Amygdala Is Differentially Induced by Opioid and HIV-1 Tat Exposure. Front Neurosci 16:804774 (2022). PubMed: 35600626
- Shi Y et al. Mouse and human share conserved transcriptional programs for interneuron development. Science 374:eabj6641 (2021). PubMed: 34882453
- Krentzel AA et al. A Membrane G-Protein-Coupled Estrogen Receptor Is Necessary but Not Sufficient for Sex Differences in Zebra Finch Auditory Coding. Endocrinology 159:1360-1376 (2018). PubMed: 29351614
- Bellon A et al. Transdifferentiation of Human Circulating Monocytes Into Neuronal-Like Cells in 20 Days and Without Reprograming. Front Mol Neurosci 11:323 (2018). PubMed: 30760979
- Araújo SES et al. Inflammatory demyelination alters subcortical visual circuits. J Neuroinflammation 14:162 (2017). IHC ; Mouse . PubMed: 28821276
- Mills F et al. Cadherins mediate cocaine-induced synaptic plasticity and behavioral conditioning. Nat Neurosci 20:540-549 (2017). PubMed: 28192395