Anti-CHD4抗体(ab72418)
Key features and details
- Rabbit polyclonal to CHD4
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-CHD4抗体
参阅全部 CHD4 一抗 -
描述
兔多克隆抗体to CHD4 -
宿主
Rabbit -
经测试应用
适用于: WB, IPmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide corresponding to a region between residues 25 and 75 of human CHD4 (NP_001264.2)
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阳性对照
- WB: HeLa, Jurkat and HEK-293T cell lysates; IP: HeLa cell lysate.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
ab72418 was affinity purified using an epitope specific to CHD4 immobilized on solid support. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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ChIP Related Products
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab72418于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/2000 - 1/10000. Detects a band of approximately 280 kDa (predicted molecular weight: 218 kDa).
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| IP |
Use at 2-10 µg/mg of lysate.
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| 说明 |
|---|
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WB
1/2000 - 1/10000. Detects a band of approximately 280 kDa (predicted molecular weight: 218 kDa). |
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IP
Use at 2-10 µg/mg of lysate. |
靶标
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功能
Component of the histone deacetylase NuRD complex which participates in the remodeling of chromatin by deacetylating histones. -
序列相似性
Belongs to the SNF2/RAD54 helicase family.
Contains 2 chromo domains.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain.
Contains 2 PHD-type zinc fingers. -
翻译后修饰
Phosphorylated upon DNA damage, probably by ATM or ATR. -
细胞定位
Nucleus. Cytoplasm > cytoskeleton > centrosome. Associates with centrosomes in interphase. - Information by UniProt
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数据库链接
- Entrez Gene: 1108 Human
- Omim: 603277 Human
- SwissProt: Q14839 Human
- Unigene: 162233 Human
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别名
- ATP dependent helicase CHD4 antibody
- ATP-dependent helicase CHD4 antibody
- CHD 4 antibody
see all
图片
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Western blot - Anti-CHD4 antibody (ab72418)All lanes : Anti-CHD4 antibody (ab72418) at 0.1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lysates/proteins at 50 µg per lane.
Predicted band size: 218 kDa
Exposure time: 3 minutesLysates prepared using NETN lysis buffer.
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Immunoprecipitation - Anti-CHD4 antibody (ab72418)Detection of CHD4 by Western Blot of Immunprecipitate.
ab72418 at 0.1µg/ml staining CHD4 in HeLa whole cell lysate immunoprecipitated using ab72418 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 10 seconds.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (50)
ab72418 被引用在 50 文献中.
- Strathmann EA et al. Epigenetic regulation of plastin 3 expression by the macrosatellite DXZ4 and the transcriptional regulator CHD4. Am J Hum Genet 110:442-459 (2023). PubMed: 36812914
- Lyu X et al. Regulation of CTCF loop formation during pancreatic cell differentiation. Nat Commun 14:6314 (2023). PubMed: 37813869
- El Abdellaoui-Soussi F et al. Interplay between the Chd4/NuRD Complex and the Transcription Factor Znf219 Controls Cardiac Cell Identity. Int J Mol Sci 23:N/A (2022). PubMed: 36076959
- Davidson RK et al. The Chd4 subunit of the NuRD complex regulates Pdx1-controlled genes involved in β-cell function. J Mol Endocrinol 69:329-341 (2022). PubMed: 35521759
- Zhong Y et al. Hierarchical regulation of the resting and activated T cell epigenome by major transcription factor families. Nat Immunol 23:122-134 (2022). PubMed: 34937932