重组Anti-CHD3抗体[EPNCIR110A] (ab109195)
![Western blot - Anti-CHD3 antibody [EPNCIR110A] (ab109195)](https://www.abcam.cn/ps/products/109/ab109195/Images/ab109195-256171-anti-chd3-antibody-epncir110a-western-blot.jpg "Western blot - Anti-CHD3 antibody [EPNCIR110A] (ab109195)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPNCIR110A] to CHD3
- Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF
- Knockout validated
- Reacts with: Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-CHD3抗体[EPNCIR110A]
参阅全部 CHD3 一抗 -
描述
兔单克隆抗体[EPNCIR110A] to CHD3 -
宿主
Rabbit -
经测试应用
适用于: Flow Cyt (Intra), WB, IHC-P, ICC/IFmore details
不适用于: IP -
种属反应性
与反应: Rat, Human
预测可用于: Mouse
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免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- K562, HeLa, C6, and PC12 cell lysates, Human breast and Human ovary tissues; HeLa cells
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This antibody was developed as part of a collaboration between Epitomics, the National Cancer Institute's Center for Cancer Research and the lab of Gordon Hager. View antibodies from NCI Center for Cancer Research Collaboration.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPNCIR110A -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab109195于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt (Intra) |
Use at an assay dependent concentration.
|
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| WB |
1/1000 - 1/10000. Detects a band of approximately 260 kDa (predicted molecular weight: 227 kDa).
|
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| IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
(Heat to 98°C, allow to cool for 10-20 minutes) |
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| ICC/IF |
1/100 - 1/250.
|
| 说明 |
|---|
|
Flow Cyt (Intra)
Use at an assay dependent concentration. |
|
WB
1/1000 - 1/10000. Detects a band of approximately 260 kDa (predicted molecular weight: 227 kDa). |
|
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. (Heat to 98°C, allow to cool for 10-20 minutes) |
|
ICC/IF
1/100 - 1/250. |
靶标
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功能
Component of the histone deacetylase NuRD complex which participates in the remodeling of chromatin by deacetylating histones. Required for anchoring centrosomal pericentrin in both interphase and mitosis, for spindle organization and centrosome integrity. -
组织特异性
Widely expressed. -
序列相似性
Belongs to the SNF2/RAD54 helicase family.
Contains 2 chromo domains.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain.
Contains 2 PHD-type zinc fingers. -
细胞定位
Nucleus. Cytoplasm > cytoskeleton > centrosome. Associates with centrosomes in interphase and mitosis. - Information by UniProt
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数据库链接
- Entrez Gene: 1107 Human
- Entrez Gene: 216848 Mouse
- Entrez Gene: 303241 Rat
- Omim: 602120 Human
- SwissProt: Q12873 Human
- Unigene: 25601 Human
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别名
- 226 antibody
- 2600010P09Rik antibody
- AF020312 antibody
see all
图片
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Western blot - Anti-CHD3 antibody [EPNCIR110A] (ab109195)
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: CHD3 knockout HAP1 cell lysate (20 µg)
Lane 3: Raji cell lysate (20 µg)
Lane 4: Y79 cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab109195 observed at 245 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab109195 was shown to specifically react with CHD3 when CHD3 knockout samples were used. Wild-type and CHD3 knockout samples were subjected to SDS-PAGE. ab109195 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/2000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW)preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CHD3 antibody [EPNCIR110A] (ab109195)](https://www.abcam.cn/ps/products/109/ab109195/Images/ab109195-121813-anti-chd3-antibody-epncir110a-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CHD3 antibody [EPNCIR110A] (ab109195)Immunohistochemical staining of paraffin-embedded Human breast tissue using ab109195 at a dilution of 1/100.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Flow Cytometry (Intracellular) - Anti-CHD3 antibody [EPNCIR110A] (ab109195)](https://www.abcam.cn/ps/products/109/ab109195/Images/ab109195-276947-anti-chd3-antibody-epncir110a-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-CHD3 antibody [EPNCIR110A] (ab109195)Intracellular Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling CHD3 with purified ab109195 at 1/230 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluorr® 488) (ab150077) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) (ab172730) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.
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![Western blot - Anti-CHD3 antibody [EPNCIR110A] (ab109195)](https://www.abcam.cn/ps/products/109/ab109195/Images/ab109195-121814-anti-chd3-antibody-epncir110a-western-blot.jpg)
Western blot - Anti-CHD3 antibody [EPNCIR110A] (ab109195)All lanes : Anti-CHD3 antibody [EPNCIR110A] (ab109195) at 1/1000 dilution
Lane 1 : K562 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : C6 cell lysate
Lane 4 : PC12 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 227 kDa
Observed band size: 260 kDa why is the actual band size different from the predicted? -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CHD3 antibody [EPNCIR110A] (ab109195)](https://www.abcam.cn/ps/products/109/ab109195/Images/ab109195-121812-anti-chd3-antibody-epncir110a-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CHD3 antibody [EPNCIR110A] (ab109195)Immunohistochemical staining of paraffin-embedded Human ovary tissue using ab109195 at a dilution of 1/100.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Immunocytochemistry/ Immunofluorescence - Anti-CHD3 antibody [EPNCIR110A] (ab109195)](https://www.abcam.cn/ps/products/109/ab109195/Images/ab109195-121811-anti-chd3-antibody-epncir110a-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-CHD3 antibody [EPNCIR110A] (ab109195)Immunofluorescent staining of HeLa cells using ab109195 at a dilution of 1/100. -
![Anti-CHD3 antibody [EPNCIR110A] (ab109195)](https://www.abcam.cn/ps/products/109/ab109195/Images/ab109195-7-benefits-of-recombinant-antibodies.png)
Anti-CHD3 antibody [EPNCIR110A] (ab109195)
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab109195 被引用在 3 文献中.
- van der Spek J et al. Inherited variants in CHD3 show variable expressivity in Snijders Blok-Campeau syndrome. Genet Med 24:1283-1296 (2022). PubMed: 35346573
- Xie J et al. The chromatin-remodeling enzyme CHD3 plays a role in embryonic viability but is dispensable for early vascular development. PLoS One 15:e0235799 (2020). PubMed: 32658897
- Rodríguez-Castañeda F et al. The SUMO protease SENP1 and the chromatin remodeler CHD3 interact and jointly affect chromatin accessibility and gene expression. J Biol Chem 293:15439-15454 (2018). PubMed: 30082317